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1.
Vet Res ; 51(1): 58, 2020 Apr 29.
Article in English | MEDLINE | ID: mdl-32349781

ABSTRACT

Bovine ephemeral fever is a vector-borne disease of ruminants that occurs in tropical and sub-tropical regions of Africa, Asia and Australia. The disease is caused by a rhabdovirus, bovine ephemeral fever virus (BEFV), which occurs as a single serotype globally. Although several other closely related ephemeroviruses have been isolated from cattle and/or arthropods, only kotonkan virus from Nigeria and (tentatively) Mavingoni virus from Mayotte Island in the Indian Ocean have been previously associated with febrile disease. Here, we report the isolation of a novel virus (Hayes Yard virus; HYV) from blood collected in February 2000 from a bull (Bos indicus) in the Northern Territory of Australia. The animal was suffering from a severe ephemeral fever-like illness with neurological involvement, including recumbency and paralysis, and was euthanised. Histological examination of spinal cord and lung tissue identified extensive haemorrhage in the dura mata with moderate perineuronal oedema and extensive emphysema. HYV displayed cone-shaped morphology, typical of rhabdoviruses, and was found to be most closely related antigenically to Puchong virus (PUCV), isolated in 1965 from mosquitoes in Malaysia. Analysis of complete genome sequences of HYV (15 025 nt) and PUCV (14 932 nt) indicated that each has a complex organisation (3' N-P-M-G-GNS-α1-α2-ß-γ-L 5') and expression strategy, similar to that of BEFV. Based on an alignment of complete L protein sequences, HYV and PUCV cluster with other rhabdoviruses in the genus Ephemerovirus and appear to represent two new species. Neutralising antibody to HYV was also detected in a retrospective survey of cattle sera collected in the Northern Territory.


Subject(s)
Cattle Diseases/virology , Ephemerovirus/isolation & purification , Rhabdoviridae Infections/veterinary , Animals , Cattle , Ephemeral Fever/virology , Male , Northern Territory , Rhabdoviridae Infections/virology
2.
PLoS Negl Trop Dis ; 8(4): e2823, 2014 Apr.
Article in English | MEDLINE | ID: mdl-24762775

ABSTRACT

Despite their importance as vectors of human and livestock diseases, relatively little is known about innate antiviral immune pathways in mosquitoes and other insects. Previous work has shown that Culex Vago (CxVago), which is induced and secreted from West Nile virus (WNV)-infected mosquito cells, acts as a functional homolog of interferon, by activating Jak-STAT pathway and limiting virus replication in neighbouring cells. Here we describe the Dicer-2-dependent pathway leading to WNV-induced CxVago activation. Using a luciferase reporter assay, we show that a NF-κB-like binding site in CxVago promoter region is conserved in mosquito species and is responsible for induction of CxVago expression following WNV infection. Using dsRNA-based gene knockdown, we show that the NF-κB ortholog, Rel2, plays significant role in the signaling pathway that activates CxVago in mosquito cells in vitro and in vivo. Using similar approaches, we also show that TRAF, but not TRAF-3, is involved in activation of Rel2 after viral infection. Overall the study shows that a conserved signaling pathway, which is similar to mammalian interferon activation pathway, is responsible for the induction and antiviral activity of CxVago.


Subject(s)
Culex/virology , Intercellular Signaling Peptides and Proteins/immunology , Ribonuclease III/metabolism , Signal Transduction , Transcription Factors/metabolism , Tumor Necrosis Factor Receptor-Associated Peptides and Proteins/metabolism , West Nile virus/immunology , Animals , Artificial Gene Fusion , Culex/immunology , Genes, Reporter , Immunity, Innate , Intercellular Signaling Peptides and Proteins/metabolism , Luciferases/analysis , Luciferases/genetics , Ribonuclease III/immunology
3.
Proc Natl Acad Sci U S A ; 109(46): 18915-20, 2012 Nov 13.
Article in English | MEDLINE | ID: mdl-23027947

