[Benign prostate hyperplasia as an age-related problem].

The incidence of genitourological diseases is greater that that of circulatory disorders, benign prostate hyperplasia (BPH) is the commonest urological disease in the elderly and senile males. According to the results of the authors' clinicomorphological study, BPH has been detectable in 69% of the males who died at the age of above 60 years. BPH frequently occurs in patients with arterial hypertension, diabetes mellitus, coronary heart disease, and metabolic syndrome, which necessitates a search for the commonness of causes or mechanisms of development of these diseases. The most important complication of BPH is acute urinary retention. Its major causes in therapeutic clinic are recurrent chronic prostatitis, decompensation of circulatory insufficiency, and emergencies generally resulting from alcohol abuse. As this takes place, the worst prognosis is observed in elderly patients with the complicated comorbid status, particularly in the presence of chronic alcohol intoxication. Patients with BPH are at high and surgical treatment-unassociated risks for pulmonary arterial thromboembolism (PATE). In these patients, the causes of PATE are pelvic deep vein thromboses whose incidence in clinical practice is underestimated.

[Foreign experience in registering and analyzing the poor outcomes of treatment].

Negative consequences of treatment are observed at least in 16% patients at multidisciplinary hospitals and may result from the causes that are independent of medical staff, but more frequently from errors and inadequate treatment standards due to human factors. Systematic improvement of professionalism, which should be started from some educational reforms at medical institutes, is needed.

[Lymphodynamic effect on the condition of iscnemia--reperfusion of the dog small intestine].

Ischemia (30 min)--reperfusion (60 min) of the dog small intestine created by occlusion of the cranial mesenteric artery decreased formation and lymph flow, changed the blood and lymph viscosity and coadulation which had been induced by damaging effect of hypoxia.

Adaptor protein Ruk1 forms protein-protein complexes with endonuclease activity in HEK293 cells.

The structural and functional organization of the adaptor protein Ruk(1) is characterized by the presence of three SH3-domains at the N-terminus followed by Pro- and Ser-rich sequences and a C-terminal coiled-coil region. Multiple modules in the Ruk(1) structure involved in protein-protein interactions can provide for formation of ligand clusters with varied properties and subcellular location. To study the nature and biological role of such complexes, the recombinant protein Ruk(1) with a Glu-epitope at the C-terminus (Ruk(1) Glu-tagged) was purified from transfected HEK293 cells by affinity chromatography on protein G-Sepharose with covalently conjugated anti-Glu-tag antibodies. By SDS polyacrylamide gel electrophoresis with subsequent staining with silver, a set of minor bands in addition to the 85-kD Ruk(1) Glu-tagged was detected in the purified preparation of the recombinant protein. Proteins with affinity for nucleic acids were also revealed in the Ruk(1) Glu-tagged preparation by retardation of electrophoretic mobility of 32P-labeled oligodeoxyribonucleotides in gel. The Ruk(1) Glu-tagged preparation was also shown to hydrolyze both deoxyribonucleotides and plasmid DNA. ZnCl2 and heparin inhibited the DNAse activity. These findings suggest the presence of DNases associated with the Ruk(1) protein in HEK293 cells. Such complexes were isolated from lysates of HEK293 cells by chromatography on heparin-Sepharose. By elution with 0.5 and 1.0 M NaCl, two fractions with DNase activity and containing proteins with molecular weights of 83, 80, and 72 kD were obtained. The reaction was inhibited by ZnCl2 and heparin, and previous precipitation of Ruk-related proteins with anti-Ruk antibodies resulted in the exhaustion of nuclease activity. By immunoblotting with anti-Ruk antibodies, 83-kD protein immunologically related to the Ruk(1) protein was identified in the fractions. It was concluded that the adaptor protein Ruk(1) forms complexes with endonucleases in HEK293 cells.

[Josamycin combination with ranitidine in therapy of gastroduodenal ulcer: clinical efficiency]. / Klinicheskaia éffektivnost' sochetannogo primeneniia dzhozamitsina i ranitidina v terapii iazvennoi bolezni zheludka i dvenadtsatiperstnoi kishki.

Efficacy of a novel eradication treatment with ranitidine, trichopol and jozamycin was compared to that of the standard three-component therapy and monotherapy with ranitidine in 76 patients with exacerbation of gastric and duodenal ulcer. Therapeutic monitoring of ranitidine was performed. In relation to healing of ulcer defect and eradication of Helicobacter pylori, jozamycin is not less effective and more safe than standard three-component therapy. Interaction of the scheme components increases plasma concentration of ranitidine especially in patients with concomitant hepatic and renal diseases.