ABSTRACT
OBJECTIVE: To find out the optimum extract process for Ligusticum chuanxiong in Gan-ning Granule, and studyed the methods of concentration and dry for the extract. METHOD: With the yield of ferulic acid as the assessment index, to optimize the 80% alcohol totalling, extracting times and circumfluence time for extract process by the orthogonal design, to optimize the inlet-air temperature, feed speed and density of feed for spry drying by the orthogonal design. RESULT: The optimum procedure was the ferulic acid were extracted for 1 hour with 3 times of 80% alcohol. While extracting times effected it most porminently. The optimal processing conditions of spry drying were inlet-air temperature 120 degrees C, feed speed 8.5 mL x min(-1) and density of feed 1.15, While feed speed effected it most porminently. CONCLUSION: The experimental results provide the basis for the extraction process and drying process of the ferulic acid in ligusticum chuanxiong.
Subject(s)
Coumaric Acids/isolation & purification , Drugs, Chinese Herbal/isolation & purification , Ligusticum/chemistry , Plants, Medicinal/chemistry , Coumaric Acids/analysis , Coumaric Acids/chemistry , Desiccation/methods , Drugs, Chinese Herbal/analysis , Drugs, Chinese Herbal/chemistry , Technology, Pharmaceutical/methodsABSTRACT
OBJECTIVE: To establish a pre-column derivatization reversed-phase high-performance liquid chromatographic method for the determination of amino acids, which was known as the characteristic constituents of Fu Fang Banlangen Granulae. METHODS: We determined the amino acids in alkalescence by a pre-column derivatization of DNFB. NaAc buffer (pH 6.4)-Acetonitrile (850:150) was used as the mobile phase on a SinoChrom ODS-BP column. The detector was operated at 360 nm. RESULTS: The method was carried out over the range of 0.627 - 5. 016 microg for Arg and 0.874 - 7.000 microg for Pro. The recoveries were 97.7% with the RSD = 2.0% for Arg and 97.7% with the RSD = 1.5% for Pro. CONCLUSIONS: The method has good accuracy and repeatability and it can be used for the quality control of Fu Fang Banlangen Granulae.
Subject(s)
Amino Acids/analysis , Chromatography, High Pressure Liquid/methods , Drugs, Chinese Herbal/chemistry , Plants, Medicinal/chemistry , Arginine/analysis , Drug Combinations , Drugs, Chinese Herbal/isolation & purification , Proline/analysis , Quality Control , Reproducibility of Results , Sensitivity and Specificity , Time FactorsABSTRACT
A reversed-phase high performance liquid chromatographic method with pre-column derivatization for the determination of amino acids, which were known as the characteristic constituents of Isatis indigotica Fort was established. The amino acids in alkalescence were derivatized with 2, 4-dinitro-fluorobenzene (DNFB). A reproducible method for simultaneous qualitative analysis of glutamic acid (Glu), argirine (Arg) and proline (Pro) and quantitative analysis of Arg and Pro in Isatis indigotica Fort has been established. NaAc buffer (pH 6.4) acetonitrile (85:15, v/v) as mobile phase and a Sinochrom ODS-BP column were used. The detector was operated at 360 nm. The linear regressions of the standard curves were determined for Arg and for Pro. The method was carried out over the range of 0. 627 - 5.016 microg for Arg and 0. 874 - 7.000 microg for Pro. The recoveries were 98.5% and 98.4% with the relative standard deviations of 2.5% and 2.3% for Arg and Pro respectively. The results indicate that among the three amino acids in Isatis indigotica Fort, Arg content was the highest, Pro the second and Glu the lowest. The method has good accuracy and repeatability and it can be used for the quality control of Isatis indigotica Fort.
