Practical method for improving the accuracy of weakly stressed birefringence detection by circularly polarized instantaneous photoelasticity.

Due to the mismatch between the quarter-wave plate and the wavelength of the light source, the circularly polarized field photoelastic stress analysis method has an impact on the measurement of the phase delay and the stress direction angle. In particular, the measurement of the phase delay is inaccurate for weakly stressed samples with phase delays less than a quarter-wavelength. In this paper, we first give a method for calculating the mismatch value δ, which requires only one air calibration without prior calibration of the parameters of the quarter-wave plate with other equipment. We then introduce δ into the correction process for the phase delay, derive the correction equation, and give a theoretical comparison of the relative error curves. The results show that the correction method can theoretically limit the maximum amount of error. Finally, we have verified the accuracy of the method by measurements of the internal lens stress before and after the correction and by stitching measurements on SiC wafers.

Application of mixed reality-based surgical navigation system in craniomaxillofacial trauma bone reconstruction.

OBJECTIVES: This study aimed to build a surgical navigation system based on mixed reality (MR) and optical positioning technique and evaluate its clinical applicability in craniomaxillofacial trauma bone reconstruction. Me-thods We first integrated the software and hardware platforms of the MR-based surgical navigation system and explored the system workflow. The systematic error, target registration error, and osteotomy application error of the system were then analyzed via 3D printed skull model experiment. The feasibility of the MR-based surgical navigation system in craniomaxillofacial trauma bone reconstruction was verified via zygomatico-maxillary complex (ZMC) reduction experiment of the skull model and preliminary clinical study. RESULTS: The system error of this MR-based surgical navigation system was 1.23 mm±0.52 mm, the target registration error was 2.83 mm±1.18 mm, and the osteotomy application error was 3.13 mm±1.66 mm. Virtual surgical planning and the reduction of the ZMC model were successfully conducted. In addition, with the guidance of the MR-based navigation system, the frontal bone defect was successfully reconstructed, and the clinical outcome was satisfactory. CONCLUSIONS: The MR-based surgical navigation system has its advantages in virtual reality fusion effect and dynamic navigation stability. It provides a new method for doctor-patient communications, education, preoperative planning, and intraoperative navigation in craniomaxillofacial surgery.

A novel motionless calibration method for augmented reality surgery navigation system based on optical tracker.

Augmented reality (AR) surgery navigation systems display the pre-operation planned virtual model at the accurate position in the real surgical scene to assist the operation. Accurate calibration of the mapping relationship between the virtual coordinate and the real world is the key to the virtual-real fusion effect. Former calibration methods require the doctor user to conduct complex manual procedures before usage. This paper introduces a novel motionless virtual-real calibration method. The method only requires to take a mixed reality image containing both virtual and real marker balls using the built-in forward camera of the AR glasses. The mapping relationship between the virtual and real spaces is calculated by using the camera coordinate system as a transformation medium. The composition and working process of the AR navigation system is introduced, and then the mathematical principle of the calibration is designed. The feasibility of the proposed calibration scheme is verified with a verification experiment, and the average registration accuracy of the scheme is around 5.80mm, which is of same level of formerly reported methods. The proposed method is convenient and rapid to implement, and the calibration accuracy is not dependent on the user experience. Further, it can potentially realize the real-time update of the registration transformation matrix, which can improve the AR fusion accuracy when the AR glasses moves. This motionless calibration method has great potential to be applied in future clinical navigation research.

Biomimetic apposition compound eye fabricated using microfluidic-assisted 3D printing.

