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1.
Vox Sang ; 90(4): 286-93, 2006 May.
Article in English | MEDLINE | ID: mdl-16635071

ABSTRACT

BACKGROUND AND OBJECTIVES: The aim of this study was to evaluate the pharmacokinetics, efficacy and safety of a newly developed 10% liquid immunoglobulin preparation in patients with primary immunodeficiency diseases. This new preparation for intravenous use includes three dedicated virus clearance steps in its manufacturing process to ensure a high margin of viral safety. MATERIALS AND METHODS: This was a prospective, open-label, non-controlled, multicentre study. Twenty-two subjects with primary immunodeficiency were treated initially with three infusions of a licensed intravenous immunoglobulin to standardize the immunoglobulin G (IgG) replacement therapy of all subjects to the same intravenous product. A total of nine infusions of the new 10% liquid preparation were subsequently administered. RESULTS: The median terminal half-life of total IgG following administration of the new preparation was 30.1 days. Median terminal half-lives for IgG subclasses IgG(1), IgG(2), IgG(3) and IgG(4) were 28.3, 31.3, 20.9 and 24.2 days, respectively. The median total serum IgG steady-state trough level was 8.51 g/l. No severe infection episodes started after initiation of treatment with the new preparation. The median rate of mild or moderate infection episodes was 0.48 per month. A total of 194 infusions with the new 10% liquid immunoglobulin preparation were administered. The mean dose per infusion was 0.41 g/kg body weight and the maximum infusion rates recorded were 8 ml/kg/h. Adverse experiences were mostly mild and unrelated to the study drugs. Only 4% of infusions with the new product were followed by one or more related adverse experiences. CONCLUSION: The new 10% liquid immunoglobulin preparation was well tolerated and shown to have an excellent pharmacokinetic, efficacy and safety profile. The liquid formulation provides convenience to patients and healthcare professionals.


Subject(s)
Agammaglobulinemia/therapy , Immunoglobulins, Intravenous/pharmacokinetics , Adult , Agammaglobulinemia/complications , Aged , Drug Tolerance , Female , Half-Life , Humans , Immunoglobulins, Intravenous/adverse effects , Immunoglobulins, Intravenous/therapeutic use , Infection Control , Infections/etiology , Male , Middle Aged , Prospective Studies , Safety
2.
Leukemia ; 17(9): 1827-33, 2003 Sep.
Article in English | MEDLINE | ID: mdl-12970783

ABSTRACT

In this prospective randomized multicenter trial 93 patients, median age 72 years, with RAEB-t (n=25) and myelodysplastic syndrome (MDS)-AML (n=68) were allocated to a standard induction chemotherapy regimen (TAD 2+7) with or without addition of granulocyte-macrophage-CSF (GM-CSF). The overall complete remission (CR) rate was 43% with no difference between the arms. Median survival times for all patients, CR patients, and non-CR patients were 280, 550, and 100 days, respectively, with no difference between the arms. Response rates were significantly better in patients with serum lactate dehydrogenase (S-LDH) levels

Subject(s)
Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Cytarabine/therapeutic use , Daunorubicin/therapeutic use , Granulocyte-Macrophage Colony-Stimulating Factor/therapeutic use , Leukemia, Myeloid/drug therapy , Thioguanine/therapeutic use , Acute Disease , Adult , Aged , Aged, 80 and over , Anemia, Refractory, with Excess of Blasts/drug therapy , Anemia, Refractory, with Excess of Blasts/pathology , Antineoplastic Combined Chemotherapy Protocols/adverse effects , Cell Transformation, Neoplastic , Cytarabine/adverse effects , Daunorubicin/adverse effects , Female , Follow-Up Studies , Granulocyte-Macrophage Colony-Stimulating Factor/administration & dosage , Humans , Leukemia, Myeloid/pathology , Male , Middle Aged , Myelodysplastic Syndromes/drug therapy , Myelodysplastic Syndromes/pathology , Prospective Studies , Remission Induction , Survival Rate , Thioguanine/adverse effects
3.
Stroke ; 32(11): 2567-74, 2001 Nov.
Article in English | MEDLINE | ID: mdl-11692018