ABSTRACT

Although West Nile virus (WNV) and other arthropod-borne viruses are a major public health problem, the mechanisms of antiviral immunity in mosquitoes are poorly understood. Dicer-2, responsible for the RNAi-mediated response through the C-terminal RNase-III domain, also contains an N-terminal DExD/H-box helicase domain similar to mammalian RIG-I/MDA5 which, in Drosophila, was found to be required for activation of an antiviral gene, Vago. Here we show that the Culex orthologue of Vago (CxVago) is up-regulated in response to WNV infection in a Dicer-2-dependent manner. Further, our data show that CxVago is a secreted peptide that restricts WNV infection by activation of the Jak-STAT pathway. Thus, Vago appears to function as an IFN-like antiviral cytokine in mosquitoes.


Subject(s)
Culex/immunology , Cytokines/immunology , Immunity, Innate/physiology , Insect Proteins/immunology , Janus Kinases/immunology , STAT Transcription Factors/immunology , West Nile virus/immunology , Animals , Chlorocebus aethiops , Cricetinae , Culex/metabolism , Culex/virology , Cytokines/metabolism , DEAD-box RNA Helicases/immunology , DEAD-box RNA Helicases/metabolism , Drosophila melanogaster , Humans , Insect Proteins/metabolism , Janus Kinases/metabolism , RNA, Viral/immunology , RNA, Viral/metabolism , Ribonuclease III/immunology , Ribonuclease III/metabolism , STAT Transcription Factors/metabolism , Vero Cells , West Nile Fever/immunology , West Nile Fever/metabolism , West Nile Fever/transmission , West Nile virus/metabolism
4.
Virology ; 433(1): 236-44, 2012 Nov 10.
Article in English | MEDLINE | ID: mdl-22925335

ABSTRACT

Kimberley virus (KIMV) is an arthropod-borne rhabdovirus that was isolated in 1973 and on several subsequent occasions from healthy cattle, mosquitoes (Culex annulirostris) and biting midges (Culicoides brevitarsis) in Australia. Malakal virus (MALV) is an antigenically related rhabdovirus isolated in 1963 from mosquitoes (Mansonia uniformis) in Sudan. We report here the complete genome sequences of KIMV (15442 nt) and MALV (15444 nt). The genomes have a similar organisation (3'-l-N-P-M-G-G(NS)-α1-α2-ß-γ-L-t-5') to that of bovine ephemeral fever virus (BEFV). High levels of amino acid identity in each gene, similar gene expression profiles, clustering in phylogenetic analyses of the N, P, G and L proteins, and strong cross-neutralisation indicate that KIMV and MALV are geographic variants of the same ephemerovirus that, like BEFV, occurs in Africa, Asia and Australia.


Subject(s)
Ephemerovirus/genetics , Gene Expression , Genome, Viral , Africa , Amino Acid Sequence , Animals , Australia , Cattle , Cell Line , Cricetinae , Ephemerovirus/classification , Ephemerovirus/isolation & purification , Gene Expression Profiling , Molecular Sequence Data , Multigene Family , Phylogeny , Phylogeography , Sequence Alignment , Sequence Homology, Amino Acid
5.
Virology ; 425(2): 143-53, 2012 Apr 10.
Article in English | MEDLINE | ID: mdl-22305623

ABSTRACT

Kotonkan virus (KOTV) and Obodhiang virus (OBOV) are rhabdoviruses that were isolated from arthropods in Africa and formerly classified as lyssaviruses. KOTV causes clinical bovine ephemeral fever in cattle; the ecology and pathogenicity of OBOV is poorly understood. In this paper, we report the complete genome sequences of KOTV and OBOV, their gene expression profiles, and their serological and phylogenetic relationships to other rhabdoviruses. The 15,870 nt KOTV genome (3'-l-N-P-M-G-G(NS)-α1-α2-ß-γ-δ-L-t-5') is similar to that of bovine ephemeral fever virus but encodes an additional protein (δ) that shares homology with the pleckstrin homology domain of coactivator-associated arginine methyltransferase. The 14,717 nt OBOV genome (3'-l-N-P-M-G-G(NS)-α1-α2-ß-L-t-5') is similar to that of Adelaide River virus from which it is distinguishable serologically. In each virus, all ORFs, except α1 and α2, are transcribed as monocistronic mRNA. Genetic and serological data indicate that KOTV and OBOV should be classified as new species in the genus Ephemerovirus.


