1.
Allergy
; 77(1): 309-313, 2022 01.
Article
in English
| MEDLINE
| ID: mdl-34543440
Subject(s)
Lipoxins , Allergens , Eosinophils , Humans , Inflammation , Killer Cells, Natural , Nasal Mucosa , Nasal Provocation Tests
2.
J Immunol Methods
; 463: 47-53, 2018 12.
Article
in English
| MEDLINE
| ID: mdl-30217720
ABSTRACT
Here we extensively describe a FACS-based protocol for isolating intact non-stained human eosinophils from peripheral blood; a stop forward from our recently published initial study. This method of purification could be accomplished in <3â¯h with only small volumes of whole blood necessary, even in healthy subjects generally exhibiting low levels of circulating eosinophils. Eosinophil activation during the isolation steps appeared to be minimal and this purification procedure yielded high quality RNA. Moreover, these FACS-isolated eosinophils had prolonged viability in culture and were suitable for further activation assays.