[Morphologic characteristics of the testes in breeding rams after experimental loading with industrial emissions containing copper]. / Morfologický obraz semenníkov plemenných baranov po experimentálnej zátazi priemyselnou emisiou s obsahom medi.

The aim of this work was to experimentally evaluate the effect of feeding industrial emissions from a copper-producing plant upon Cu accumulation in the testes of six Merino breeding rams. In the experimental animals (4) the daily copper intake from the exhalations was 15 mg/kg l.w. Administration was continued until the haemolytic crisis appeared (on day 42 in one animal and on day 50 of the experiment in the other animals). The tissue of the testes was examined by both optic and electron microscopy. The patho-anatomical picture of the organs was characterized by general icterus which was also evident on the sectional surfaces of the testes. In the right and left testis of the experimental animals Cu levels were found that were twice higher than those of the controls (Tab. IV). The toxic effect of copper became evident by the destruction of all developmental stages of germinative cells mainly by that the integrity of the cell membranes was damaged thus resulting in a gradual release of the germinative cells, into the tubular lumen. In extreme cases the tubules were lined with nuclei only and the residual cytoplasm of Sertoli cells (Sertoli cells--only syndrome). Ruptures of the cytoplasmic membranes were also observed on the Leydig cells. Thickening of the tubular lamina propria was caused by homogenization and enormous invagination of the lamellar layer, which was rather predominant on the periphery of the organ (Fig. 2). In the deeper layers of the testes reduction of the cellular and non-cellular layers of the tubular walls occurred which frequently resulted in the rupture of the basement membrane.(ABSTRACT TRUNCATED AT 250 WORDS)

Histological and submicroscopical findings on the seminiferous parenchyma in rams after copper oxide intoxication from industrial emissions.

In this work the author examined the histological and submicroscopical picture of the seminiferous parenchyma in 5 breeding rams from a herd in which intoxication with copper oxide from industrial emissions of a copper producing plant had been diagnosed. The toxic effect irreversibly damages all developmental stages of the germ cells. It injures the cell membranes of both germ cells and Sertoli cells. Similar changes were observed on the interstitial cells. The degree of injury to the germinative epithelium confirms its significant role in the decreased reproduction rate of the herd under examination.

Regeneration of the seminiferous tubules after curettage of the testicular parenchyme in young rams.

I. The findings of seminiferous tubules after total curettage of the testicular parenchyme confirm the previous studies of the author on the origin of regenerates whose source are the cells of the walls of the seminiferous tubules, that are parts of the Moskoff's islets in the tunica albuginea. 2. In the 7th month after operation, in the first place the interstitial components of the parenchyme are substituted. The submicroscopical finding shows hyperplasia of interstitial cells from undifferentiated stages, through modified fibroblasts up to the adult Leydig cell. 3. The findings in the 10th month after operation confirm the regeneration of the seminiferous tubules in which Sertoli cells and spermatogonias are present. No higher stages of germinal cells were observed. A pecularity of the submicroscopical picture at that time is the reduplication of the lamellar layer of the tubular wall with a great number of foldings. In the interstitium Leyding cells were observed.

[A comparison of submicroscopic changes in the testes of rams after experimental ischemia with findings in orchitis in breeding rams]. / Porovnanie submikroskopických zmien na semenníkoch baranov po experimentálnej ischémii zl'azy s nálezmi po orchitídach plemenných baranov v praxi.

Submicroscopic changes in the testes of rams were studied after experimental ischaemia induced by ligature on a. testicularis. The obtained findings were compared with those after traumatic orchitis in breeding rams. Both groups of animals showed marked changes in the germinal as well as Sertoli cells, which were characterized by generalized vacuolization of cell cytoplasm, accompanied by cell caryolysis. The Sertoli cells showed lipid cumulation and glycogen multiplication. The tubule wall had undulated layers. A pronounced reduplication of the lamellar layer of the tubule wall was recorded in the rams with orchitis. The interstitial tissue shows multiplication of collagenous fibres. The findings warn against traumatic injury of testes. The disordered spermiogenesis is irreversible.

[Changes in the seminiferous parenchyma of rams after experimental ischemia]. / Zmeny na semenotvornom parenchýme baranov po experimentálnej ischémii.

The changes induced by experimental ligature of a. testicularis and its effect on the parenchyma of the testes were studied in juvenile rams. Short-term ligature (four days) causes destructive changes on gonocytes and precursors of Sertoli's cells which show considerable vacuolization of cytoplasm. The later post-operative period is characterized by the cytolysis and caryolysis of all cells. In a period of three to four months there is fibration of the necrotically altered organ. Traumatic damage caused to the sexual gland leads to a disorder in the circulation of blood, resulting in the necrosis of all components of testicular parenchyma, except tunica albuginea.