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Bioorg Khim ; 21(7): 492-7, 1995 Jul.
Article in Russian | MEDLINE | ID: mdl-7488263

ABSTRACT

The gene of ribosomal protein S8 from the extreme thermophile Thermus thermophilus was expressed in E. coli using the strain BL21(DE3) and vector pET3-1. A method of isolating this protein from the super producing strain was developed, which makes it possible to obtain 8-12 mg of product from 11 of culture. The secondary structure of protein S8 was determined by using CD spectroscopy. The protein was shown to be highly resistant to denaturants.


Subject(s)
Ribosomal Proteins/chemistry , Thermus thermophilus/metabolism , Amino Acid Sequence , Circular Dichroism , Cloning, Molecular , Escherichia coli/genetics , Genetic Vectors , Molecular Sequence Data , Protein Denaturation , Ribosomal Proteins/genetics , Ribosomal Proteins/isolation & purification , Thermodynamics , Thermus thermophilus/genetics
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