ABSTRACT

BACKGROUND AND PURPOSE: Patients treated with oral anticoagulants (ACs) have an increased risk of intracerebral hemorrhage (ICH), which is more often fatal than spontaneous ICH. Options to reverse the AC effect include intravenous administration of vitamin K, plasma, and coagulation factor concentrate. However, the optimal management of AC-related ICH has not been determined in any randomized trial. In this study, the present management of AC-related ICH was surveyed, and determinants of survival were assessed. METHODS: We retrospectively reviewed the medical records of all AC-related ICHs at 10 Swedish hospitals during a 4-year period, 1993 to 1996. Survival status after the ICH was determined from the Swedish National population register. RESULTS: We identified 151 patients with AC-related ICH. Death rates were 53.6% at 30 days, 63.6% at 6 months, and 77.5% at follow-up (mean 3.5 years). The case fatality ratio at 30 days was 96% among patients unconscious on admission (n=27), 80% among patients who became unconscious before active treatment was started (n=15), 55% among patients in whom no special action was taken except withdrawal of AC treatment (n=42), and 28% among patients given active anti-coumarin treatment while they were still conscious (n=64). The case fatality ratio at 30 days was 11% in the group treated with plasma (n=18), 30% in the group treated with vitamin K (n=23), and 39% in the group treated with coagulation factor concentrate (n=23). Within the first 24 to 48 hours after admission, 47% of the patients deteriorated. Choice of therapy to reverse the AC effect differed substantially between the hospitals (P<0.0001), as did the time interval from symptom onset to start of treatment. Multiple logistic regression analysis showed only 2 factors (intraventricular extension of bleeding and ICH volume) that were independently related to case fatality at both 30 days and 6 months. The results were similar when the analysis was restricted to patients who were conscious on admission. CONCLUSIONS: In AC-related ICH, a progressive neurological deterioration during the first 24 to 48 hours after admission is frequent, and the mortality is high. Choice of therapy to reverse the AC effect differed considerably between the hospitals. There was no evidence that any treatment strategy was superior to the others. A randomized controlled trial is needed to determine the best choice of treatment.


Subject(s)
Anticoagulants/adverse effects , Cerebral Hemorrhage/chemically induced , Adult , Aged , Cerebral Hemorrhage/diagnosis , Cerebral Hemorrhage/therapy , Female , Humans , Male , Middle Aged , Prognosis , Retrospective Studies , Sweden , Tomography, X-Ray Computed
4.
Leuk Res ; 25(1): 13-18, 2001 Jan.
Article in English | MEDLINE | ID: mdl-11137555

ABSTRACT

The outcome of continued EPO therapy was studied in 18 responding MDS patients. The EPO dose was reduced in a stepwise fashion to find the lowest possible maintenance dose. Relapses of anemia were associated with either progressive disease or reduction of the administered EPO dose. In the latter group second responses to renewed EPO therapy were readily achieved. Long-term responses were seen in about a third of the patients. Thus, it seems safe to reduce the EPO dose among responding patients. This approach may have advantages both from a medical and a socio-economic perspective.


Subject(s)
Erythropoietin/therapeutic use , Myelodysplastic Syndromes/drug therapy , Aged , Aged, 80 and over , Anemia, Refractory/drug therapy , Dose-Response Relationship, Drug , Erythropoietin/blood , Female , Follow-Up Studies , Humans , Male , Middle Aged , Recombinant Proteins , Survival Rate , Treatment Outcome
5.
Leuk Lymphoma ; 37(5-6): 571-6, 2000 May.
Article in English | MEDLINE | ID: mdl-11042517

ABSTRACT

Trisomy 12 is one of the most frequent chromosomal abnormalities in B-cell chronic lymphocytic leukemia (CLL), and is predominantly found in CLL with atypical morphology (aCLL). It has been suggested that the atypical morphology might be a feature of the abnormal trisomy 12 clone, but so far it has been difficult to allocate chromosomal aberrations to individual leukemic cells identified by cytomorphology. We therefore wanted to use our MGG/FISH method, which combines fluorescence in situ hybridization (FISH) and standard cytomorphology, to study if the trisomy 12 clone in CLL was restricted to lymphocytes with atypical morphology. Peripheral blood specimens of four patients with aCLL were studied using a DNA probe against the pericentromeric region of chromosome 12. Trisomy 12 was found in 10-34 % of the lymphocytes. In three patients, the proportion of atypical and typical lymphocytes with trisomy 12 was quite comparable, and so was the percentage of atypical cells with lymphoplasmacytoid morphology and those with cleaved nucleus showing trisomy 12. Only one patient differed, since we found an overrepresentation of trisomy 12 among the atypical lymphocytes. However, this could be fully explained by the diluting effect of contaminating T-cells after chemotherapy. The results of the present study show that despite the strong association of trisomy 12 and atypical morphology in CLL, this chromosomal abnormality is not confined to lymphocytes with atypical morphology, but is also found in typical CLL cells. This supports that both cell types have the same clonal origin and that different cell morphology cannot be explained alone by the acquisition of an additional chromosome 12.


