Condition of fagocytosis of experimental animals with periodontitis due to modified reactivity.

OBJECTIVE: Introduction: Taking into account the patho-immune mechanisms of formation of inflammatory process in periodontium, there is a necessity for in-depth study of the pathogenesis of periodontal diseases from the position of changes in the reactivity of the organism. The aim: Carrying out a comparative estimation of phagocytic activity of leukocytes of animals with inflammation in periodontium with altered reactivity. PATIENTS AND METHODS: Materials and methods: Experiments were performed on 30 white rats: Group I - 10 white rats with hypoergic reaction; Group ІІ - 10 white rats with hyperergic reaction; Group III - 10 white rats with normergic reaction - control group. Slaughter and blood sampling under thiopental anesthesia was performed 7 days after the beginning of the experiment. The percentage of phagocytic leukocytes - phagocytic index, phagocytic number were determined and the index of phagocytic activity was calculated. RESULTS: Results: The phagocytic index (Fi,%) decreased by 2,09 times (p <0,05) at hypoergic, it increased by 1,37 times (p <0,05) at hyperergic; the index of phagocytic activity (IFA) decreased by 1.96 times at hypoergic (p <0.05); growth was 1.94 times (p <0.05) in the hyperergic group of animals. In both experimental groups, the phagocyte number (Fu) increased by 6.25% and 41.7%, with hypoergic and hyperergic, respectively. CONCLUSION: Conclusions: Different directions of changes of the phagocytic activity were observed: increase of these parameters at hyperergic and decrease at hypoergic. An increase in phagocyte number was likely to indicate some autonomy of the process of phagocytosis and independence from the reactivity of the organism.

The state of oxidative homeostasis in children with influenza stomatitis.

OBJECTIVE: Introduction: The clinical course of acute respiratory viral infections was not sufficiently studied, specially the state of oxidative homeostasis in children with influenza stomatitis. This fact became the base for our study. The aim: to characterize the state of oxidative-prooxidant system as one of the factors of non-specific resistance of children` organism with influenza stomatitis. PATIENTS AND METHODS: Materials and methods: A survey was conducted on 384 children with acute respiratory viral infections aged from 6 months to 12 years, among them 318 had lesions of oral cavity. The mild form was diagnosed in 52 children, moderately severe - in 185, severe - in 81 children. The control group consisted of children without lesions of oral cavity (66 people). To analyze lipid peroxidation we used a spectrophotometric determination of diene conjugates. The ceruloplasmin activity and the transferrin saturation in blood plasma by iron were determined by G. Babenko's method. RESULTS: Results: We found the intensification of lipid peroxidation: a significant increase of diene conjugates in serum up to 13.78 %, the level of which depended on the severity of disease. We also found the activity increase of ceruloplasmin in 1,8 times in patients with severe course of disease. The saturation of blood plasma transferrin by iron was significantly reduced - for 15.27 % in patients with severe course of influenza stomatitis. CONCLUSION: Conclusions: Changes in antioxidant system happend due to the activation of lipid peroxidation, and because of the inability to neutralize toxic metabolites in the children` body the intoxication syndrome developed.