ABSTRACT
Polycystic ovary syndrome (PCOS) is a complex endocrine syndrome, resulting from the interaction of gene variants and environmental factors. PCOS is viewed as a proinflammatory state and is characterized by hyperandrogenism, hyperinsulinemia, and over-weight. In China, the incidence of PCOS is higher in the Uygur population than that in the Chinese Han population. The association of the tumor necrosis factor α (TNF-α) gene with PCOS remains to be clarified. Here, we investigated the association of TNF-α polymorphisms with PCOS in the Uygur population (393 patients with PCOS and 381 healthy subjects). Two single-nucleotide polymorphisms in TNF-α were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method: rs1800629 (-308G/A polymorphism), a commonly tested variant and rs4645843 (6213C/T polymorphism) that causes a Pro-to-Leu substitution at position 84, the most damaging variant of TNF-α based on in silico analysis. We thus found that both the genotypic and allelic distributions of rs4645843 were significantly different between PCOS and control groups (p = 0.03 and 0.024, respectively), whereas those of rs1800629 were similar between the groups. Furthermore, rs4645843 was significantly associated with serum testosterone levels and the score of Homeostatic Model Assessment for Insulin Resistance (HOMA-IR), but no such association was found with rs1800629. Importantly, both rs4645843 and rs1800629 were significantly associated with higher body mass index (p < 0.05). This is the first study that shows the association of TNF-α gene with PCOS in the Uygur population. The TNF-α gene may influence the pathogenesis of PCOS through regulating testosterone level, obesity and HOMA-IR.
Subject(s)
Ethnicity/genetics , Genetic Predisposition to Disease , Mutation, Missense/genetics , Polycystic Ovary Syndrome/genetics , Polymorphism, Single Nucleotide/genetics , Tumor Necrosis Factor-alpha/genetics , Adult , Case-Control Studies , Female , Haplotypes/genetics , Humans , Obesity/genetics , Risk FactorsABSTRACT
Omega-3 fatty acids, especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), have been shown to possess definitively suppressive effects on the growth of epithelial ovarian cancer cells. This study investigated the differential effects of pure EPA and DHA on the growth of epithelial ovarian cancer cells and the potential molecular mechanisms that may be involved. There were significant time- and dose-dependent inhibitory effects of both EPA and DHA on cellular proliferation of the epithelial ovarian cancer cell line TOV-21G (P < 0.05). TOV-21G cells pretreated with peroxisome proliferator receptor activator gamma (PPARγ) antagonist, GW9662, markedly suppressed EPA/DHA-induced apoptosis as determined by TUNEL assay, Annexin V-FITC/PI staining, and caspase-3 activity. EPA/DHA significantly induced PPARγ and p53 overexpression as observed in immunoblotting assay and the induction of p53 by EPA/DHA was abolished by GW9662. In all cases, the effect of DHA was significantly more potent than that of EPA (P < 0.05). Our findings suggested that DHA may be more effective than EPA in growth suppression of TOV-21G cells and the biologic effects may be partly mediated by PPARγ and p53 activation. Further research is required to elucidate additional divergent mechanisms to account for apparent differences between EPA and DHA.
Subject(s)
Docosahexaenoic Acids/pharmacology , Eicosapentaenoic Acid/pharmacology , Neoplasms, Glandular and Epithelial/drug therapy , Ovarian Neoplasms/drug therapy , Anilides/pharmacology , Apoptosis/drug effects , Carcinoma, Ovarian Epithelial , Caspase 3/metabolism , Cell Line, Tumor/drug effects , Cell Proliferation/drug effects , Docosahexaenoic Acids/administration & dosage , Dose-Response Relationship, Drug , Drug Screening Assays, Antitumor/methods , Eicosapentaenoic Acid/administration & dosage , Female , Humans , Neoplasms, Glandular and Epithelial/metabolism , Neoplasms, Glandular and Epithelial/pathology , Ovarian Neoplasms/metabolism , Ovarian Neoplasms/pathology , PPAR gamma/antagonists & inhibitors , PPAR gamma/metabolism , Tumor Suppressor Protein p53/metabolismABSTRACT
We reported the complete mitochondrial genome sequencing of an important cervical carcinoma model inbred rat strain for the first time. The total length of the mitogenome was 16,314 bp. It harbored 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and 1 non-coding control region. The mutation events contained in this strain were also reported.
Subject(s)
DNA, Mitochondrial/genetics , Endometrial Neoplasms/genetics , Genome, Mitochondrial/genetics , Mitochondria/genetics , Rats, Inbred Strains/genetics , Uterine Cervical Neoplasms/genetics , Animals , Base Composition/genetics , Base Sequence , Codon, Initiator/genetics , Codon, Terminator/genetics , Disease Models, Animal , Female , Genome Size/genetics , Molecular Sequence Data , Polymerase Chain Reaction , RNA, Ribosomal/genetics , RNA, Transfer/genetics , Rats , Sequence Analysis, DNAABSTRACT
OBJECTIVE: To evaluate the prevalence and associated risk factors of stress urinary incontinence(SUI) in adult women in Xinjiang. METHODS: In the cross-sectional study, 3403 Uygur women aged over 20 years were interviewed through a questionnaire of International Consultation on Incontinence Questionnaire Female Lower Urinary Tract Symptoms Module (ICIQ-FLUTS) and the adult questionnaire were used in our research. All parts of the content were according to the characteristics of women in Xinjiang and the purpose of our research. The risk factors were studied by Logistic regression analysis. RESULTS: The prevalence of urinary incontinence(UI) was 41.96% (1428/3403) and 28.21% (960/3403) of SUI in Uygur women. In multivariate logistic regression analysis, the risk factors of SUI are body mass index (OR = 1.672, 95%CI:1.082-2.584), parity (OR = 5.092, 95%CI:3.889-6.666), neonatal birth weight (OR = 5.623, 95%CI:3.335-9.480), the mode of delivery (OR = 2.247, 95%CI:1.634-3.090), the lateral episiotomy (OR = 4.448, 95%CI:3.112-6.357), menopause(OR = 5.145, 95%CI: 3.613-7.328), chronic pelvic pain (OR = 3.869, 95%CI:1.051-14.250), pelvic organ prolapse (OR = 3.501, 95%CI:2.508-4.887). CONCLUSION: The incidence of SUI is related with multiple factors, especially with obesity and the obstetric factor.