Subject(s)
B-Lymphocytes/pathology , Chromosomes, Human, Pair 12 , In Situ Hybridization, Fluorescence , Leukemia, Lymphocytic, Chronic, B-Cell/pathology , Staining and Labeling/methods , Trisomy , Aged , Aged, 80 and over , Cell Nucleus/ultrastructure , Coloring Agents , Eosine Yellowish-(YS) , Female , Humans , Immunophenotyping , Leukemia, Lymphocytic, Chronic, B-Cell/genetics , Male , Methylene Blue , Middle Aged , Neoplastic Stem Cells/pathology
6.
Bone Marrow Transplant ; 24(7): 741-5, 1999 Oct.
Article in English | MEDLINE | ID: mdl-10516677

ABSTRACT

Twenty-five patients with multiple myeloma received bone marrow grafts (n = 24) or peripheral blood stem cells (n = 1) from twin donors. The outcome was compared in a case-matched analysis to 125 patients who underwent autologous transplantation, and 125 who underwent allogeneic transplantation. Seventeen patients (68%) receiving twin transplants entered complete remission, which was not significantly different from that of autologous (48%) or allogeneic (58%) transplants. The median overall and progression-free survival for the twins was 73 and 72 months, respectively. The overall survival tended to be better (73 vs 44 months) and the progression-free survival was significantly better (72 vs 25 months) than with autologous transplantation and both were significantly better than with allogeneic transplantation. Three of 17 patients who entered complete remission following transplantation had relapsed at follow-up. This relapse rate was significantly lower than following autologous transplantation and similar to the relapse rate with allogeneic transplantation. Only two twins died of transplant-related toxicity. Six further patients died of progressive or relapsing disease. Syngeneic transplantation in multiple myeloma appears to be the treatment of choice if a twin donor is available. A lower relapse risk than in autotransplantation may be due to reinfusion of malignant cells in some patients treated with this modality or to the presence of a graft-versus-myeloma effect in some syngeneic transplants.


Subject(s)
Multiple Myeloma/therapy , Transplantation, Isogeneic , Adult , Bone Marrow Transplantation/methods , Case-Control Studies , Diseases in Twins , Female , Hematopoietic Stem Cell Transplantation/methods , Humans , Male , Middle Aged , Survival Rate , Transplantation, Autologous , Transplantation, Homologous
7.
Vox Sang ; 73(4): 212-9, 1997.
Article in English | MEDLINE | ID: mdl-9407638

ABSTRACT

BACKGROUND: Red blood cells stored as concentrates or suspensions in additive solutions change rapidly their oxygen affinity mainly due to the loss of 2,3-diphosphoglycerate (2,3-DPG). When collected in CPD with half of the normal concentration of citrate and citric acid (0.5CPD) and stored in a new additive solution (Erythro-Sol), 2,3-DPG is better maintained. No studies of the oxygen affinity of red cells stored under these conditions have been published. In Erythro-Sol, red cells have a satisfactory in vivo recovery for 49 days but the conditions after 28 days, within which time most red cell units are transfused, have not been investigated. Of importance is also to be able to make platelet concentrates (PCs) from 0.5CPD blood. Little data are available concerning the clinical usefulness of platelets prepared from 0.5CPD buffy coats (BCs). METHODS: Blood was collected in 0.5CPD, held at 20 degrees C for 3-4 h, then separated with the bottom-and-top technique into red cells, plasma and BC. In a storage experiment with 6 U the 2,3-DPG and P50 values were determined weekly and a number of in vitro parameters were tested on day 28. In 6 donors the in vivo recovery and survival of red cells were determined using a single-chromium technique. Transfusions of 212 0.5CPD-Erythro-Sol red cell units were given to hematological patients under supervision. PCs derived from pools of 0.5CPD BCs suspended in PAS2 (T-Sol) were transfused to 20 thrombocytopenic patients and compared with CPD-BC-PCs suspended in PASI. Corrected count increments (CCI) were determined. RESULTS: The erythrocyte 2,3-DPG and P50 values were normal or slightly subnormal initially but increased to supernormal levels during the 1 week, and remained at these levels for a further 1-3 weeks; the 2,3-DPG was two thirds of normal after 28 days, the P50 was 3.72 +/- 0.28 kPa after 14 days and 2.84 +/- 0.41 after 28 days (mean +/- SD). The P50 values corresponded closely (r2 = 0.903) to 2,3-DPG. The in vivo recovery of 4-week-stored red cells was 89.6 +/- 5.5% and the T50 was 32.2 +/- 2.0 days. No adverse effects were observed in the transfusions. The CCI values did not differ between test and control groups; in both, 3- to 5-day-stored PCs gave lower CCI than fresh (0-2 days) PCs. Patients with acute myeloid leukemia AML (n = 11) had significantly lower CCI values than patients with myelodysplastic syndrome, myeloma and lymphoma (n = 9; CCI 1 h: p = 0.001; CCI 24 h: p = 0.006). CONCLUSIONS: Red cells stored in Erythro-Sol sustain a normal or slightly lowered oxygen affinity for 2-4 weeks, their viability is excellent, and they are well tolerated in clinical transfusions. Platelets prepared from 0.5CPD-BCs cause CCI, of the same magnitude as CPD-BCs.


