ABSTRACT
The male adult of Chaetocladius (Chaetocladius) spiridonovi sp. nov. is described and diagnosed based on morphological and phylogenetic data. The new species is characterized by the following features: anal point very long, almost reaching apex of gonocoxite, presence of a dorsal hump at base; inferior volsella sub-rectangular to large lobe shaped, inner and caudal margin with undulation; virga composed of 2 long spines; gonostylus massively globular with rounded posterior margin. Comparison of COI with that of other known Chaetocladius species shows a K2P genetic distances of 8.9220.31%, values well associated with interspecific variation. The barcodes of the new species were identical to that of Chaetocladius sp. 7ES in BOLD systems. Molecular data were also used for the reconstruction of the phylogenetic relationships within the subgenus Chaetocladius.
Subject(s)
Chironomidae , Diptera , Lepidoptera , Animals , Chironomidae/genetics , Male , Phylogeny , RussiaABSTRACT
Chironomids of the Diamesinae subfamily from the Russian Arctic were studied using both morphological characters and molecular data. Adult males of Diamesa urvantsevi sp. nov., D. amplexivirilia Hansen, Arctodiamesa appendiculata (Lundström) from Severnaya Zemlya Archipelago and D. arctica (Boheman), Pseudokiefferiella sp. from Vaigach Island were described, redescribed, annotated and figured. A reference 658 bp barcode sequence from a fragment of the mitochondrial gene cytochrome oxidase I (COI) was used as a tool for species delimitation. For D. arctica (Boheman) and Pseudokiefferiella sp. close DNA barcodes from Norway were performed, which allowed to relate these specimens to the described species. Comparisons with corresponding regions of COI between each described species and close related congeneric species produced K2P genetic distances of 0.11-0.16, values well associated with interspecific variation. Phylogenetic relationships for genera Arctodiamesa Makarchenko and Pseudokiefferiella Zavrel were reconstructed for the first time.
