Cranial discrete traits in a Byzantine population and eastern Mediterranean population movements.

Since the beginning of the Holocene, the Anatolian region has been a crossroads for populations and civilizations from Europe, Asia, and the Near to Middle East, with increasing interactions since the Bronze Age. In this context, we examine cranial discrete traits from a Byzantine population from southwest Turkey, excavated at the archeological site of Sagalassos; the site displays human occupation since the 12th millennium B.P. To investigate the biological history of this population, we analyzed the frequency distribution of 17 cranial discrete traits from Sagalassos and 27 Eurasian and African populations. Ward's clustering procedure and multidimensional scaling analyses of the standardized mean measure of divergence (MMD(st)), based on trait frequencies, were used to represent the biological affinity between populations. Our results, considered within a large interpretive framework that takes into account the idea that populations are dynamic entities affected by various influences through time and space, revealed different strata of the Sagalassos biological history. Indeed, beyond an expected biological affinity of the Sagalassos population with eastern Mediterranean populations, we also detected affinities with sub-Saharan and northern and central European populations. We hypothesize that these affinity patterns in the Sagalassos biological package are the traces of the major migratory events that affected southwest Anatolia over the last millennia, as suggested from biological, archeological, and historical data.

Mass spectrometric methods prove the use of beeswax and ruminant fat in late Roman cooking pots.

Lipid extracts of sherds of archaeological late Roman cooking pots were analysed using high temperature-gas chromatography coupled to a mass spectrometer and liquid chromatography with atmospheric pressure chemical ionization mass spectrometer detection (LC-APCI-MS). With these advanced techniques the use of beeswax was shown through identification of the constituting alkanes, mono and diesters. The detection of high amounts of saturated triacylglycerols (TAGs) further indicated that animal fat was processed in these pots. Part of the animal fat was characterised as originating from ruminants due to the presence of trans-fatty acids. The distribution of saturated TAGs and the higher concentration of stearic acid compared to palmitic acid in the transesterified lipid extract indicated that this was sheep fat. The results illustrate how complex mixtures can be unravelled and original contents of ancient ceramic vessels can be determined using specialised analytical equipment.

Modern pollen studies in the territory of Sagalassos (Southwest Turkey) and their use in the interpretation of a Late Holocene pollen diagram.

Modern pollen precipitation studies were carried out on a regional scale in the territory of the classical city of Sagalassos (Southwest Turkey). 57 moss cushions and 7 soil samples from different vegetation types were analysed palynologically. The resulting pollen spectra were grouped into 14 vegetation groups, which were examined using discriminant analysis and principal components analysis. These numerical procedures are applied to display the structure in the data set in a comprehensive way. Some of the modern spectra groups seem to be well-defined, whereas others are difficult to distinguish from each other. After the relationships between the modern pollen spectra had been analysed, the fossil data from a core from the marsh of Gravgaz were added to the data set so that any similarities between fossil and modern spectra could be seen. A joint principal components analysis of the modern and fossil pollen spectra revealed that the pollen spectra from the earliest (ca. 2600-2500 BP; Cal. 830-590 BC till 790-520 BC) and the latest (starting at ca. 1300 BP; Cal. 660-780 AD) pollen assemblage zones have modern counterparts. On the other hand, the pollen spectra from the pollen assemblage zone dominated by Artemisia (ca. 2500-2300 BP; Cal. 790-520 BC till 410-210 BC) and from the pollen assemblage zone that indicated the practice of arboriculture (ca. 2300-1300 BP; Cal. 410-210 BC till 660-780 AD) have no close modern analogues, although individual tabulated pollen percentages revealed some similarities with some modern spectra groups.

Analysis of oil used in late Roman oil lamps with different mass spectrometric techniques revealed the presence of predominantly olive oil together with traces of animal fat.

The lipid fraction of residues in ancient oil lamps found at the archaeological site of Sagalassos (south-west Turkey) was analysed by gas chromatography (GC) coupled to mass spectrometry (MS). The identification of plant sterols and long chain alcohols suggested that a vegetable oil was used in these lamps. The lipid sample was also analysed with reversed-phase liquid chromatography (LC) coupled to MS with atmospheric pressure chemical ionization (APCI). The identification of TAG's detected with LC-APCI-MS showed that predominantly olive oil was used as a fuel for the antique oil lamps. The presence of large quantities of multiply unsaturated triacylglycerol (TAG) and traces of saturated TAG indicated that also other oils and animal fat were added. Summarizing, the analysis of TAG's with LC-APCI-MS in lipid extracts of ancient ceramics proved to be a valuable method to reconstitute the original contents.