ABSTRACT
Selected aspects of chemico-toxicological analysis of the tranquillizer drug fluoxetine are described. Optimal conditions for fluoxetin extraction from internal organs and biological fluids (blood, urine) are specified and methods proposed for its detection and quantitation including TLC, UV SPM, and HPLC. The proposed methods were verified using laboratory animals and materials for expert examination.
Subject(s)
Antidepressive Agents, Second-Generation , Fluoxetine , Adult , Animals , Antidepressive Agents, Second-Generation/analysis , Antidepressive Agents, Second-Generation/pharmacokinetics , Antidepressive Agents, Second-Generation/toxicity , Antidepressive Agents, Second-Generation/urine , Chromatography, High Pressure Liquid , Chromatography, Thin Layer , Fluoxetine/analysis , Fluoxetine/pharmacokinetics , Fluoxetine/toxicity , Fluoxetine/urine , Humans , Indicators and Reagents , Male , Pharmaceutical Preparations/analysis , Rats , Spectrophotometry, Ultraviolet , Tissue DistributionABSTRACT
Data on Liquid Gas Chromatographic method for detection of the acetic acid after etherification in the cadaveric organs are presented. The results of quantitative acetic acid determination in the cadaveric organs by LGC method after etherification are influenced upon by its "background" values. Acetic acid concentration in the cadaveric organs of subjects who died from the cause other than acetic acid poisoning is within the limits 0.1-0.21 g (mean value 0.17 g) in 100 g of the stomach and 0.07-0.13 g (mean value 0.11 g) in 100 g of the liver. Method may be used in expert practice to determine acetic acid poisoning with due regard for its "background" concentrations in the organs.
