Establishing preanalytical stability of vitamin A and vitamin E.

Vitamin A and Vitamin E, a group of lipid-soluble vitamins, can be degraded by photolysis and photooxidation after exposure to light. As an essential area of the preanalytical stage, inappropriate storage conditions of patient samples could lead to inaccurate results. In this study, we evaluated three of the most common preanalytical storage conditions (RT, +4°C, and -20 °C) in the workflow in the clinical laboratory setting using both clear and amber Eppendorf tubes. Analyte stability in each storage condition was tested with five patient pools. After 24 hrs at RT, a significant decrease in vitamin A and E was observed for samples stored in clear Eppendorf tubes (19.2 % and 17.9 %, for vitamin A and E, respectively), compared with samples stored in amber Eppendorf tubes (9.1 % and 7.3 %), respectively. After the specimen was stored at 4 °C for 7 days, vitamin A concentrations decreased in clear tubes by 14.7 % and in amber tubes by 12.7 %. Similarly, vitamin E concentrations decreased in both clear and amber tubes by 11.2 % and 13.4 %, respectively. Both vitamin A and vitamin E demonstrated acceptable stability in clear and amber Eppendorf tubes at -20 °C for up to 22 days. It is preferable to use amber tubes to protect vitamin A and E from light during short term storage. Storage of vitamin A and E specimens beyond 7 days should be at -20 °C.

Quantitative filter forensics for semivolatile organic compounds in social housing apartments.

Residents from low-income social housing are vulnerable to adverse health effects from indoor air pollution. Particle-bound concentrations of eight phthalates and 12 polycyclic aromatic hydrocarbons (PAHs) in indoor air were measured using quantitative filter forensics with portable air cleaners deployed for three one-week periods from 2015 to 2017. The sample included 143 apartments across seven multi-unit social housing buildings in Toronto, Canada, that went through energy retrofits in 2016. Eight phthalates and six PAHs were found in more than 50% of the apartments in either of the three sampling periods. Di(2-ethylhexyl) phthalate (DEHP) and phenanthrene were the dominant phthalate and PAH, with median concentrations of 146, 143, and 130 ng/m3 and 1.51, 0.58, and 0.76 ng/m3 in the late spring of 2015, and after retrofits in late spring 2017 and winter of 2017, respectively. SVOC concentrations were generally lower after energy retrofits, with significant differences for phenanthrene, fluoranthene, and pyrene. Lower concentrations post-retrofit may be related to less overheating and less need for opening windows. Concentrations of phthalates and PAHs in this study were similar to or higher than those reported in the literature. Results suggest that the use of portable air filters is a promising method to assess concentrations of indoor particle-bound SVOCs.

Indoor exposure to phthalates and polycyclic aromatic hydrocarbons (PAHs) to Canadian children: the Kingston allergy birth cohort.

BACKGROUND: Canadian children are widely exposed to phthalates and polycyclic aromatic hydrocarbons (PAHs) from indoor sources. Both sets of compounds have been implicated in allergic symptoms in children. OBJECTIVE: We characterize concentrations of eight phthalates and 12 PAHs in floor dust from the bedrooms of 79 children enrolled in the Kingston Allergy Birth Cohort (KABC). METHOD: Floor dust was collected from the bedrooms of 79 children who underwent skin prick testing for common allergens after their first birthday. Data were collected on activities, household, and building characteristics via questionnaire. RESULTS: Diisononyl phthalate (DiNP) and phenanthrene were the dominant phthalate and PAH with median concentrations of 561 µg/g and 341 ng/g, respectively. Benzyl butyl phthalate (BzBP) and chrysene had the highest variations among all tested homes, ranging from 1-95% to 1-99%, respectively. SIGNIFICANCE: Some phthalates were significantly associated with product and material use such as diethyl phthalate (DEP) with fragranced products and DiNP and DiDP with vinyl materials. Some PAHs were significantly associated with household characteristics, such as benzo[a]pyrene with smoking, and phenanthrene and fluoranthene with the presence of an attached garage. Socioeconomic status (SES) had positive and negative relationships with some concentrations and some explanatory factors. No significant increases in risk of atopy (positive skin prick test) was found as a function of phthalate or PAH dust concentrations.

Novel Bayesian Method to Derive Final Adjusted Values of Physicochemical Properties: Application to 74 Compounds.

Accurate values of physicochemical properties are essential for screening semivolatile organic compounds for human and environmental hazard and risk. In silico approaches for estimation are widely used, but the accuracy of these and measured values can be difficult to ascertain. Final adjusted values (FAVs) harmonize literature-reported measurements to ensure consistency and minimize uncertainty. We propose a workflow, including a novel Bayesian approach, for estimating FAVs that combines measurements using direct and indirect methods and in silico values. The workflow was applied to 74 compounds across nine classes to generate recommended FAVs (FAVRs). Estimates generated by in silico methods (OPERA, COSMOtherm, EPI Suite, SPARC, and polyparameter linear free energy relationships (pp-LFER) models) differed by orders of magnitude for some properties and compounds and performed systematically worse for larger, more polar compounds. COSMOtherm and OPERA generally performed well with low bias although no single in silico method performed best across all compound classes and properties. Indirect measurement methods produced highly accurate and precise estimates compared with direct measurement methods. Our Bayesian method harmonized measured and in silico estimated physicochemical properties without introducing observable biases. We thus recommend use of the FAVRs presented here and that the proposed Bayesian workflow be used to generate FAVRs for SVOCs beyond those in this study.

Challenges in the Analysis of Novel Flame Retardants in Indoor Dust: Results of the INTERFLAB 2 Interlaboratory Evaluation.

The Interlaboratory Study of Novel Flame Retardants (INTERFLAB 2) was conducted by 20 laboratories in 12 countries to test the precision and accuracy of the analysis of 24 "novel" flame retardants (NFRs). Laboratories analyzed NFRs in injection-ready test mixtures, in extracts of residential dust, and in residential dust to evaluate the influence of dust handling and extraction. For test mixtures, mean reported concentrations of PBT, PBEB, EH-TBB, TBBPA, TBDP-TAZTO, TBOEP, α-TBCO, ß-DBE-DBCH, and total HBCDD differed by >25% relative to reference values. Coefficients of variation were higher in dusts/dust extracts than in test mixtures. Concentrations among laboratories ranged over 3-4 orders of magnitude for HBB, TBP-DBPE, TBP-AE, and TDCIPP in dust extracts and dusts. Most laboratories produced repeatable dust concentrations, but differences reported in the literature among laboratories of <70% could be due to analytical variability, and the attribution of such differences to other causes should be made with caution. Most variations in accuracy and precision were introduced by matrix effects and/or sample processing, rather than instrumental analysis. We recommend recovery correction to improve accuracy. There is a need to improve analytical methods and to validate methods on complex matrices such as standard reference materials for dust or spiked matrices.