Clustering of bacteria with heterogeneous motility.

We study the clustering of a model cyanobacterium Synechocystis into microcolonies. The bacteria are allowed to diffuse onto surfaces of different hardness and interact with the others by aggregation and detachment. We find that soft surfaces give rise to more microcolonies than hard ones. This effect is related to the amount of heterogeneity of bacteria's dynamics as given by the proportion of motile cells. A kinetic model that emphasizes specific interactions between cells, complemented by extensive numerical simulations considering various amounts of motility, describes the experimental results adequately. The high proportion of motile cells enhances dispersion rather than aggregation.

Slowdown of surface diffusion during early stages of bacterial colonization.

We study the surface diffusion of the model cyanobacterium Synechocystis sp. PCC6803 during the incipient stages of cell contact with a glass surface in the dilute regime. We observe a twitching motility with alternating immobile tumble and mobile run periods, resulting in a normal diffusion described by a continuous-time random walk with a coefficient of diffusion D. Surprisingly, D is found to decrease with time down to a plateau. This is observed only when the cyanobacterial cells are able to produce released extracellular polysaccharides, as shown by a comparative study between the wild-type strain and various polysaccharides-depleted mutants. The analysis of the trajectories taken by the bacterial cells shows that the temporal characteristics of their intermittent motion depend on the instantaneous fraction of visited sites during diffusion. This describes quantitatively the time dependence of D, related to the progressive surface coverage by the polysaccharides. The observed slowdown of the surface diffusion may constitute a basic precursor mechanism for microcolony formation and provides clues for controlling biofilm formation.