ABSTRACT
The conditions of doxilamine isolation from biological fluids are studied. The method of its extraction with a mixture of organic solvents in pH 9 is proposed. Identification of doxilamine with techniques of thin layer chromatography, UV-, IR-spectroscopy, chromato-mass-spectrometry, densitometry, gas-liquid chromatography is described. UV-spectrometry, gas chromatography and densitometry can be used for quantitation of doxilamine.
Subject(s)
Doxylamine/analysis , Forensic Toxicology/methods , Hypnotics and Sedatives/analysis , Substance Abuse Detection/methods , Animals , Doxylamine/blood , Doxylamine/urine , Humans , Hypnotics and Sedatives/blood , Hypnotics and Sedatives/urine , Mice , Pharmaceutical Preparations/chemistry , Pharmaceutical Preparations/standards , Rats , Solvents/chemistry , TabletsABSTRACT
The authors present a method of hingamine identification in non-biological substances (tablets, powder, syringes) and biological fluids (blood, urine). Isolation was made with chloroform in pH 10. Identification was conducted with thin-layer chromatography, gas chromatography/mass-spectrometry, high-performance liquid chromatography, spectrophotometry in UV region. The quantity was estimated with spectrophotometry in UV region, high-performance liquid chromatography and high-performance thin-layer chromatography. The results of the three methods are comparable.