Subject(s)
Blood Platelets/drug effects , Blood Preservation/methods , Erythrocyte Transfusion/methods , Erythrocytes/drug effects , Isotonic Solutions , Platelet Transfusion/methods , Anticoagulants , Citric Acid , Clinical Laboratory Techniques , Humans , Quality Assurance, Health Care
8.
Leuk Res ; 20(8): 693-9, 1996 Aug.
Article in English | MEDLINE | ID: mdl-8913323

ABSTRACT

In order to reduce anaemia in patients with myelodysplastic syndromes (MDS) a stepwise treatment protocol including erythropoietin (EP) and granulocyte-macrophage colony-stimulating factor (GM-CSF) was designed. Thirty-seven MDS patients (stages I-III) with symptomatic anaemia were first given EPO 10,000 U s.c. 3 times weekly for 6 weeks. Those not responding, i.e. increased their haemoglobin levels > 15 g/l, proceeded into the second phase of the study where GM-CSF (200 micrograms/d. s.c. on weeks 1-6) was combined with EPO (10,000 U s.c. 3 times weekly on weeks 5-14). Following the initial EPO treatment phase, 14 of the 37 patients (38%) responded with increased haemoglobin levels. Responders were significantly different from non-responders in that their pre-treatment values of s-EPO, s-LDH and bone marrow blast cell counts were lower, their baseline haemoglobin levels higher and their transfusion dependency less pronounced. Eighteen of the 23 non-responders proceeded into the second phase, 13 of those were evaluable having completed the entire schedule. Three of the 13 initially EPO resistant patients (23%) responded to the GM-CSF/EPO combination with increased haemoglobin levels, suggesting a positive synergy between the two cytokines. Thus, the overall response rate to the present protocol was 46% (17 of 37 cases), but only a limited subset of the patients did clearly benefit from the combined GM-CSF/EPO administration. Therefore, we believe this step-wise approach to multiple growth factor treatment in MDS, starting with EPO alone and reserving the combination for refractory cases, has considerable advantages, taking into account both medical and socio-economical aspects.


Subject(s)
Anemia, Refractory/therapy , Erythropoietin/administration & dosage , Granulocyte-Macrophage Colony-Stimulating Factor/administration & dosage , Aged , Aged, 80 and over , Anemia, Refractory, with Excess of Blasts/therapy , Anemia, Sideroblastic/therapy , Drug Administration Schedule , Drug Synergism , Drug Therapy, Combination , Erythropoietin/adverse effects , Female , Granulocyte-Macrophage Colony-Stimulating Factor/adverse effects , Humans , Male , Middle Aged
9.
Leukemia ; 7(9): 1324-7, 1993 Sep.
Article in English | MEDLINE | ID: mdl-8371582

