1.
Plant Physiol
; 127(2): 386-9, 2001 Oct.
Article
in English
| MEDLINE
| ID: mdl-11598214
2.
Bioorg Khim
; 21(7): 492-7, 1995 Jul.
Article
in Russian
| MEDLINE
| ID: mdl-7488263
ABSTRACT
The gene of ribosomal protein S8 from the extreme thermophile Thermus thermophilus was expressed in E. coli using the strain BL21(DE3) and vector pET3-1. A method of isolating this protein from the super producing strain was developed, which makes it possible to obtain 8-12 mg of product from 11 of culture. The secondary structure of protein S8 was determined by using CD spectroscopy. The protein was shown to be highly resistant to denaturants.