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1.
Am J Transplant ; 15(1): 64-75, 2015 Jan.
Article in English | MEDLINE | ID: mdl-25403742

ABSTRACT

The ability to generate patient-specific cells through induced pluripotent stem cell (iPSC) technology has encouraged development of three-dimensional extracellular matrix (ECM) scaffolds as bioactive substrates for cell differentiation with the long-range goal of bioengineering organs for transplantation. Perfusion decellularization uses the vasculature to remove resident cells, leaving an intact ECM template wherein new cells grow; however, a rigorous evaluative framework assessing ECM structural and biochemical quality is lacking. To address this, we developed histologic scoring systems to quantify fundamental characteristics of decellularized rodent kidneys: ECM structure (tubules, vessels, glomeruli) and cell removal. We also assessed growth factor retention--indicating matrix biofunctionality. These scoring systems evaluated three strategies developed to decellularize kidneys (1% Triton X-100, 1% Triton X-100/0.1% sodium dodecyl sulfate (SDS) and 0.02% Trypsin-0.05% EGTA/1% Triton X-100). Triton and Triton/SDS preserved renal microarchitecture and retained matrix-bound basic fibroblast growth factor and vascular endothelial growth factor. Trypsin caused structural deterioration and growth factor loss. Triton/SDS-decellularized scaffolds maintained 3 h of leak-free blood flow in a rodent transplantation model and supported repopulation with human iPSC-derived endothelial cells and tubular epithelial cells ex vivo. Taken together, we identify an optimal Triton/SDS-based decellularization strategy that produces a biomatrix that may ultimately serve as a rodent model for kidney bioengineering.


Subject(s)
Endothelium, Vascular/cytology , Extracellular Matrix/physiology , Induced Pluripotent Stem Cells/cytology , Kidney Tubules/physiology , Organ Transplantation/standards , Tissue Engineering , Tissue Scaffolds , Animals , Cell Differentiation , Cells, Cultured , Detergents/pharmacology , Humans , Kidney Tubules/blood supply , Kidney Tubules/drug effects , Male , Perfusion , Rats , Rats, Sprague-Dawley
2.
Clin Genet ; 80(4): 305-18, 2011 Oct.
Article in English | MEDLINE | ID: mdl-21651512

ABSTRACT

Rab GTPases are well-recognized targets in human disease, although are underexplored therapeutically. Elucidation of how mutant or dysregulated Rab GTPases and accessory proteins contribute to organ specific and systemic disease remains an area of intensive study and an essential foundation for effective drug targeting. Mutation of Rab GTPases or associated regulatory proteins causes numerous human genetic diseases. Cancer, neurodegeneration and diabetes represent examples of acquired human diseases resulting from the up- or downregulation or aberrant function of Rab GTPases. The broad range of physiologic processes and organ systems affected by altered Rab GTPase activity is based on pivotal roles in responding to cell signaling and metabolic demand through the coordinated regulation of membrane trafficking. The Rab-regulated processes of cargo sorting, cytoskeletal translocation of vesicles and appropriate fusion with the target membranes control cell metabolism, viability, growth and differentiation. In this review, we focus on Rab GTPase roles in endocytosis to illustrate normal function and the consequences of dysregulation resulting in human disease. Selected examples are designed to illustrate how defects in Rab GTPase cascades alter endocytic trafficking that underlie neurologic, lipid storage, and metabolic bone disorders as well as cancer. Perspectives on potential therapeutic modulation of GTPase activity through small molecule interventions are provided.


Subject(s)
Endocytosis/physiology , rab GTP-Binding Proteins/metabolism , Animals , Autophagy , Biological Transport , Cell Membrane/metabolism , Humans , Signal Transduction , rab GTP-Binding Proteins/genetics
5.
J Cell Biol ; 149(1): 111-24, 2000 Apr 03.
Article in English | MEDLINE | ID: mdl-10747091

ABSTRACT

Cystogenesis associated with autosomal dominant polycystic kidney disease (ADPKD) is characterized by perturbations in the polarized phenotype and function of cyst-lining epithelial cells. The polycystins, the protein products of the genes mutated in the majority of ADPKD cases, have been described recently, but the pathological mechanism by which causal mutations result in the mislocalization of cell membrane proteins has remained unclear. This report documents the dissociation from the ADPKD cell basolateral membrane of three molecules essential for spatial organization and exocytosis. The adherens junction protein E-cadherin, the subcellular disposition of which governs intercellular and intracellular architecture, was discovered sequestered in an internal ADPKD cell compartment. At the same time, sec6 and sec8, components of a complex critical for basolateral cargo delivery normally arrayed at the apico-lateral apex, were depleted from the ADPKD cell plasma membrane. An analysis of membrane transport revealed that basolateral trafficking of proteins and lipids was impaired as a result of delayed cargo exit from the ADPKD cell Golgi apparatus. Apical transport proceeded normally. Taken together with recent documentation of an association between polycystin-1 and E-cadherin (Huan and van Adelsberg 1999), the data suggest that causal mutations disrupt E-cadherin-dependent cytoarchitecture, adversely affecting protein assemblies crucial for basolateral trafficking.


