ABSTRACT
Cryptosporidium is an enteric parasite of public health significance that causes diarrhoeal illness through faecal oral contamination and via water. Zoonotic transmission is difficult to determine as most species of Cryptosporidium are morphologically identical and can only be differentiated by molecular means. Transmission dynamics of Cryptosporidium in rural populations were investigated through the collection of 196 faecal samples from diarrheic (scouring) calves on 20 farms and 63 faecal samples from humans on 14 of these farms. The overall prevalence of Cryptosporidium in cattle and humans by PCR and sequence analysis of the 18S rRNA was 73.5% (144/196) and 23.8% (15/63), respectively. Three species were identified in cattle; Cryptosporidium parvum, Cryptosporidium bovis and Cryptosporidium ryanae, and from humans, C. parvum and C. bovis. This is only the second report of C. bovis in humans. Subtype analysis at the gp60 locus identified C. parvum subtype IIaA18G3R1 as the most common subtype in calves. Of the seven human C. parvum isolates successfully subtyped, five were IIaA18G3R1, one was IIdA18G2 and one isolate had a mix of IIaA18G3R1 and IIdA19G2. These findings suggest that zoonotic transmission may have occurred but more studies involving extensive sampling of both calves and farm workers are needed for a better understanding of the sources of Cryptosporidium infections in humans from rural areas of Australia.
Subject(s)
Cattle Diseases/transmission , Cryptosporidiosis/transmission , Cryptosporidium/isolation & purification , Feces/parasitology , Zoonoses/transmission , Animals , Base Sequence , Cattle , Cattle Diseases/epidemiology , Cattle Diseases/parasitology , Cryptosporidiosis/epidemiology , Cryptosporidium/classification , Cryptosporidium/genetics , DNA, Ribosomal/chemistry , Genotype , Humans , Molecular Sequence Data , New South Wales/epidemiology , Polymerase Chain Reaction , Prevalence , RNA, Ribosomal, 18S/chemistry , RNA, Ribosomal, 18S/genetics , Risk Factors , Sequence Alignment , Zoonoses/epidemiology , Zoonoses/parasitologyABSTRACT
Hendra virus infection is an emerging infectious disease that is not well understood. Most cases of Hendra virus infection have occurred in Queensland, with one case in a horse in NSW. Hendra virus infection has a high mortality rate in horses and humans and as cases could occur anywhere in Australia it is important to be ready for prompt action should an outbreak occur in NSW. This paper: reviews the current knowledge on Hendra virus infection including methods for preventing the disease; explains the animal health and human health response for an outbreak within NSW; and discusses possible future avenues for post-exposure prophylaxis and prevention by vaccination.
Subject(s)
Communicable Diseases, Emerging/epidemiology , Hendra Virus/isolation & purification , Henipavirus Infections/diagnosis , Henipavirus Infections/prevention & control , Horse Diseases/virology , Animals , Communicable Disease Control/methods , Communicable Diseases, Emerging/virology , Disease Outbreaks , Enzyme-Linked Immunosorbent Assay , Henipavirus Infections/epidemiology , Henipavirus Infections/transmission , Henipavirus Infections/virology , Horse Diseases/epidemiology , Horse Diseases/transmission , Horses , Humans , Polymerase Chain Reaction/methods , Queensland/epidemiology , Zoonoses/transmissionABSTRACT
A multi-agency investigation followed the notification of four locally acquired human brucellosis cases in north-west NSW. Feral pig hunting within a geographically discrete region was identified as the likely exposure with Brucella suis the suspected cause. To test whether feral pigs in the region were infected with Brucella, serological testing was performed on trapped feral pigs and testicular abscesses from condemned carcasses bound for export were cultured. Although no Brucella species were identified in the feral pigs tested in NSW, Leptospira species were. Strengthening of human surveillance and ongoing collaboration between animal and human health agencies is required to confirm that Brucella suis causes brucellosis in humans and feral pigs in north-west NSW.
Subject(s)
Animals, Wild/microbiology , Brucella suis/isolation & purification , Brucellosis/microbiology , Brucellosis/veterinary , Swine Diseases/microbiology , Zoonoses/microbiology , Animals , Humans , New South Wales , Swine , Swine Diseases/transmission , Zoonoses/transmissionABSTRACT
Cryptosporidium hominis, which has an anthroponotic transmission cycle and Cryptosporidium parvum, which is zoonotic, are the primary species of Cryptosporidium that infect humans. The present study identified the species/genotypes and subgenotypes of Cryptosporidium in 7 human and 15 cattle cases of sporadic cryptosporidiosis in rural western NSW during the period from November 2005 to January 2006. The species/genotype of isolates was determined by PCR sequence analysis of the 18S rRNA and C. parvum and C. hominis isolates were subgenotyped by sequence analysis of the GP60 gene. Fourteen of 15 cattle-derived isolates were identified as C. parvum and 1 as a C. bovis/C. parvum mixture. Of the human isolates, 4 were C. parvum and 3 were C. hominis. Two different subgenotypes were identified with the human C. hominis isolates and six different subgenotypes were identified within the C. parvum species from humans and cattle. All four of the C. parvum subtypes found in humans were also found in the cattle, indicating that zoonotic transmission may be an important contributor to sporadic human cases cryptosporidiosis in rural NSW.
