[Clinical value of determination of the blood elastase activity as a marker of postoperative pancreatitis]. / Klinicheskaia tsennost' opredeliniia aktivnosti élastazy v krovi kak markera posleoperatsionnogo pankreatita.

For diagnosis of postoperative pancreatitis 50 patients were examined after performance of organ-preserving operation for gastroduodenal ulcer disease. In 15 patients various postvagotomy symptoms occurred. In 12 of them the elastase activity in blood was increased already in 12 h after operation, lowering gradually under the influence of specific therapy. On 2-3 days in these patients the amylase activity increase in blood and urine was revealed, testifying the postoperative pancreatitis presence. Under the influence of the treatment conducted the postvagotomy syndromes severity had reduced in accordance with the elastase activity normalization in blood.

[Protective properties of anatoxin obtained from a homogeneous preparation of Pseudomonas aeruginosa exotoxin A]. / Protektivnye svoistva anatoksina, poluchennogo iz gomogennogo preparata ékzotoksina A Pseudomonas aeruginosa.

The protective properties of formulated toxoid obtained from the highly purified preparation of P. aeruginosa exotoxin A have been studied in the test of the active immunization of mice. The study has revealed that the preparation when introduced in 1 or 2 injections in a dose of 15 micrograms, shows faint protective potency with respect to P. aeruginosa strains differing in virulence. Immunization with this toxoid in 3 and 4 injections has been found to ensure 60-100% and 50-60% protection of mice infected with P. aeruginosa toxigenic and proteolytic strains respectively. Immunization with toxoid has been found to induce the appearance of short-term antibacterial immunity which loses its capacity to protect the immunized animals, challenged with both toxigenic and proteolytic P. aeruginosa strains, as early as on day 28. The immunization of mice with toxoid in 4 injections has been shown to induce the development of antitoxic immunity capable of neutralizing up to 150 LD50 of purified exotoxin A.

[Isolation of Pseudomonas aeruginosa exotoxin A by affinity chromatography using specific antibodies]. / Vydelenie ékzotoksin A Pseudomonas aeruginosa metodom affinnoi khromatografii s ispol'zovaniem spetsificheskikh antitel.

The method for the isolation of specific antibodies to P. aeruginosa exotoxin A from rabbit antitoxic serum by means of affinity chromatography has been developed. The conditions permitting the isolation of immunoglobulins to exotoxin A, practically without losses, have been experimentally established. The method for the isolation of exotoxin A from P. aeruginosa culture fluid with the use of specific antibodies to exotoxin A, immobilized on Sepharose 4B, has been worked out. This method makes it possible to obtain 21 mg of exotoxin A per cycle in a column having a working capacity of 25 ml, the yield being 87% of exotoxin A from its initial content in acellular culture fluid. The preparation of exotoxin A thus obtained is toxic, homogeneous and has specific enzymatic activity. Its molecular weight is 71 KD.