Subject(s)
Cell Polarity , Cytoskeleton/pathology , Exocytosis , Genes, Dominant/genetics , Polycystic Kidney, Autosomal Dominant/metabolism , Polycystic Kidney, Autosomal Dominant/pathology , Biological Transport , Cadherins/metabolism , Carrier Proteins/metabolism , Cell Adhesion , Cell Membrane/chemistry , Cell Membrane/metabolism , Cells, Cultured , Cytoskeleton/metabolism , Epithelial Cells/pathology , Fluorescent Antibody Technique , Golgi Apparatus/metabolism , Humans , Kinetics , Membrane Proteins/genetics , Membrane Proteins/physiology , Polycystic Kidney, Autosomal Dominant/genetics , Protein Binding , Protein Processing, Post-Translational , Proteins/genetics , Proteins/physiology , TRPP Cation Channels , Tight Junctions/metabolism , Vesicular Transport Proteins
6.
J Cell Sci ; 113 Pt 2: 183-92, 2000 Jan.
Article in English | MEDLINE | ID: mdl-10633070

ABSTRACT

Endocytosis is characterized by vesicular transport along numerous pathways. Common steps in each pathway include membrane budding to form vesicles, transport to a particular destination, and ultimately docking and fusion with the target membrane. Specificity of vesicle targeting is rendered in part by associated Rab GTPases. This review summarizes current knowledge about Rab GTPase functions in the endocytic pathways and provides insight into the regulation of Rab GTPase activity and mechanisms of Rab protein function. Functional assays have identified some Rab proteins that operate on individual pathways, but Rab proteins in several pathways remain controversial or have not been identified. Control of Rab GTPase activity is exerted through multiple levels of regulation. Significant new information pertaining to Rab protein function in regulating transport has emerged. Remarkably, Rab5 GTPase links budding, cytoskeletal transport and docking/fusion activities. This paradigm will most likely be generally applicable to other Rab GTPase pathways. Together with the cross-talk between different Rab proteins and their effectors, this may provide an integrated system for the general coordination of endocytic pathways to maintain organelle homeostasis.


Subject(s)
Endocytosis/physiology , rab GTP-Binding Proteins/physiology , Animals , Biological Transport, Active , Cell Polarity , Cytoskeleton/physiology , Epithelial Cells/physiology , Humans , Membrane Fusion/physiology
7.
Traffic ; 1(8): 675-86, 2000 Aug.
Article in English | MEDLINE | ID: mdl-11208155

ABSTRACT

Epithelial cells explanted from autosomal dominant polycystic kidney disease (ADPKD) tissue exhibit impaired exocytosis, specifically between the Golgi and basolateral membrane (Charron A, Nakamura B, Bacallo R, Wandinger-Ness A. Compromised cytoarchitecture and polarized trafficking in autosomal dominant polycystic kidney disease cells. J Cell Biol 2000; 148: 111-124.). Here the defect is shown to result in the accumulation of the basolateral transport marker vesicular stomatitis virus (VSV) G protein in the Golgi complex. Golgi complex morphology is consequently altered in the disease cells, evident in the noticeable fenestration and dilation of the cisternae. Further detailed microscopic evaluation of normal kidney and ADPKD cells revealed that ineffective basolateral exocytosis correlated with modulations in the localization of select post-Golgi transport effectors. The cytosolic coat proteins p200/myosin II and caveolin exhibited enhanced association with the cytoskeleton or the Golgi of the disease cells, respectively. Most cytoskeletal components with known roles in vesicle translocation or formation were normally arrayed with the exception of Golgi beta-spectrin, which was less prevalent on vesicles. The rab8 GTPase, important for basolateral vesicle targeting, was redistributed from the perinuclear Golgi region to disperse vesicles in ADPKD cells. At the basolateral membrane of ADPKD cells, there was a notable loss of the exocyst components sec6/sec8 and an unidentified syntaxin. It is postulated that dysregulated basolateral transport effector function precipitates the disruption of basolateral exocytosis and dilation of the ADPKD cell Golgi as basolateral cargo accumulates within the cisternae.


