Ancient genomes reveal over two thousand years of dingo population structure.

Dingoes are culturally and ecologically important free-living canids whose ancestors arrived in Australia over 3,000 B.P., likely transported by seafaring people. However, the early history of dingoes in Australia-including the number of founding populations and their routes of introduction-remains uncertain. This uncertainty arises partly from the complex and poorly understood relationship between modern dingoes and New Guinea singing dogs, and suspicions that post-Colonial hybridization has introduced recent domestic dog ancestry into the genomes of many wild dingo populations. In this study, we analyzed genome-wide data from nine ancient dingo specimens ranging in age from 400 to 2,746 y old, predating the introduction of domestic dogs to Australia by European colonists. We uncovered evidence that the continent-wide population structure observed in modern dingo populations had already emerged several thousand years ago. We also detected excess allele sharing between New Guinea singing dogs and ancient dingoes from coastal New South Wales (NSW) compared to ancient dingoes from southern Australia, irrespective of any post-Colonial hybrid ancestry in the genomes of modern individuals. Our results are consistent with several demographic scenarios, including a scenario where the ancestry of dingoes from the east coast of Australia results from at least two waves of migration from source populations with varying affinities to New Guinea singing dogs. We also contribute to the growing body of evidence that modern dingoes derive little genomic ancestry from post-Colonial hybridization with other domestic dog lineages, instead descending primarily from ancient canids introduced to Sahul thousands of years ago.

An examination of the cross-sectional geometrical properties of the long bone diaphyses of Holocene foragers from Roonka, South Australia.

OBJECTIVES: This study examines long bone diaphyseal rigidity and shape of hunter-gatherers at Roonka to make inferences about subsistence strategies and mobility of inhabitants of semi-arid southeastern Australia. Roonka is a cemetery site adjacent to the Lower Murray River, which contains over 200 individuals buried throughout the Holocene. Archaeological evidence indicates that populations living near this river corridor employed mobile, risk averse foraging strategies. METHODS: This prediction of lifestyle was tested by comparing the cross-sectional geometric properties of the humerus, radius, ulna, femur, tibia, and fibula of individuals from Roonka to samples of varying subsistence strategies. Bilateral asymmetry of the upper limb bones was also examined. RESULTS: Roonka males and females have moderately high lower limb diaphyseal rigidity and shape. In the upper limb, females have low rigidity and bilateral asymmetry while males have moderately high rigidity and bilateral asymmetry. This pattern is similar to other foraging groups from Australia and southern Africa that have behaviorally adapted to arid and semi-arid environments. DISCUSSION: Lower limb results suggest that populations in the Lower Murray River Valley had relatively elevated foraging mobility. Upper limb rigidity and bilateral asymmetry indicate a sexual division of labor at Roonka. Females resemble other samples that had mixed subsistence strategies that involved hunting, gathering, and processing tasks. Males display a pattern similar to groups that preferentially hunted large game, but that supplemented this source with smaller game and riverine resources.

Scientific evidence for the identification of an Aboriginal massacre at the Sturt Creek sites on the Kimberley frontier of north-western Australia.

Archival research into episodes of frontier violence in the Kimberley region of Western Australia indicate that the bodies of Aboriginal victims of massacres were frequently incinerated following the event. This paper presents the results of a scientific investigation of a reported massacre at Sturt Creek where burnt bone fragments were identified in two adjacent sites and documents the archaeological signatures associated with the sites. The methodology used to undertake the project brought together three systems of knowledge: the oral testimonies of the descent group originating from a sole adult survivor of the massacre; archival, historical and scientific research. An archaeological survey defined the two distinct sites containing hundreds of fragile bone fragments; a third site was found to be highly disturbed. Scientific investigations included macroscopic and microscopic examination of selected bone fragments by an anatomical pathologist and a zooarchaeologist and X-ray diffraction analysis of sixteen bone fragments. The anatomical pathologist and zooarchaeologist undertook macroscopic and microscopic examinations of selected bone samples to identify morphological evidence for human origin. It was concluded that three bone fragments examined may have been human, and two of the fragments may have been from the vault of a skull. It was concluded that the likelihood of them being human would be strengthened if it was found that the three samples had been subjected to high temperatures. X-ray diffraction analysis of 16 bone fragments provided this evidence. All fragments showed sharp hydroxylapatite peaks (crystallite sizes 9882nm and 597nm respectively) and all had been subjected to extreme temperatures of either 600°C for more than 80h, 650°C for more than 20h, 700°C for more than 4h or 800°C for more than 1h. XRD analyses were also done on bone samples collected from three cooking hearths at three different archaeological sites. It was found that two of the three samples had been exposed to substantially lower temperatures for a short time period. It was concluded that there was strong pathological and archaeological evidence that the bone fragments were human in origin, but that the evidence was not conclusive. This research also identified archaeological signatures for the identification of massacre sites in similar Australian environments and circumstances.