ABSTRACT

The effect of human recombinant erythropoietin (rhEPO) was investigated in 29 anemic patients with myelodysplastic syndromes (MDS). A rhEPO dosage of 150 U/kg was administered subcutaneously three times weekly for a minimum of 6 weeks. Seven out of 27 evaluable patients (26%) had an effective clinical response to therapy by increasing hemoglobin concentrations by more than 15 g/l (reaching at least 105 g/l) or by eliminating transfusion requirements. Six out of the seven patients responded within four weeks. Three of the responders successfully continued rhEPO treatment 15 months or more. To determine whether it may be possible to predict response to rhEPO, various clinical parameters were examined. Responders were found to be significantly different from non-responders in five aspects: They had less elevated baseline serum EPO levels (92 +/- 33 versus 515 +/- 108 U/l, mean +/- SEM; p = 0.023) and were more often transfusion-independent (71% versus 20% of non-responders; p = 0.022). Furthermore, responders were more often females (71% versus 40% in the non-responding group; p = 0.025), of subtype RA rather than RAEB (four patients and one patient, respectively, compared to seven and nine patients in the non-responding group; p = 0.025), and they predominantly displayed normal karyotypes or a 5q- aberration (86% versus 47%; p = 0.005). We conclude, that rhEPO treatment can reduce anemia in MDS and that certain pre-treatment clinical parameters may be used to predict response.


Subject(s)
Erythropoietin/therapeutic use , Myelodysplastic Syndromes/therapy , Aged , Aged, 80 and over , Anemia, Refractory/therapy , Blood Transfusion , Chromosome Deletion , Chromosomes, Human, Pair 5 , Erythropoietin/blood , Female , Humans , Male , Middle Aged , Myelodysplastic Syndromes/blood , Myelodysplastic Syndromes/genetics , Predictive Value of Tests , Prognosis , Recombinant Proteins/therapeutic use , Sex Factors
10.
Thromb Res ; 70(3): 255-64, 1993 May 01.
Article in English | MEDLINE | ID: mdl-8327990

ABSTRACT

Platelet concentrates (PC) were prepared by platelet apheresis to obtain a low (< 0.1 x 10(9)/l, PC A) and high (2-9 x 10(9)/l, PC B) concentration of white cells from the same donor. The mean platelet count (+/- SD) of the PC were 1000 +/- 50 x 10(9)/l. During storage 71-96% of the white cells were mononuclear. A portion of red cells from each donor was also stored under the same conditions. The results of the PC were corrected for the influence of contaminating erythrocytes. During the whole storage period pO2 was significantly lower in PC with high white cell count, p < 0.05 and the concentration of ATP significantly higher, p < 0.01 on day 0, 3 and 5, p < 0.05 on day 1 and 7. There were no significant differences in pH, pCO2, production of lactate or consumption of glucose. In another 17 apheresis PC leukocyte viability was assessed by exclusion of 0.05% trypan blue on days 5 and 7; 90 +/- 3 and 88 +/- 4% respectively of the leukocytes remained viable; 87 and 96% respectively were mononuclear cells. In a third series of PC prepared with extra high white cell count (0.4-1.6 x 10(9)/unit) was oxidative phosphorylation maintained and pH remained between 6.80 and 7.16 in 12 of 16 PC after 5 days of storage. The presence of mononuclear cells in PC does not impair the maintenance of platelet ATP production and oxidative phosphorylation unless the platelet concentration is high enough almost to exceed the oxygen diffusion capacity of the bag. If so, the white cells significantly contributed to the oxygen deficit and pH and ATP rapidly decreased due to anaerobic metabolism. We conclude that mononuclear cells can be stored in plasma together with platelets at aerobic conditions for 7 days with maintained concentration of platelet ATP.


Subject(s)
Blood Platelets , Blood Preservation , Leukocytes, Mononuclear/metabolism , Adenosine Triphosphate/blood , Blood Glucose/metabolism , Carbon Dioxide/blood , Cell Survival , Humans , Hydrogen-Ion Concentration , Lactates/biosynthesis , Lactic Acid , Oxidative Phosphorylation , Oxygen/blood , Platelet Count , Plateletpheresis , Time Factors
11.
Transfus Med ; 2(2): 135-42, 1992 Jun.
Article in English | MEDLINE | ID: mdl-1308210

