1.
Cancer Lett
; 23(2): 135-43, 1984 Jun.
Article
in English
| MEDLINE
| ID: mdl-6378361
ABSTRACT
Five low-dose applications of a commercial coal tar preparation on a small scalp skin region resulted in an induction of aryl hydrocarbon hydroxylase (AHH) activity in freshly isolated human hair follicles. Large but reproducible interindividual differences in AHH-inducibility could be detected. The method offers the opportunity to measure AHH-inducibility, which has been correlated to the risk of developing chemical-induced cancer, in vivo in normal epithelium, a cell-type highly relevant for chemical carcinogenesis. Smoking habits did not have any effect on AHH-activity in freshly isolated hair follicles. Therefore the method potentially permits the identification of persons with high and low genetically determined AHH-inducibility.
Subject(s)
Aryl Hydrocarbon Hydroxylases/biosynthesis , Coal Tar/pharmacology , Hair/enzymology , Scalp/enzymology , Administration, Topical , Adult , Aryl Hydrocarbon Hydroxylases/genetics , Carcinoma, Bronchogenic/diagnosis , Clinical Enzyme Tests , Enzyme Induction/drug effects , Epithelium/enzymology , Humans , Kinetics , Lung Neoplasms/diagnosis , Risk , Smoking , Spectrometry, Fluorescence , Subtraction Technique
2.
Anal Biochem
; 125(2): 370-5, 1982 Sep 15.
Article
in English
| MEDLINE
| ID: mdl-7181095