After half a billion years of evolution, arthropods have developed sophisticated compound eyes with extraordinary visual capabilities that have inspired the development of artificial compound eyes. However, the limited 2D nature of most traditional fabrication techniques makes it challenging to directly replicate these natural systems. Here, we present a biomimetic apposition compound eye fabricated using a microfluidic-assisted 3D-printing technique. Each microlens is connected to the bottom planar surface of the eye via intracorporal, zero-crosstalk refractive-index-matched waveguides to mimic the rhabdoms of a natural eye. Full-colour wide-angle panoramic views and position tracking of a point source are realized by placing the fabricated eye directly on top of a commercial imaging sensor. As a biomimetic analogue to naturally occurring compound eyes, the eye's full-colour 3D to 2D mapping capability has the potential to enable a wide variety of applications from improving endoscopic imaging to enhancing machine vision for facilitating human-robot interactions.

A rapid nucleic acid concentration measurement system with large field of view for a droplet digital PCR microfluidic chip.

Droplet digital polymerase chain reaction (ddPCR) is an effective technique, with unparalleled sensitivity, for the absolute quantification of target nucleic acids. However, current commercial ddPCR devices for detecting the gene chip are time consuming due to complex image stitching. To address this issue, we propose a universal concentration determination system and realize one-time gene chip imaging with high resolution. All the functional units are controlled by self-developed software using the PyQt5 module in Python. Without stitching technology, images of the ddPCR chip (28 mm × 18 mm) containing 20 000 independent 0.81 nL micro chambers can be obtained in less than 15 seconds, which saves about 165 seconds. A white laser light source (2 mW cm-2) was employed as a substitute for the mercury lamp. Its wavelength matches well with typical fluorescent dyes (e.g., HEX, ROX and Cy5), and thus it can strengthen the fluorescence intensity for weak signals. The results also demonstrated that the correlation coefficient for the measured concentration and theoretical value was above 99%, by testing the ddPCR products with COVID-19 virus. Such a system can greatly reduce the time required for image acquisition and DNA concentration determination, and thus is able to speed up the lab-to-application process for ddPCR technology.

Electrodeposition immobilized molybdenum disulfide quantum dots and their electrochemiluminescence application in the detection of melamine residues in milk powder.

In this paper, one-step hydrothermal and electrodeposition methods were used to prepare a MoS2 quantum dot (QD) solid-phase electrochemiluminescent (ECL) electrode for the detection of melamine residues in milk powder. With the assistance of chitosan, MoS2 QDs fixed by the one-step electrodeposition method show better ECL performance than those by traditional deposition methods due to better dispersibility and stability. Based on the quenching of the MoS2 QDs ECL signal by melamine, quantitative detection of melamine in the sample was performed. The structure and morphology of a MoS2-CHIT/indium tin oxide (ITO) solid-phase ECL electrode were characterized by TEM and XPS, and melamine was detected by the ECL method using a three-electrode system. The proposed sensor exhibited good linearity in the range of 1.00 × 10-11 to 1.00 × 10-7 mol L-1 (ΔI = 12 100.62 + 1009.93 lg c (mol L-1), R2 = 0.997), and the method shows the advantages of simplicity and sensitivity compared to traditional detection methods. The interference of common ions in milk powder on the modified electrode was within 5%, and the recovery rate of real sample detection was within 97-98%. As a result, the proposed method is suitable for detecting melamine residues in milk powder.

Molecular Engineering of Aromatic Imides for Organic Secondary Batteries.

Aromatic imides are a class of attractive organic materials with inherently electroactive groups and large π electron-deficient scaffolds, which hold potential as electrode materials for organic secondary batteries (OSBs). However, the undecorated aromatic imides are usually plagued by low capacity, high solubility in electrolyte, and poor electronic/ionic conductivity. Molecular engineering has been demonstrated to be an effective strategy to address unsatisfying characteristics of the aromatic imides, thereby expanding their scope for applications in OSBs. In this review, the recent research progress in modulation of the capacity, dissolution, and electronic/ionic conductivity of aromatic imides for organic lithium batteries, organic sodium batteries, and redox flow batteries are summarized. In addition, the challenge and prospective of aromatic imides in organic secondary battery applications are also discussed.

Polyvinylpyrrolidone-assisted synthesis of highly water-stable cadmium-based metal-organic framework nanosheets for the detection of metronidazole.