Aboriginal mitogenomes reveal 50,000 years of regionalism in Australia.

Aboriginal Australians represent one of the longest continuous cultural complexes known. Archaeological evidence indicates that Australia and New Guinea were initially settled approximately 50 thousand years ago (ka); however, little is known about the processes underlying the enormous linguistic and phenotypic diversity within Australia. Here we report 111 mitochondrial genomes (mitogenomes) from historical Aboriginal Australian hair samples, whose origins enable us to reconstruct Australian phylogeographic history before European settlement. Marked geographic patterns and deep splits across the major mitochondrial haplogroups imply that the settlement of Australia comprised a single, rapid migration along the east and west coasts that reached southern Australia by 49-45 ka. After continent-wide colonization, strong regional patterns developed and these have survived despite substantial climatic and cultural change during the late Pleistocene and Holocene epochs. Remarkably, we find evidence for the continuous presence of populations in discrete geographic areas dating back to around 50 ka, in agreement with the notable Aboriginal Australian cultural attachment to their country.

Healed Depressed Parasagittal Skull Fractures-A Feature of Archaic Australian Aboriginal Remains.

The skeletal remains of eight Australian Aboriginals with healed depressed skull fractures were examined. Male:female ratio 5:3; age range 20-60 yrs. Burial dates by 14 C dating in three cases were 500 years BP (n = 2) and 1300 BP. There were 13 healed depressed skull fractures manifested by shallow indentations of cortical bone and thinning of diploe, with no significant disturbance of the inner skull tables. Nine (69%) were located within 35 mm of the sagittal suture/midline. These lesions represent another acquired feature that might be helpful in suggesting that a skull is from a tribal Aboriginal individual and may be particularly useful if the remains are represented by only fragments of calvarium. While obviously not a finding specific to this population, these healed injuries would be consistent with the possible results of certain types of conflict behavior reported in traditional Aboriginal groups that involved formalized inflicted blunt head trauma.

Risk Minimization and a Late Holocene Increase in Mobility at Roonka Flat, South Australia: An Analysis of Lower Limb Bone Diaphyseal Shape.

OBJECTIVES: The strengthening of the El Niño Southern Oscillation (ENSO) in the mid-Holocene caused significant changes in climate, vegetation, and faunal assemblages in South Australia. The appearance of a light, flexible backed-artifact toolkit ∼4 kya has been interpreted as evidence for changes in foraging behavior in response to this event. Optimal foraging theory supports a risk minimization strategy for South Australian hunter-gatherers in which increased mobility was used to cope with effects of a dryer, unstable environment in the late Holocene. Whether this event caused changes in foraging mobility will be tested by examining lower limb external diaphyseal shape between pre-ENSO and post-ENSO skeletons from Roonka Flat, South Australia. MATERIALS AND METHODS: Anteroposterior and mediolateral diameters were used to construct diaphyseal shape indices for Roonka Flat femora and tibiae. If populations living in South Australia became more mobile over time, then post-ENSO skeletons should exhibit higher shape indices. RESULTS: The pooled-sex post-ENSO sample has significantly higher femoral shape indices than the pre-ENSO sample. Males do not show significant diaphyseal shape differences over time, but females significantly increase. DISCUSSION: These data are consistent with the risk minimization model, indicating that South Australians became more mobile post-ENSO to better exploit a less productive environment by expanding their foraging radii. The temporal shift toward more elliptical diaphyses is more notable in females than males, which is consistent with Aboriginal ethnographies that show both sexes being intensely involved in hunting and capturing game animals. Am J Phys Anthropol 161:94-103, 2016. © 2016 Wiley Periodicals, Inc.

Novel application of X-ray fluorescence microscopy (XFM) for the non-destructive micro-elemental analysis of natural mineral pigments on Aboriginal Australian objects.