ABSTRACT

Proteolytic activity was studied in platelet concentrates (PC) stored in plasma at 22 degrees C. In experiment 1, two PC with a higher (A) and a lower (B) white cell concentration were prepared from each of nine donors by centrifugation. Aliquots of the cell-free plasma, PPP, were stored as a control. Samples for the assay of fibrinopeptide A (FPA), elastase, spontaneous proteolytic activity (SPA), kallikrein-inhibiting activity, thrombin-antithrombin complexes (TAT) and D-dimers were collected initially and on days 1, 3, 5 and 7 of storage. Consumption of glucose, pH and concentrations of lactate dehydrogenase (LDH) and ATP were determined to investigate the metabolic status of the PC. The decrease in pH correlated to the leucocyte count, r = -0.74, P < 0.001 and to the increase in LDH, r = -0.74, P < 0.01. The levels of elastase and the SPA were consistently low in the PPP bags. In the PC elastase had increased by day 5 and the SPA by day 3; the levels in PC A were significantly higher than in PC B, P < 0.01. The leucocyte count correlated with the elastase activity, r = 0.71, P < 0.01, and with the SPA, r = 0.65, P < 0.01. A minor increase in FPA was demonstrated while no TAT and D-dimers could be detected. The cause of the formation of FPA was studied in experiment 2; three bags of PC and four of PPP were prepared from each of 16 donors. To the PC and three of the PPP bags either hirudin, aprotinin or no enzyme inhibitor (control) was added.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Blood Platelets/metabolism , Blood Preservation , Endopeptidases/blood , Plasma , Antithrombin III/analysis , Blood Platelets/drug effects , Chromogenic Compounds/metabolism , Energy Metabolism , Enzyme Activation , Fibrin Fibrinogen Degradation Products/analysis , Fibrinopeptide A/analysis , Humans , Hydrogen-Ion Concentration , Kallikreins/antagonists & inhibitors , Pancreatic Elastase/blood , Peptide Hydrolases/analysis , Temperature
12.
Transfusion ; 30(7): 639-43, 1990 Sep.
Article in English | MEDLINE | ID: mdl-2119532

ABSTRACT

Platelet concentrates (PCs), prepared by plateletpheresis, were stored in aliquots in polyvinylchloride blood bags for 5 days at 22 degrees C under rapid, slow, or no agitation. Nonagitated PCs were also stored in a 98-percent oxygen atmosphere. In nonagitated PCs, pO2, lactate production, and platelet factor 4 (PF 4) concentration increased, whereas the ATP level and pH dropped rapidly. These changes were somewhat minimized in nonagitated PCs stored in oxygen. There was no significant difference between the two agitated groups. The increase in PF 4 correlated inversely to the decrease in ATP: r = -0.91, p less than 0.001, n = 24. The formation of thromboxane B2 (TxB2) after stimulation with arachidonic acid or collagen was significantly higher in slowly agitated PCs on Day 5 than on Day 0 (p less than 0.01). Nonagitated PCs produced lower levels of TxB2 (collagen stimulation) on Day 5 (p less than 0.05). In unstimulated PCs, the levels of TxB2 and ATP were inversely correlated on Day 5 (r = -0.70, p less than 0.001, n = 20). In vivo survival was performed after 72 hours of storage; mean survival (+/- SD) was 6.5 (+/- 0.3) days for nonagitated oxygenated PCs and 6.8 (+/- 0.7) days for agitated PCs. In nonagitated PCs, anaerobic metabolism increased, although oxygen diffusion through the container wall was sufficient. Agitation seems to facilitate the diffusion of oxygen through the storage medium. Nonagitated PCs were stored safely for 24 hours; this period can be extended to at least 72 hours when aerobic metabolism is maintained.


Subject(s)
Blood Platelets/metabolism , Cytoplasmic Granules/metabolism , Motion , Thromboxane B2/metabolism , Blood Cells/cytology , Blood Platelets/analysis , Blood Platelets/cytology , Blood Transfusion , Carbon Dioxide/analysis , Cell Survival/physiology , Humans , Hydrogen-Ion Concentration , Lactates/analysis , Oxygen/analysis , Plateletpheresis
13.
Vox Sang ; 58(1): 40-4, 1990.
Article in English | MEDLINE | ID: mdl-2316209

