Quantitative and qualitative analysis of pyrrolizidine alkaloids in liqueurs, elixirs and herbal juices.

Pyrrolizidine alkaloids (PAs and corresponding N-oxides (PANOs)) are known to have adverse health effects. Their toxic effects on liver cells are especially well-documented. In addition, potential carcinogenic and mutagenic effects in chronic exposure via food and/or herbal medicines have been a subject of vivid discussion in the last decade. Liqueurs and elixirs are traditionally used alcoholic extracts made from parts of plants and herbs. PA cross-contamination of the final products seems likely. Hence, this study aims to detect and quantify the PAs in such products in the light of a possible PA-contamination. The PA content was determined in the form of a single sum parameter using HPLC-ESI-MS/MS and a stable isotope-labeled internal standard. Overall, 56 products available at German pharmacies, drugstores, or internet shops were analyzed, comprising in total 38 samples of liqueurs (mainly bitters), 12 samples of plant elixirs and six different herbal juices. The results showed that 9 out of 38 liqueurs were PA-positive (24%). The total amount of PAs ranged from non-detectable to 9.5 µg/kg. Seven out of ten elixirs were PA-positive (70%) with a maximum PA-content of 3121 µg/kg. Four out of six plant juices were PA-positive (67%) with an average of 4.4 µg/kg (PA-positive samples only).The results and potential risks are discussed in the light of recommended portions for daily consumption or daily doses, in association with the detected PA amounts for individual products and product classes.

What's Next? Michael Crichton's and Mikhail Bulgakov's Criticism of Fetishism in the Life Sciences.

What's Next? Michael Crichtons und Mikhail Bulgakovs Kritik der Fetischisierung in den Lebenswissenschaften. Dieser Beitrag wurde angeregt durch den Thriller Next (2006) von Michael Crichton. Im Gegensatz zu dessen State of Fear (2004), wo die Behandlung eines aktuellen wissenschaftspolitischen Problems - des Klimawandels - mit einer harschen Kritik am Umgang politischer Aktivisten mit wissenschaftlichen Ergebnissen einhergeht, setzt Next Hoffnungen und Ängste ins Zentrum, die im Zusammenhang mit dem 'Human Genome Project' verhandelt wurden. Crichton stellt hier wissenschaftlich-ökonomische Verflechtungen dar, vor denen er schon in seinen Romanen zu Jurassic Park (1990) warnte. Hier wird auf die Gefahr der Fetischisierung im Zusammenhang mit utopisch untermalten wissenschaftlich-technischen Großprojekten und der Phantasie 'Leben zu machen' hingewiesen, und es werden entsprechende Motive und Narrative der ,longue-durée' aufgegriffen, z.B. künstliche Menschen, menschliche Hybris und das Außer-Kontrolle-Geraten wissenschaftlich-technischer Großprojekte. Unter der Fragestellung, wie kritische Wissenschaftsreflexion im Medium von Literatur erfolgen kann und was der spezifische Beitrag aus der Wissenschaftsgeschichte wäre, behandelt dieser Essay neben Werken von Michael Crichton (vor allem Next und Lost World, 1997) auch die satirischen Novellen Die verhängnisvollen Eier (1924/1925) und Hundeherz (1926/1968) von Mikhail Bulgakov, da bereits dort die (mögliche) künstliche Hervorbringung von Lebewesen unter der Bedingung eines (versuchten) direkten Zugriffs auf deren Reproduktionsmechanismen fokussiert wurde. Wissenschaftskritik als Gesellschaftskritik zeigt sich hier als Reflexion auf die Grenzen zwischen Wissenschaft und Gesellschaft und auf die Verantwortung der entlang dieser Grenzen agierenden Menschen, aber auch auf strukturelle Gewalt und deren Auswirkungen auf die Verhältnisse zwischen Menschen und Naturdingen sowie unter Menschen. Summary: What's Next? Michael Crichton's and Mikhail Bulgakov's Criticism of Fetishism in the Life Sciences. This paper was first inspired by Michael Crichton's last thriller, Next (2006), which staged hopes and fears triggered by the completion of the Human Genome Project and by the perfection of Polymerase chain reaction techniques, enabling the replication of DNA on a large scale. These developments nourished fantasies about the artificial (re)construction of living beings from DNA. Crichton had already warned of the fetishization of artificially produced living beings in Jurassic Park and in the novels on which the film was based inventing a futuristic scenario where this was happening on a large scale. Here, the topics of hubris and hybrids were center stage. In Next, the fetishization of life is an effect of the growing encroachment of economic actors upon the life sciences. This paper compares Crichton's criticism of techno-scientific fetishism with Mikhail Bulgakov's critical account of human tinkering with the reproductive organs of humans and non-humans in his two satirical novels The Fateful Eggs and Dog's Heart. The works of both authors link criticism of science with criticism of society. They focus the borders between science and society and analyze the responsibilities of humans who are acting along those borders. The thrillers and satirical novels illustrate the - often violent - power relations between humans and nature and also among humans. Comparing two authors who wrote nearly a century apart from each other and focussing different social systems will help compare longue-durée and more specific forms of techno-scientific fetishism.