Recently, much effort has been dedicated to ultra-thin two-dimensional metal-organic framework (2D MOF) nanosheets due to their outstanding properties, such as ultra-thin morphology, large specific surface area, abundant modifiable active sites, etc. However, the preparation of high-quality 2D MOF nanosheets in good yields still remains a huge challenge. Herein, we report 2D cadmium-based metal-organic framework (Cd-MOF) nanosheets prepared in a one-pot polyvinylpyrrolidone (PVP)-assisted synthesis method with high yield. The Cd-MOF nanosheets were characterized with good stability and dispersion in aqueous systems, and were highly selective and sensitive to the antibiotic metronidazole (MNZ) with low limit of detection (LOD: 0.10 µM), thus providing a new and promising fluorescent sensor for rapid detection of MNZ in aqueous solution.

High-throughput and uniform large field-of-view multichannel fluorescence microscopy with super-thin dichroism for a dPCR gene chip.

With the rapid development of digital precision medicine, the digital polymerase chain reaction (dPCR) deoxyribonucleic acid (DNA) gene chip integrates more channels with smaller size and larger area, which leads to a higher technical requirement for commercial optical fluorescence microscopy. The multitime image splicing method is widely used for DNA detection. However, it consumes time and has visible seamless image results. This work has demonstrated the design and fabrication of a three channel reversed and reduced fluorescence microscopic imaging system with high-resolution and large field of view for one-time imaging. We introduced the super ultra-thin dichroic mirror into the space between the objective lens and the gene chip to achieve a uniform illumination and a strong signal for the large area gene chip. The fabricated new fluorescence microscopy can take a one-time imaging for the 28×18mm dPCR gene chip with more than 20,000 multi micro-droplets within FAM, HEX, and ROX fluorescence channels. The optical system was designed with a numerical aperture (NA) of 0.106. Modulation transfer function (MTF) is higher than 0.675 at 70 lp/mm, and the function resolution capability is 10 µm with the whole magnification of -0.65times. The fly's eye lens-based illumination system was tested with a uniform output of over 90% in the whole ϕ34.7mm chip area. The design was tested, and the experimental results showed that this new system provides a fast, efficient, and professional optical imaging method for detection of the new emerged digital PCR gene chip, which has larger area and more channels.

Experimental analysis of a wavefront coding system with a phase plate in different surfaces.

The application of an integrated phase plate can greatly simplify an optical system structure due to the defocus insensitivity and aberration inhibition of the wavefront coding imaging technology. In addition, the development of free-form surface technology promotes the application of integrated phase plates. In order to view the imaging characteristics of the integrated wavefront coding system, we built two kinds of wavefront coding systems with the phase plate integrated in different surfaces of the optical system. Simulation results show that phase plates with the same parameters but in different surfaces can realize consistent results. For further observation, two separated phase plates with the same parameters are processed, and, correspondingly, experiments of microscopic objective imaging are carried out. Experimental results confirm that the phase plate can be successfully applied to increase the design flexibility of wavefront coding, leading to a potential mass application solution.

Colour compound lenses for a portable fluorescence microscope.

In this article, we demonstrated a handheld smartphone fluorescence microscope (HSFM) that integrates dual-functional polymer lenses with a smartphone. The HSFM consists of a smartphone, a field-portable illumination source, and a dual-functional polymer lens that performs both optical imaging and filtering. Therefore, compared with the existing smartphone fluorescence microscope, the HSFM does not need any additional optical filters. Although fluorescence imaging has traditionally played an indispensable role in biomedical and clinical applications due to its high specificity and sensitivity for detecting cells, proteins, DNAs/RNAs, etc., the bulky elements of conventional fluorescence microscopes make them inconvenient for use in point-of-care diagnosis. The HSFM demonstrated in this article solves this problem by providing a multifunctional, miniature, small-form-factor fluorescence module. This multifunctional fluorescence module can be seamlessly attached to any smartphone camera for both bright-field and fluorescence imaging at cellular-scale resolutions without the use of additional bulky lenses/filters; in fact, the HSFM achieves magnification and light filtration using a single lens. Cell and tissue observation, cell counting, plasmid transfection evaluation, and superoxide production analysis were performed using this device. Notably, this lens system has the unique capability of functioning with numerous smartphones, irrespective of the smartphone model and the camera technology housed within each device. As such, this HSFM has the potential to pave the way for real-time point-of-care diagnosis and opens up countless possibilities for personalized medicine.

