ABSTRACT
A procedure for developing a controlled process for biosynthesis of secondary metabolites is described with reference to rifamycin B as an example. The response of the antibiotic producing culture to changed concentrations of the main nutrients in the initial medium was determined. Mathematical processing of the experimental findings with design of the experiment resulted in defining nutrients such as ammonium sulfate and cornsteep liquor useful for further development of the controlled process.
Subject(s)
Industrial Microbiology/methods , Nocardia/metabolism , Rifamycins/biosynthesis , Ammonium Sulfate/administration & dosage , Ammonium Sulfate/pharmacology , Culture Media , Dose-Response Relationship, Drug , Fermentation/drug effects , Glucose/administration & dosage , Glucose/pharmacology , In Vitro Techniques , Mathematics , Nocardia/drug effects , Nocardia/growth & development , Plant Extracts/administration & dosage , Plant Extracts/pharmacology , Sucrose/administration & dosage , Sucrose/pharmacology , USSR , Zea maysABSTRACT
The study provided data on the rate of consumption of the main nutrients by the rifamycin-producing culture in relation to their content in the initial medium. A program for feeding ammonium sulfate and cornsteep liquor and joint control of both the nutrients was developed and tested. The results of the testing confirmed the practicable precision of the feeding. The control of cornsteep liquor and ammonium sulfate concentrations with the program allowed to increase the yield of rifamycin B by 15 and 20%, respectively. The joint control increased the level of the required product by 25%.