ABSTRACT
OBJECTIVE: The objective of our laboratory study was to determine the bonding efficacy of two-step self-etching primer system (Clearfil SE Bond), all-in-one adhesive (Clearfil Tri S Bond) and acid-etching system (Single Bond) to human fluorosed dentine. METHODS: Forty-eight human molars were grouped according to modified Thylstrup-Fejerskov index (TFI) into normal (N, TFI 0), mild fluorosis (ML, TFI 1-3) and moderate fluorosis (MD, TFI 4-6). Superficial dentine was exposed and ground with 600-grit silicon carbide paper. Teeth of each group were randomly divided into three sub-groups. Each sub-group was treated with one of the three bonding systems. A resin composite was built up on the bonded surfaces and stored in water at 37 degrees C for 24h. They were serially sectioned in buccal-lingual direction into 0.7-1.0mm thick slabs. Slices were trimmed for microtensile bond test and stressed in tension at a crosshead speed of 1mm/min. The data were analyzed with two-way ANOVA (p=0.05). The adhesive/dentine interfaces of the bonded specimens were examined by a FE-SEM. RESULTS: Significantly higher microtensile bond strength (microTBS) was found with Clearfil SE Bond to mild fluorosed dentine than did Single Bond and Clearfil Tri S Bond. Lower microTBS were found for Single Bond (not significant) and Clearfil Tri S Bond than Clearfil SE Bond with moderately fluorosed dentine. Thickness of the hybrid layer produced by Clearfil SE Bond in mild and moderately fluorosed groups was less than with normal dentine. CONCLUSION: Two-step self-etching system, Clearfil SE Bond, showed a higher bonding performance to fluorosed dentine than Single Bond and Tri S Bond.
Subject(s)
Acid Etching, Dental , Dental Bonding/methods , Dentin-Bonding Agents/chemistry , Dentin/pathology , Fluorosis, Dental/pathology , Resin Cements/chemistry , Bisphenol A-Glycidyl Methacrylate/chemistry , Carbon Compounds, Inorganic/chemistry , Composite Resins/chemistry , Humans , Materials Testing , Microscopy, Electron, Scanning , Silicon Compounds/chemistry , Stress, Mechanical , Surface Properties , Temperature , Tensile Strength , Time Factors , Water/chemistryABSTRACT
OBJECTIVE: Objective of our laboratory study was to determine the impact of dental fluorosis severity on the formation of caries in the human enamel and dentine. MATERIALS AND METHODS: Thirty-three human molars were grouped according to modified Thylstrup-Fejerskov index (TFI) into normal (N, TFI 0), mild fluorosis (ML, TFI 1-3) and moderate fluorosis (MD, TFI 4-6). Three mesio-distal sections were made in corono-apical axis of the tooth, giving enamel and dentine samples. They were embedded in an epoxy resin, and polished. Half of the polished surface was covered with an acid resistant varnish and immersed in standard acidified buffer solution (pH 4.5) for 48 h to create artificial caries lesions. They were treated with 5% NaOCl for 45 min and sectioned longitudinally along the center into two halves. Cut surfaces were polished and observed under a confocal laser scanning microscope for depth of demineralization. Morphology of the demineralized zones was observed under a field emission scanning electron microscope (FE-SEM). Data were analyzed using one-way ANOVA and Sheffe test (p=0.05). RESULTS: Statistically significant difference in depth of demineralization was found between N and MD groups (p=0.046) in the enamel, and between N and ML (p=0.002), N and MD (p<0.001), ML and MD (p=0.029) in dentine. FE-SEM observation of the normal enamel showed direct dissolution with large fissures. Spongy appearance of intertubular dentine gradually disappeared from N to MD. CONCLUSIONS: Moderately fluorosed enamel showed a significant caries resistance. In contrast, mild and moderately fluorosed dentine was significantly caries susceptible in vitro.