Design and Fabrication of Wafer-Level Microlens Array with Moth-Eye Antireflective Nanostructures.

Wafer-level packaging (WLP) based camera module production has attracted widespread industrial interest because it offers high production efficiency and compact modules. However, suppressing the surface Fresnel reflection losses is challenging for wafer-level microlens arrays. Traditional dielectric antireflection (AR) coatings can cause wafer warpage and coating fractures during wafer lens coating and reflow. In this paper, we present the fabrication of a multiscale functional structure-based wafer-level lens array incorporating moth-eye nanostructures for AR effects, hundred-micrometer-level aspherical lenses for camera imaging, and a wafer-level substrate for wafer assembly. The proposed fabrication process includes manufacturing a wafer lens array metal mold using ultraprecise machining, chemically generating a nanopore array layer, and replicating the multiscale wafer lens array using ultraviolet nanoimprint lithography. A 50-mm-diameter wafer lens array is fabricated containing 437 accurate aspherical microlenses with diameters of 1.0 mm; each lens surface possesses nanostructures with an average period of ~120 nm. The microlens quality is sufficient for imaging in terms of profile accuracy and roughness. Compared to lenses without AR nanostructures, the transmittance of the fabricated multiscale lens is increased by ~3% under wavelengths of 400-750 nm. This research provides a foundation for the high-throughput and low-cost industrial application of wafer-level arrays with AR nanostructures.

(E)-2-Phenyl-N-tosyl-non-2-en-4-ynamide.

The mol-ecule of the title compound, C22H23NO3S, adopts an E conformation about the C=C bond. The dihedral angle between the benzene rings is 23.79 (5)°. In the crystal, pairs of N-H⋯O hydrogen bonds link the mol-ecules, forming inversion dimers. The terminal butyl group is disordered over two sets of sites in a 0.559 (6):0.441 (6) ratio.

Quasi-common-path laser feedback interferometry based on frequency shifting and multiplexing.

We present a quasi-common-path laser feedback interferometer based on frequency shifting and multiplexing. The interferometer uses two acousto-optic modulators to shift the frequency of the target-generated feedback light by 2 Omega. A properly aligned mirror is inserted into the feedback path to generate a feedback light frequency shifted by Omega. Phase variations of the two quasi-common-path feedback light beams are simultaneously measured through heterodyne demodulation with two different reference signals. Their subtraction accurately reflects the target displacement. Under typical room conditions, the system's short-period resolution is better than 2 nm, and its 3 min displacement accuracy is 8 nm.

[Determination of deoxyribonucleic acid with acridine orange by resonance light scattering technique].

The resonance light scattering (RLS) spectra of acridine orange with deoxyribonucleic acid have been studied. The RLS of acridine orange is greatly enhanced by deoxyribonucleic acid in pH 11. There is a resonance light scattering peak at 324 nm. Under optimal conditions, the linear ranges of the calibration curves are 7.5 x 10(-8) -9.8 x 10(-6) g x mL(-1) for Calf thymus DNA (ct DNA), 3.1 x 10(-8) -7.3 x 10(-6) g x mL(-1) for Fish sperm DNA (fs DNA) with detection limits 20.8 ng x mL(-1) for ct DNA and 20.5 ng x mL(-1) for fs DNA. This method is simple, selectivity and sensitivity has been applied to the determination of deoxyribonucleic acid in mixed samples with satisfactory results.