Subject(s)
Epithelium/metabolism , Exocytosis/physiology , Golgi Apparatus/metabolism , Intracellular Membranes/metabolism , Kidney Tubules/metabolism , Membrane Glycoproteins , Polycystic Kidney, Autosomal Dominant/metabolism , Cells, Cultured/metabolism , Cells, Cultured/pathology , Cells, Cultured/ultrastructure , Epithelium/pathology , Epithelium/physiopathology , Golgi Apparatus/pathology , Golgi Apparatus/ultrastructure , Humans , Intracellular Membranes/pathology , Intracellular Membranes/ultrastructure , Kidney Tubules/pathology , Kidney Tubules/physiopathology , Polycystic Kidney, Autosomal Dominant/pathology , Polycystic Kidney, Autosomal Dominant/physiopathology , Protein Transport/physiology , Transport Vesicles/metabolism , Viral Envelope Proteins/metabolism
8.
Am J Physiol ; 277(5): H1985-96, 1999 11.
Article in English | MEDLINE | ID: mdl-10564155

ABSTRACT

The microvascular wall is remarkably simple, consisting only of the endothelial lining, subjacent basal lamina, and underlying periendothelial cells. This study describes the characterization of a novel microvascular protein. This 80,000-molecular weight protein was predominantly associated with electron-lucent amorphous material in capillary basal laminae and therefore termed cablin (protein of the capillary basal lamina). Consistent with its immunolocalization to the microvasculature, cablin was synthesized and secreted by cultured endothelial cells and vascular smooth muscle cells. Furthermore, cablin expression was induced during neovascularization. The predicted amino acid sequence of cablin revealed a prevalence of polar amino acids. Accounting for the low yet significant homology to several alpha-helical proteins, these residues were best accommodated by secondary structure predictions that aligned the molecule into two large alpha-helical domains. The presence of the integrin-binding RGD tripeptide and a putative elastin-binding sequence suggest that this rodlike molecule is suited to cross-link cells and matrix constituents. In this capacity it could contribute to the mechanical strength or the angiogenic potential of the microvasculature.


Subject(s)
Capillaries/metabolism , Proteins/metabolism , Amino Acid Sequence/genetics , Animals , Base Sequence/genetics , Basement Membrane/metabolism , Blood Vessels/cytology , Blood Vessels/metabolism , Cattle , DNA, Complementary/genetics , DNA, Complementary/isolation & purification , Humans , Male , Molecular Sequence Data , Molecular Weight , Proteins/chemistry , Proteins/genetics , Rats , Rats, Sprague-Dawley , Retinal Neovascularization/metabolism , Tissue Distribution
9.
Mol Biol Cell ; 9(11): 3241-57, 1998 Nov.
Article in English | MEDLINE | ID: mdl-9802909

ABSTRACT

The rab11 GTPase has been localized to both the Golgi and recycling endosomes; however, its Golgi-associated function has remained obscure. In this study, rab11 function in exocytic transport was analyzed by using two independent means to perturb its activity. First, expression of the dominant interfering rab11S25N mutant protein led to a significant inhibition of the cell surface transport of vesicular stomatitis virus (VSV) G protein and caused VSV G protein to accumulate in the Golgi. On the other hand, the expression of wild-type rab11 or the activating rab11Q70L mutant had no adverse effect on VSV G transport. Next, the membrane association of rab11, which is crucial for its function, was perturbed by modest increases in GDP dissociation inhibitor (GDI) levels. This led to selective inhibition of the trans-Golgi network to cell surface delivery, whereas endoplasmic reticulum-to-Golgi and intra-Golgi transport were largely unaffected. The transport inhibition was reversed specifically by coexpression of wild-type rab11 with GDI. Under the same conditions two other exocytic rab proteins, rab2 and rab8, remained membrane bound, and the transport steps regulated by these rab proteins were unaffected. Neither mutant rab11S25N nor GDI overexpression had any impact on the cell surface delivery of influenza hemagglutinin. These data show that functional rab11 is critical for the export of a basolateral marker but not an apical marker from the trans-Golgi network and pinpoint rab11 as a sensitive target for inhibition by excess GDI.


Subject(s)
Cell Membrane/metabolism , Exocytosis/physiology , GTP-Binding Proteins/metabolism , Golgi Apparatus/metabolism , Guanine Nucleotide Dissociation Inhibitors , Membrane Glycoproteins , rab GTP-Binding Proteins , Animals , Biological Transport , Cell Line , Cricetinae , GTP-Binding Proteins/biosynthesis , GTP-Binding Proteins/genetics , Guanosine Diphosphate , Mutagenesis , Viral Envelope Proteins/metabolism
10.
J Cell Biol ; 140(5): 1075-89, 1998 Mar 09.
Article in English | MEDLINE | ID: mdl-9490721