This manuscript presents the first non-destructive synchrotron micro-X-ray fluorescence study of natural mineral pigments on Aboriginal Australian objects. Our results demonstrate the advantage of XFM (X-ray fluorescence microscopy) of Aboriginal Australian objects for optimum sensitivity, elemental analysis, micron-resolution mapping of pigment areas and the method also has the advantage of being non-destructive to the cultural heritage objects. Estimates of pigment thickness can be calculated. In addition, based on the elemental maps of the pigments, further conclusions can be drawn on the composition and mixtures and uses of natural mineral pigments and whether the objects were made using traditional or modern methods and materials. This manuscript highlights the results of this first application of XFM to investigate complex mineral pigments used on Aboriginal Australian objects.

Patterns in ritual tooth avulsion at Roonka.

Tooth avulsion is the intentional removal of one or more teeth for ritual or aesthetic reasons, or to denote group affiliation. Typically the maxillary incisors are the teeth most often selected for removal. Previous authors have discussed the presence of tooth avulsions in several individuals recovered from Roonka, but those papers did not examine any patterns in those removals that might be present. Analysis of the tooth avulsions at Roonka reveals a change in the practice over time, with the older burials from phase II typically showing removal of both maxillary central incisors with a left side bias when only one tooth is removed, and the more recent phase III burials showing only one incisor avulsed and a right side bias for removal. Frequencies in the practice also changed over time, with avulsions being much more common in the older phase II burials. Historical evidence suggests that any particular regional or social group would have its own particular pattern of tooth avulsion, so these changes in tooth avulsions at Roonka suggest that the site was either used by multiple groups of people for burials, or that there was significant cultural change during the occupation of the site.

Hunter-gatherer variability: Dental wear in South Australia.

Often it is assumed that hunter-gatherer dentitions are dominated by heavy attrition. Recent analyses, however, have shown unexpected variability in the pattern of wear between groups. It had been previously noted that wear differed between neighboring groups on the Murray River, Australia. This analysis extends that geographic scope as well as focusing on wear across the dentition, including the premolars. The samples came from coastal and riverine regions of southern Australia. The analysis used records from the Yorke Peninsula, Adelaide Plains (Gillman site), and Euston regions. These were compared with previously published work from the Adelaide Plains and four locations on the Murray River. The results confirm the overall severity of wear but reveal systematic differences between the samples in terms of the pattern of wear. Heavy wear on the incisors and canines is observed among males from the Euston, Kaurna, Middle A, Murray Mouth, and Yorke Peninsula samples but with marked intra-individual variability. Extensive premolar wear is noted among females from Kaurna and Middle B samples as well as among males and females from Euston. It is argued that these patterns relate to gendered non-masticatory use of teeth and reliance upon bulrush (Typha spp.) and related species for both food and fiber among some groups. We argue that analyzing the degree of variability within samples and across all teeth provides a more nuanced understanding of dental wear among hunter-gatherers.

Surviving multi-trauma in the past.

BACKGROUND: Surviving multi-trauma is strongly associated with emergent resuscitation and treatment in modern medical facilities. Multi-trauma survival before the advent of modern medicine is likely to have been extremely uncommon, particularly in primitive societies. The aim of this study was to investigate the case of an ancient Australian Aboriginal who appeared to have survived multi-trauma. METHODS: We investigated the skeletal remains of an adult Australian Aboriginal with healed fractures of the right femur and humerus. The time of death was assessed by carbon dating. The sex and approximate age of the subject were assessed from the skeleton's morphometry. The fractured bones were assessed by visual inspection, plain radiographs and computer tomography (CT) scanning. RESULTS: The remains were of a male aged approximately 50 years at the time of his death, approximately 1000 years ago, preceding European settlement. Analysis of the malunions indicated that all fractures occurred in one traumatic event, normally a life-threatening injury combination, and that the subject survived for years after this incident, despite no or failed active treatment of his fractures. CONCLUSIONS: The survival of a man living in a primitive society after multi-trauma reflects an impressive depth of nursing and social support in a community of hunter gatherers.

Towards the identification of plant and animal binders on Australian stone knives.

There is limited information regarding the nature of plant and animal residues used as adhesives, fixatives and pigments found on Australian Aboriginal artefacts. This paper reports the use of FTIR in combination with the chemometric tools principal component analysis (PCA) and hierarchical clustering (HC) for the analysis and identification of Australian plant and animal fixatives on Australian stone artefacts. Ten different plant and animal residues were able to be discriminated from each other at a species level by combining FTIR spectroscopy with the chemometric data analysis methods, principal component analysis (PCA) and hierarchical clustering (HC). Application of this method to residues from three broken stone knives from the collections of the South Australian Museum indicated that two of the handles of knives were likely to have contained beeswax as the fixative whilst Spinifex resin was the probable binder on the third.