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1.
Mol Microbiol ; 121(5): 850-864, 2024 05.
Article in English | MEDLINE | ID: mdl-38323722

ABSTRACT

The diarrheal disease cholera is caused by the versatile and responsive bacterium Vibrio cholerae, which is capable of adapting to environmental changes. Among others, the alternative sigma factor RpoS activates response pathways, including regulation of motility- and chemotaxis-related genes under nutrient-poor conditions in V. cholerae. Although RpoS has been well characterised, links between RpoS and other regulatory networks remain unclear. In this study, we identified the ArcAB two-component system to control rpoS transcription and RpoS protein stability in V. cholerae. In a manner similar to that seen in Escherichia coli, the ArcB kinase not only activates the response regulator ArcA but also RssB, the anti-sigma factor of RpoS. Our results demonstrated that, in V. cholerae, RssB is phosphorylated by ArcB, which subsequently activates RpoS proteolysis. Furthermore, ArcA acts as a repressor of rpoS transcription. Additionally, we determined that the cysteine residue at position 180 of ArcB is crucial for signal recognition and activity. Thus, our findings provide evidence linking RpoS response to the anoxic redox control system ArcAB in V. cholerae.


Subject(s)
Bacterial Proteins , Gene Expression Regulation, Bacterial , Sigma Factor , Vibrio cholerae , Vibrio cholerae/genetics , Vibrio cholerae/metabolism , Bacterial Proteins/metabolism , Bacterial Proteins/genetics , Sigma Factor/metabolism , Sigma Factor/genetics , Phosphorylation , Transcription Factors/metabolism , Transcription Factors/genetics , Chemotaxis/genetics , Repressor Proteins/metabolism , Repressor Proteins/genetics , DNA-Binding Proteins/metabolism , DNA-Binding Proteins/genetics , Gene Regulatory Networks
2.
Int J Med Microbiol ; 312(4): 151555, 2022 May.
Article in English | MEDLINE | ID: mdl-35483107

ABSTRACT

Cholera is a life-threatening diarrheal disease caused by the human pathogenic bacterium Vibrio cholerae. Regulatory elements are essential for bacterial transition between the natural aquatic environment and the human host. One of them is the alternative sigma factor RpoS and its anti-sigma factor RssB. Regulation principles seem to be conserved among RpoS/RssB interaction modes between V. cholerae and Enterobacteriaceae species, however the associated input and output pathways seem different. In Escherichia coli, RpoS/RssB is important for the activation of an emergency program to increase persistence and survival. Whereas, it activates motility and chemotaxis in V. cholerae, used strategically to escape from starvation conditions. We characterised a starvation-induced interaction model showing a negative feedback loop between RpoS and RssB expression. We showed by genotypic and phenotypic analysis that rssB influences motility, growth behaviour, colonization fitness, and post-infectious survival. Furthermore, we found that RssB itself is a substrate for proteolysis and a critical Asp mutation was identified and characterised to influence rssB phenotypes and their interaction with RpoS. In summary, we present novel information about the regulatory interaction between RpoS and RssB being active under in vivo colonization conditions and mark an extension to the feedback regulation circuit, showing that RssB is a substrate for proteolysis.


Subject(s)
Escherichia coli Proteins , Vibrio cholerae , Bacterial Proteins/metabolism , DNA-Binding Proteins/genetics , Escherichia coli/metabolism , Escherichia coli Proteins/genetics , Gene Expression Regulation, Bacterial , Sigma Factor/genetics , Sigma Factor/metabolism , Transcription Factors/genetics , Vibrio cholerae/metabolism
3.
Mol Microbiol ; 115(6): 1244-1261, 2021 06.
Article in English | MEDLINE | ID: mdl-33330989

ABSTRACT

Bile resistance is essential for enteric pathogens, as exemplified by Vibrio cholerae, the causative agent of cholera. The outer membrane porin OmpU confers bacterial survival and colonization advantages in the presence of host-derived antimicrobial peptides as well as bile. Expression of ompU is controlled by the virulence regulator ToxR. rpoE knockouts are accompanied by suppressor mutations causing ompU downregulation. Therefore, OmpU constitutes an intersection of the ToxR regulon and the σE -pathway in V. cholerae. To understand the mechanism by which the sigma factor σE regulates OmpU synthesis, we performed transcription studies using ompU reporter fusions and immunoblot analysis. Our data revealed an increase in ompU promoter activity in ΔrpoE strains, as well as in a ΔompU background, indicating a negative feedback regulation circuit of ompU expression. This regulation seems necessary, since elevated lethality rates of ΔrpoE strains occur upon ompU overexpression. Manipulation of OmpU's C-terminal portion revealed its relevance for protein stability and potency of σE release. Furthermore, ΔrpoE strains are still capable of elevating OmpU levels under membrane stress conditions triggered by the bile salt sodium deoxycholate. This study provides new details about the impact of σE on ompU regulation, which is critical to the pathogen's intestinal survival.


Subject(s)
Adhesins, Bacterial/biosynthesis , Bacterial Proteins/metabolism , DNA-Binding Proteins/metabolism , Sigma Factor/genetics , Transcription Factors/metabolism , Vibrio cholerae/genetics , Adhesins, Bacterial/genetics , Bacterial Outer Membrane Proteins/genetics , Bacterial Outer Membrane Proteins/metabolism , Gene Deletion , Gene Expression Regulation, Bacterial/genetics , Porins/biosynthesis , Porins/genetics , Promoter Regions, Genetic/genetics , Vibrio cholerae/metabolism
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