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A method to analyze, sort, and retain viability of obligate anaerobic microorganisms from complex microbial communities.
Thompson, Anne W; Crow, Matthew J; Wadey, Brian; Arens, Christina; Turkarslan, Serdar; Stolyar, Sergey; Elliott, Nicholas; Petersen, Timothy W; van den Engh, Ger; Stahl, David A; Baliga, Nitin S.
J Microbiol Methods ; 117: 74-7, 2015 Oct.
Article in English | MEDLINE | ID: mdl-26187776

ABSTRACT

A high speed flow cytometric cell sorter was modified to maintain a controlled anaerobic environment. This technology enabled coupling of the precise high-throughput analytical and cell separation capabilities of flow cytometry to the assessment of cell viability of evolved lineages of obligate anaerobic organisms from cocultures.


Subject(s)
Bacteria, Anaerobic/isolation & purification , Bacteriological Techniques/methods , Flow Cytometry/methods , Microbial Viability , Bacteria, Anaerobic/cytology , Bacteriological Techniques/instrumentation , Equipment Design , Flow Cytometry/instrumentation , Single-Cell Analysis
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