[Systems, boundaries and resources: the lexicographer Gerhard Wahrig (1923-1978) and the genesis of his project "dictionary as database"]. / Der Lexikograf Gerhard Wahrig--Systermgrenzen und Ressourcen: Zur Entstehung seines Projekts "Wörterbuch als Datenbank".

Gerhard Wahrig's private archive has recently been retrieved by the authors and their siblings. We undertake a first survey of the unpublished material and concentrate on those aspects of Wahrig's bio-ergography which stand in relation to his life project "dictionary as database", realised shortly before his death. We argue that this project was conceived in the 1950s, while Wahrig was writing and editing dictionaries and encyclopedias for the Bibliographisches Institut in Leipzig. Wahrig, who had been a wireless operator in WWII, was well informed about the development of computers in West Germany. He was influenced both by Ferdinand de Saussure and by the discussion on language and structure in the Soviet Union. When he crossed the German/German border in 1959, he experienced mechanisms of exclusion before he could establish himself in the West as a lexicographer. We argue that the transfer of symbolic and human capital was problematic due to the cultural differences between the two Germanies. In the 1970s, he became a professor of General and Applied Linguistics. The project of a "dictionary as database" was intended both as a basis for extensive empirical research on the semantic structure of natural languages and as a working tool for the average user of the German language. Due to his untimely death, he could not pursue his idea of exploring semantic networks.

Historical and biochemical aspects of a seventeenth century gold-based aurum vitae recipe.

The medicinal chemistry and biomedical applications of gold complexes have been intensively studied over the last decades. Some complexes have been used for the treatment of rheumatoid arthritis, and a considerable number of new metallodrug candidates have been developed as new anticancer drugs and anti-infectives. However, the therapeutic use of gold and its complexes goes back to ancient times and was also of great importance for alchemists until the modern age. In this report, we give an overview of the alchemic medicine between the sixteenth and the early eighteenth century and describe the cytotoxicity and thioredoxin reductase (TrxR) inhibition of a typical "aurum vitae" medicine, which was prepared according to a recipe by Bartholomäus Kretschmar from the seventeenth century. "Aurum vitae" consists of a mixture of gold, mercury and antimony complexes and shows the expected cytotoxic and TrxR inhibitory properties providing some rationale for therapeutic effects of this kind of historical medicinal preparation.

Clocks with hands: instruments, hands and parturients in a changing horizon of time.

This contribution explores the intertwining history of the obstetrician's hand and of (measurable) time. In a long-term historical perspective both on the metaphorical and practical role of the hand in obstetrical discourse and on the changing relation between continuous and discontinuous time, I come to the conclusion that the introduction of certain instruments into obstetrical practice around 1750 went hand in hand with an epistemological change regarding the conception of the living body and its time modes. The obstetrical forceps, which started its career together with male obstetrics and the introduction of the lying-in hospital as a new institution of giving birth, was instrumental in implementing practical and theoretical changes in dealing with the mother's and the child's bodies, which were by no mean beneficial for them.

[A political matter: science and ideology in the 21st century]. / Eine Frage der Politik: Wissenschaft und Ideologie im 21. Jahrhundert.

In the last two decades, history of science and science studies have been quite reluctant to adopt the notion of ideology when analyzing the dynamics of science. This may be an effect of the decreasing popularity of neo-marxist approaches within this disciplinary field; but it is also due to the fact that alternative approaches have been developed, for example Michel Foucault's notion of problematization, Roland Barthes' semiotic mythology, Bruno Latour's re-interpretation of the ontological difference between fact and fetish in science, or Donna Haraway's semi-fictional re-narrations of the techno-scientific world. This contribution undertakes to sketch the impact of two strands of 19th century immanentism on the authors named above, and on their use of concepts related to the notion of ideology, namely fetish, fetishism, myth and mythology respectively. It is argued that in some respect, Marx' concept of commodity fetishism is worth being re-examined, since it articulates a dialectical relation of 'reality' and 'seeming', and its impact on Barthes' mythology is deeper than it might appear at first glance.

["Fabulous things". Drug narratives about coca and cocaine in the 19th century]. / "Fabelhafte Dinge": Arzneimittelnarrative zu Coca und Cocain im 19. Jahrhundert.

This contribution focuses on the history of Coca leaves and Cocaine in the second half of 19th century Europe. Even though, to date, no direct link has been established between the activities of the Milano physician Paolo Mantegazza, and the Göttingen chemist Friedrich Wöhler, it is not a mere coincidence that both published their findings in the same year, namely, 1859. Mantegazza authored the first treatise claiming that Coca had psychoactive qualities and touted its broad therapeutic faculties; he claimed that it should be introduced into European pharmacotherapy. In Wöhler's laboratory, cocaine was isolated from leaves by his pupil Alfred Niemann; later, Wilhelm Lossen refined and corrected Niemann's results. Narratives about medicinal drugs often streamline history into a story that starts with multiple meanings and impure matters and ends with well-defined substances, directed at clear-cut diseases and symptoms. In the case of Coca, however, the pure substance triggered no such process well into the 1880s, whereas the leaves continued to circulate as an exotic, pluripotent drug whose effects where miraculous and yet difficult to establish.