ABSTRACT

Platelet concentrates (PC), prepared by platelet apheresis, were stored in four different types of blood bags. One of the bags, manufactured with a thinner PVC film than previously, was tested in three different bag volumes. From 25 donors a total number of 99 PC were prepared. Platelet numbers varied from 20 to 140 X 10(9) platelets per bag. The cell count, pH, pO2, pCO2 and lactate were determined initially and on days 1, 3 and 5 of storage. In a separate test, the oxygen diffusion capacity of the bags was determined by oxidation of sodium sulfite in the presence of cobaltous chloride. The oxygen diffusion capacity found was 16 (PL 732, 300 ml), 13.5 (Teruflexa 800 ml), 11.5 (PL 1240, 400 ml), 10.6 (Teruflexa 600 ml), 9 (Teruflexa 400 ml) and 4 (PL 146, 300 ml) mumol O2/h, respectively. For each bag type, the minimum and maximum platelet number stored with maintained pH levels (6.9-7.4) was defined. The maximum platelet number stored with maintained aerobic metabolism, correlated to the oxygen diffusion capacity of the bag, r = 0.998, p less than 0.001, n = 6; thus the maximum platelet number successfully stored for 5 days in each container can be predicted by determination of the oxygen diffusion capacity. In PC with a low platelet yield, pH values above 7.4 were observed after 1 and 3 days. When the results are compared with platelet yield data from routine blood banking, the optimal bags for platelet storage can be chosen. These conclusions must be further investigated in studies in vivo.


Subject(s)
Blood Platelets , Blood Preservation/instrumentation , Plastics , Diffusion , Evaluation Studies as Topic , Hydrogen-Ion Concentration , Oxygen , Platelet Count
14.
Vox Sang ; 55(4): 211-7, 1988.
Article in English | MEDLINE | ID: mdl-3064416

ABSTRACT

A new, automated technique for the preparation of blood components is described. A system of 3 or 4 integrally connected plastic containers (Optipac) is handled by a new type of extractor (Optipress). The container in which the blood is collected has an outlet at the top and another at the bottom. After normal centrifugation to obtain separation of the blood components, these are squeezed out from the top and bottom simultaneously under control of a photocell. The primary separation step results in three components: a leukocyte-poor red-cell suspension in SAGM medium, CPD plasma, and a buffy-coat preparation. The system has been tested in two laboratories (lab A and lab B). A 'heavy-spin' centrifugation to obtain a maximum yield of cell-poor plasma gave the best removal of leukocytes from the red cells; the remaining leukocyte content was 0.46 +/- 0.25 (lab A) and 0.5 +/- 0.4 (lab B) x 10(9)/red-cell unit. Platelet concentrates can be prepared either the normal way via platelet-rich plasma or from buffy coat. Red-cell 24-hour autologous posttransfusion survival using labeling with 51Cr was 87.5 +/- 4.1% (lab A) after 35 days, and 84.2 +/- 4.2% (lab A) and 77.5 +/- 1.5% (lab B) after 42 days. Red-cell morphology and fluidity compared favorably to previous studies using the same additive solution in traditional plastic-bag systems. The total adenine nucleotide concentration was maintained normal for 42 days.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Blood Preservation/methods , 2,3-Diphosphoglycerate , Adenosine Monophosphate/analysis , Blood Cell Count , Blood Glucose/metabolism , Diphosphoglyceric Acids/analysis , Erythrocyte Aging , Erythrocytes , Fibrinopeptide A/metabolism , Hemolysis , Humans , Hydrogen-Ion Concentration , Kallikreins/metabolism , Peptide Hydrolases/metabolism , Plasma , Transfusion Reaction
15.
Vox Sang ; 45(4): 303-11, 1983.
Article in English | MEDLINE | ID: mdl-6415919

ABSTRACT

Platelet concentrates prepared by platelet apheresis were stored in plastic blood bags with different gas permeability properties. Inadequate oxygen supply gave an insufficient adenosine triphosphate (ATP) regeneration and a compensatory increase in glycolysis and lactic acid production, giving a rapidly falling pH. At pH below 6.0 the glycolysis was inhibited, oxygen consumption ceased, and ATP dropped towards depletion. Adequate oxygen supply kept the lactic acid production low with small pH changes only, and allowed a sufficient ATP regeneration. The release of alpha-granular platelet Factor 4 (PF4) was almost total at pH below 6.0, while at intact metabolic function there was a slow release of PF4. Platelet preservation is enhanced by the use of blood bags with adequate gas exchange properties. In our study one polyvinyl chloride plastic (PVC) bag gave poor results, while another PVC bag and a polyolefin bag showed intact metabolism for 5 days and a moderate release of PF4.


Subject(s)
Blood Platelets , Blood Preservation , Adenosine Triphosphate/blood , Blood Glucose/metabolism , Blood Platelets/metabolism , Carbon Dioxide/blood , Humans , In Vitro Techniques , Lactates/blood , Oxygen Consumption , Plastics , Platelet Factor 4/analysis
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