[The epidemic importance of the virological study of sewage for rotaviruses]. / Epidemicheskaia znachimost' virusologicheskogo issledovaniia stochnykh vod na rotavirusy.

The results of correlation of rotavirus circulation indices among apparently normal and sick children and the rates of rotavirus antigen detection in sewage from an orphanage and an infectious ward of children's hospital are presented. It was established that there should be up to 1% of virus excretors for rotaviruses to be detectable in sewage of peripheral collectors of children's institutions. A significant increase in the rate of detection of rotaviruses in sewage may indicate an activation of the epidemic process of rotavirus infection.

[The results of a study of the contamination of bodies of water by rotaviruses against a background of gastroenteritis morbidity]. / Rezul'taty izucheniia kontaminatsii vodnykh ob''ektov rotavirusami na fone zabolevaemosti gastroénterita.

The results of a sanitary virological survey of the prevalence of rotaviruses in water objects in relation to gastroenteritis incidence are presented. Some regularities of the epidemic process of rotavirus infection were discovered: a high portion of infants involved, occurrence of cases of the disease predominantly in the cold periods of the year, a significant portion (23.8%) of rotavirus gastroenteritis in the total incidence of acute intestinal infections, etc. Besides, comparison of the data on the dynamics of rotavirus infection incidence and on the contamination of water objects with rotaviruses revealed a strong correlation between these data. The possibility of using this information for the evaluation of the epidemiological situation is discussed.

[The possibility for the sanitary removal of enteroviruses from water reservoirs in the case of intensive irrigation agriculture]. / Izuchenie vozmozhnosti sanatsii vodnykh ob''ektov ot kishechnykh virusov v usloviiakh intensivnogo oroshaemogo zemledeliia.

The efficacy of elimination of enteric viruses from water in the process of exploitation of a closed irrigating system is evaluated. Experimental and natural observations showed the half-life of the index virus in the water objects of the irrigating system to be 1.57 days. The potentials of using a temperature-dependent variant of poliomyelitis virus as the internal control in studies of the prevalence of enteric virules among human subjects and in the environment under conditions of intensive irrigated agriculture are considered.

[Computer model for prognosis of incidence of aseptic meningitis]. / Komp'iuternaia model' prognozirovaniia zabolevaemosti seroznym meningitom.

A possibility of construction of a mathematical model for current prognosing of aseptic meningitis incidence both for 2-4 months in advance and for the whole year has been demonstrated. The initial information consisted of the data on prevalence of enteroviruses in water objects as from May. Comparison of the actual and estimated incidence indicates that with the proposed model, up to 90.4% of the incidence of aseptic meningitis may be explained.

[Survival of the poliomyelitis virus in environmental objects]. / Izuchenie vyzhivaemosti virusa poliomielita v nekotorykh ob''ektakh vneshnei sredy.

The guanidine-dependent variant of poliovirus type I was shown to survive in soil and on tomato plants for 60 and 25 days, and in the roots, stems, leaves for 15, 20, and 8 days, respectively. Extrapolation of the significance of these results indicates that the decline of concentration of the indicator virus in the soil and on the vegetables and its increase and decline in the root system, stems, leaves, and in tomatoes is of exponential nature.

[Correlation of serous meningitis morbidity with the prevalence of enteroviruses in water]. / Sopostavlenie zabolevaemosti seroznym meningitom s rasprostranennost'iu énterovirusov v vodnykh ob''ektakh.

Investigation of the relationship between the incidence of aseptic meningitis etiologically due to Coxsackie B1 virus and circulation of enteroviruses in the water indicates not only a correlation between enteroviruses isolation from sewage and from patients, but also a direct relationship between the number of virus excretors and prevalence of enteroviruses in open water bodies in respect of seasonal distribution, type composition, frequency of detection and concentration of viruses in different epidemiological situations.

[The level of herd immunity against poliomyelitis in children during the mass vaccinal prophylaxis of this infection]. / Izuchenie urovnia kollektivnogo immuniteta k poliomielitu u detei v usloviiakh massovoi vaktsinoprofilaktiki étoi infektsii.

The results of examinations of blood sera from normal children for the presence of antibody to polioviruses after two courses of mass vaccinations were carried out according to epidemiological indications although differing in duration of the campaign and the range of the child populations involved, are presented. The advantages of simultaneous mass vaccinations covering children from 2 months to 7 years of age have been demonstrated.

[Comparison of serous meningitis morbidity and the concentration of the Coxsackie B virus in sewage]. / Sopostavlenie zabolevaemosti seroznym meningitom i kontsentratsii virusa Koksaki B v stochnykh vodakh.

The paper shows that the frequency of detection and concentration of Coxsackie B virus in sewage correlate with the number of patients and asymptomatic virus excretors.

[Detection of Coxsackie virus B in sewage and a correlation with serous meningitis morbidity]. / Obnaruzhenie virusa Koksaki B v stochnykh vodakh i sopostavlenie s zabolevaemost'iu seroznym meningitom.

The results of parallel virological examinations of sewage from collectors of individual districts, aeration stations, and materials from patients with serous meningitis for 1979-1982 are presented. An attempt was made to determine the relationship between the frequency of detection of enteroviruses, in particular, Coxsackie B1, in sewage and the number of patients with serous meningitis. Enteroviruses isolated from sewage and from patients with serous meningitis were found to be similar by the type composition and seasonal distribution; there was also a relationship between the frequency of their finding in sewage and the number of cases of serous meningitis.

[Isolation of enteroviruses from patients with clinical manifestations of poliomyelitis]. / Vydelenie énterovirusov ot bol'nykh s klinicheskimi proiavleniiami poliomielita.

Examinations of 114 specimens (feces, nasopharyngeal washings, cerebrospinal fluid) from patients with clinical manifestations of poliomyelitis were carried out. Enteroviruses were isolated in 61.4% of the cases. Among the isolates, poliomyelitis viruses accounted for 49.1%, and Coxsackie B1 and ECHO 6 12.3%. Typing of the poliovirus isolates showed 17.9% to belong to type I, 7.1% to type II, and 75% to type III. Studies on intratypic differentiation revealed their antigenic similarity with the Mahoney, MEF-1, and Saukett strains, respectively. Among 24 paired blood sera tested for virus-neutralizing antibodies to wild poliovirus strains 21 demonstrated a 4-fold or higher rise of antibody titres to the above poliomyelitis viruses.

[Characteristics of poliomyelitis viruses isolated from sewage]. / Izuchenie kharaketritiki virusov poliomielita, vydelennykh iz stochnykh vod.

Studies of the genetic markers of 39 (57.4%) strains of poliomyelitis virus type I isolated from sewage showed 7 of them to have genetic markers ret+40, Gu+, A+, Ag+ (Mahoney, Koñkov), 3 to be ret+40, Gu+, A+, Ag+ (Koñkov, Sabin), and 10 to be ret+40, Gu-, A-, Ag- (LSc 2ab). Among 20 (29.4%) strains of poliovirus type II two were ret+40, Gu+, A+, N+, Ag+ (MEF-1), and 2 were ret-40, Gu-, A-, N-, Ag- (P712 Ch2ab), and among 9 (15.2%) strains of type III only one (Saukett) had the genetic markers ret+40, Gu+, A+, Ag+. In the other 43 poliovirus strains under study the genetic markers did not correlate with each other. The possibility of using counter immunoelectrophoresis alongside with gel precipitation test for the study of antigenic intratype differences of polioviruses was demonstrated.