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1.
Rev. Cons. Reg. Odontol. Pernamb. ; 4(1): 59-66, jan.-jun. 2001. ilus, tab
Article in Portuguese | BBO - Dentistry | ID: biblio-856281

ABSTRACT

O autor, neste trabalho, tem como objetivo a determinação de um modelo para estimar a idade de indivíduos a partir da área de capeamento epifisário do rádio observada em radiografias de mão e punho esquerdo. As áreas do capeamento epifisário do rádio foram determinadas através da utilização de um aplicativo com base em imagens digitalizadas em computador. Foram utilizadas 55 radiografias de indivíduos do sexo masculino e 55 do sexo feminino, com idade variando entre sete e dezoito anos. Foram obtidos modelos para indivíduos do sexo feminino e sexo masculino. A análise dos dados mostram que o aumento da idade está associada à diminuição da área de capeamento epifisário do rádio. Os resultados deste estudo evidenciam a conveniência e o potencial quanto ao uso de modelos de regressão, na obtenção de estimativas da idade dos indivíduos em função da área de capeamento epifisário do rádio


Subject(s)
Age Determination by Skeleton/methods , Radius , Radiography
2.
Artif Organs ; 25(1): 7-13, 2001 Jan.
Article in English | MEDLINE | ID: mdl-11167553

ABSTRACT

In the last few years, the demand has increased for research on polymeric materials, which can be used as substitutes for injured tissues and organs or to improve their regeneration. In this work, we studied poly(L-lactic acid) (PLLA) membranes, a resorbable biomaterial, which were either dense or had different pore diameters (less than 45 microm, between 180 and 250 microm, and between 250 and 350 microm), in relation to stimulation of cell adhesion, growth, and differentiation in vitro. We used Vero cells, a fibroblastic cell line, as the biological model of investigation. We found that cells attached slowly to all PLLA membranes studied. On the other hand, once the adhesion occurs, the cells are able to grow and differentiate on the different polymers. The cells grew to form a confluent monolayer and were capable of producing collagen Type IV and fibronectin on different PLLA membranes. This behavior indicates that cells try to create a better environment to stimulate their growth. This also indicates that Vero cells alter their differentiation pattern once they are producing extracellular matrix molecules related to epithelial differentiation.


Subject(s)
Cell Differentiation , Fibroblasts/physiology , Lactic Acid , Membranes, Artificial , Polymers , Absorbable Implants , Animals , Biocompatible Materials , Cell Adhesion , Cell Division , Chlorocebus aethiops , Collagen/analysis , Fibroblasts/chemistry , Fibroblasts/cytology , Fibroblasts/ultrastructure , Fibronectins/analysis , Immunohistochemistry , Microscopy, Electron, Scanning , Polyesters , Porosity , Vero Cells
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