Subject(s)
Arthropods/virology , Cattle Diseases/virology , Genome, Viral , Rhabdoviridae Infections/veterinary , Rhabdoviridae/genetics , Africa , Amino Acid Sequence , Animals , Base Sequence , Cattle , Cell Line , Molecular Sequence Data , Phylogeny , Rhabdoviridae/chemistry , Rhabdoviridae/classification , Rhabdoviridae/isolation & purification , Rhabdoviridae Infections/virology , Sequence Alignment , Viral Proteins/chemistry , Viral Proteins/genetics , Viral Proteins/metabolism
6.
PLoS One ; 6(1): e14576, 2011 Jan 25.
Article in English | MEDLINE | ID: mdl-21283623

ABSTRACT

BACKGROUND: The cane toad, Bufo (Chaunus) marinus, is one of the most notorious vertebrate pests introduced into Australia over the last 200 years and, so far, efforts to identify a naturally occurring B. marinus-specific pathogen for use as a biological control agent have been unsuccessful. We explored an alternative approach that entailed genetically modifying a pathogen with broad host specificity so that it no longer caused disease, but carried a gene to disrupt the cane toad life cycle in a species specific manner. METHODOLOGY/PRINCIPAL FINDINGS: The adult beta globin gene was selected as the model gene for proof of concept of autoimmunity as a biocontrol method for cane toads. A previous report showed injection of bullfrog tadpoles with adult beta globin resulted in an alteration in the form of beta globin expressed in metamorphs as well as reduced survival. In B. marinus we established for the first time that the switch from tadpole to adult globin exists. The effect of injecting B. marinus tadpoles with purified recombinant adult globin protein was then assessed using behavioural (swim speed in tadpoles and jump length in metamorphs), developmental (time to metamorphosis, weight and length at various developmental stages, protein profile of adult globin) and genetic (adult globin mRNA levels) measures. However, we were unable to detect any differences between treated and control animals. Further, globin delivery using Bohle iridovirus, an Australian ranavirus isolate belonging to the Iridovirus family, did not reduce the survival of metamorphs or alter the form of beta globin expressed in metamorphs. CONCLUSIONS/SIGNIFICANCE: While we were able to show for the first time that the switch from tadpole to adult globin does occur in B. marinus, we were not able to induce autoimmunity and disrupt metamorphosis. The short development time of B. marinus tadpoles may preclude this approach.


Subject(s)
Autoimmunity , Bufo marinus/virology , Host Specificity/immunology , Life Cycle Stages/immunology , Pest Control, Biological/methods , Viruses/genetics , Animals , Host-Pathogen Interactions/immunology , Larva/immunology , Larva/virology , Species Specificity
7.
Biotechniques ; 37(1): 104-6, 108-11, 2004 Jul.
Article in English | MEDLINE | ID: mdl-15283207

ABSTRACT

Here we describe plasmid vectors and selection protocols developed to allow the construction of recombinant fowlpox viruses (rFPVs) with up to three insertions of foreign DNA in the viral genome. Transient dominant selection allows the construction of recombinant viruses that do not retain the selection markers and can therefore be used for the insertion of additional genes at other sites in the viral genome. A SYBR Green real-time PCR sequence detection assay was applied to the identification of recombinant viruses from individual plaques, eliminating the need for amplification and hybridization from the transient dominant protocol and resulting in significant savings in time at each round of plaque purification. Dominant selection techniques allow more rapid recombinant virus construction; however, as the markers are retained along with the gene of interest, they can only be used to generate the final recombinant. rFPV vaccines constructed using these techniques have reached preclinical nonhuman primate and phase I human clinical trials in prime/boost vaccination studies as human immunodeficiency virus (HIV) therapeutic andprophylactic vaccines.


Subject(s)
Fowlpox virus/genetics , Recombination, Genetic , Base Sequence , DNA Primers , Polymerase Chain Reaction
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