[Pediatric supraglottic laryngeal hamartoma: a case report and literature review].

Laryngeal hamartoma is a benign proliferative tumor-like lesion that occurs in the larynx. A case of supraglotic laryngeal hamartoma admitted by our department and 12 cases of laryngeal hamartoma reported in literature were retrospectively analyzed, the pathogenesis, clinicalmanifestation, diagnosis, treatment and prognosis of laryngeal hamartoma was explored, aiming to improve the understanding and diagnosis and treatment.of this disease.

Expression of LATS1 in head and neck squamous cell carcinoma and its effect on tumor cell proliferation and invasion.

Nowadays, the incidence and mortality of head and neck tumors are gradually increasing. Head and neck malignant tumors (such as laryngeal cancer, hypopharyngeal cancer, oral cancer, nasopharyngeal cancer, oropharyngeal cancer, and other head and neck malignancies) are more common among systemic tumors. The most common pathological head and neck tumor type is squamous cell carcinoma, accounting for about 90%. In this study, immunohistochemical methods were used to collect the normal squamous epithelial tissues of the head and neck, atypical hyperplasia tissues, and head and neck squamous cell carcinomas on a tissue chip for detection. The recombinant LATS1 overexpression plasmid was prepared and transferred into B88 cells. Western blotting, MTT, and Transwell chamber methods were used to detect the effects of LATS1 proliferation, migration, and B88 cell overexpression. The experimental results showed that in head and neck squamous cell carcinoma, the expression of LATS1 protein decreased from 59.3% to 11.3%. At the same time, this protein inhibited the proliferation, migration, and invasion of head and neck squamous epithelial cells and also inhibited epithelium- Interstitial transformation exerts its tumor suppressor effect, indicating that LATS1 may play a tumor suppressor effect as a tumor suppressor gene. An in-depth study of the role and mechanism of LATS1 protein in the occurrence of head and neck squamous cell carcinoma may provide new opportunities for the treatment of head and neck squamous cell carcinoma in the future.

SIRT4 expression in laryngeal squamous cell carcinoma.

SIRT4 has been reported to be abnormally expressed in many malignant tumor tissues, but data in laryngeal squamous cell carcinoma (LSCC) is lacking. In the present study, we detected the expression of SIRT4 in 168 pairs of LSCC tissues and adjacent normal tissues using RT-qPCR, immunoblotting and immunohistochemical staining, and analyzed its clinical implication. We found that SIRT4 expression was low in LSCC tissues, and was significantly related to histological grade, T classification, clinical stage, lymph node metastasis and recurrence of LSCC patients. In vitro, knockdown of SIRT4 promoted the proliferation and migration of LSCC cells, while overexpression of SIRT4 inhibits the proliferation and migration of LSCC cells. Moreover, the expression of SIRT4 protein was an independent factor affecting the disease-free survival (DFS) (HR=0.562, 95%CI=0.1290.834) and overall survival rates (OS) (HR=0.628, 95%CI=0.267-0.935) of LSCC patients. The 5-years DFS and OS in LSCC patients with low SIRT4 expression were significantly lower than that in LSCC patients with high SIRT4 expression. In conclusion, SIRT4 was lowly expressed in LSCC patients, which might be related to more aggressive tumor behaviour and a poor prognosis.

GSTM1 null genotype may be associated with an increased nasopharyngeal cancer risk in South China: an updated meta-analysis and review.

Although many epidemiologic studies investigated the GSTM1 gene polymorphism and its association with nasopharyngeal carcinoma (NPC) in Chinese, definite conclusions cannot be drawn. To assess the impact of of GSTM1 polymorphism on the risk of NPC, an updated meta-analysis was performed in a Chinese population. A total of nine studies including 1,291 cases and 2,135 controls were involved in this meta-analysis. Meta-analysis of those nine studies showed that GSTM1 null genotype was associated with an increased risk of NPC in South China (odds ratio [OR] =1.47, 95% confidence interval [CI]: 1.27-1.70). In subgroup analyses stratified by source of controls, it revealed significant results in population-based studies (OR =1.40, 95% CI: 1.19-1.64). Additionally, a significant association was found in smokers (OR =3.16, 95% CI: 1.76-5.67). This meta-analysis indicated a marked association of GSTM1 with NPC risk in South China, and there might be an interaction between the polymorphism and smoking on NPC. However, further studies with gene-gene and gene-environment interactions are required for definite conclusions.