ABSTRACT

Stable BHK cell lines inducibly expressing wild-type or dominant negative mutant forms of the rab7 GTPase were isolated and used to analyze the role of a rab7-regulated pathway in lysosome biogenesis. Expression of mutant rab7N125I protein induced a dramatic redistribution of cation-independent mannose 6-phosphate receptor (CI-MPR) from its normal perinuclear localization to large peripheral endosomes. Under these circumstances approximately 50% of the total receptor and several lysosomal hydrolases cofractionated with light membranes containing early endosome and Golgi markers. Late endosomes and lysosomes were contained exclusively in well-separated, denser gradient fractions. Newly synthesized CI-MPR and cathepsin D were shown to traverse through an early endocytic compartment, and functional rab7 was crucial for delivery to later compartments. This observation was evidenced by the fact that 2 h after synthesis, both markers were more prevalent in fractions containing light membranes. In addition, both were sensitive to HRP-DAB- mediated cross-linking of early endosomal proteins, and the late endosomal processing of cathepsin D was impaired. Using similar criteria, the lysosomal membrane glycoprotein 120 was not found accumulated in an early endocytic compartment. The data are indicative of a post-Golgi divergence in the routes followed by different lysosome-directed molecules.


Subject(s)
Cathepsin D/metabolism , Endocytosis/physiology , GTP-Binding Proteins/metabolism , Mutagenesis , Receptor, IGF Type 2/metabolism , rab GTP-Binding Proteins , Animals , Antigens, CD/metabolism , Cathepsin D/biosynthesis , Cations , Cell Fractionation , Cell Line , Cell Membrane , Cricetinae , Endosomes/enzymology , GTP-Binding Proteins/biosynthesis , GTP-Binding Proteins/genetics , Ligands , Lysosomal Membrane Proteins , Mannosidases/metabolism , Membrane Glycoproteins/metabolism , Transfection , beta-N-Acetylhexosaminidases/metabolism , rab7 GTP-Binding Proteins
11.
Int Immunol ; 8(12): 1867-76, 1996 Dec.
Article in English | MEDLINE | ID: mdl-8982771

ABSTRACT

In B cells, processing of antigens in the context of MHC class II molecules is initiated by the binding of antigen to the B cell antigen receptor (BCR). BCR-mediated processing is highly efficient, as a consequence of the BCR's linked roles of delivering antigen to the class II peptide-loading compartment and of signaling for increased antigen-processing activity. Evidence is emerging that receptor signaling regulates intracellular transport through the activities of kinases. These in turn have been implicated in the regulation of small mol. wt GTPases which govern membrane transport. Therefore, we investigated the changes in the phosphoprotein and GTPase profiles associated with the class II peptide-loading compartment following BCR cross-linking. We first show that protein kinase inhibitors, known to block BCR signal transduction, inhibit BCR-enhanced antigen processing, demonstrating the critical dependence of enhanced processing on the signaling activity of the BCR. Consistent with this observation, the phosphoprotein profile of the class II peptide-loading compartment underwent rapid and transient changes following BCR cross-linking. We also observed a marked increase in the low mol. wt GTPases associated with the class II peptide-loading compartment within 5 min of BCR cross-linking. The observed changes in both the phosphoprotein and GTPase profiles associated with the peptide-loading compartment were blocked by kinase inhibitors and were not accompanied by overall gross changes in the protein composition of the subcellular compartments. Thus, signal cascades initiated by BCR cross-linking at the plasma membrane are translated into changes in specific subsets of regulatory proteins associated with the peptide-loading compartment.


Subject(s)
Antigen Presentation/immunology , Histocompatibility Antigens Class II/immunology , Histocompatibility Antigens Class II/metabolism , Peptides/immunology , Peptides/metabolism , Receptors, Antigen, B-Cell/immunology , Signal Transduction/immunology , Animals , Cross-Linking Reagents/chemistry , Cytochrome c Group/immunology , GTP Phosphohydrolases/immunology , Lymphoma, B-Cell , Mice , Phosphoproteins/immunology , Tumor Cells, Cultured
12.
J Biol Chem ; 271(23): 13834-43, 1996 Jun 07.
Article in English | MEDLINE | ID: mdl-8662791

ABSTRACT

Survival or destruction of a pathogen following phagocytosis depends, in part, on fusion events between the phagosome and the endosomal or lysosomal compartments. Here we use an in vitro assay to show that phagosome-endosome fusion is regulated by the small GTPase rab5 and that fusion events are influenced by an internalized live organism, Listeria monocytogenes (LM). We compare the in vitro fusion of phagosomes containing heat-killed organisms (dead LM) with that of phagosomes containing a live nonhemolytic mutant (live LMhly-). Unlike the wild-type organism, LMhly- remains trapped inside the phagosome. Phagosome-endosome fusion was reconstituted using biotinylated organisms and endosomes containing horseradish peroxidase conjugated with avidin. With both live LMhly- and dead LM preparations, in vitro phagosome-endosome fusion was time-, temperature-, and cytosol-dependent. Live LMhly- phagosomes exhibited a faster rate of fusion. Fusion in both preparations was regulated by rab5 and possibly by other GTPases. Anti-rab5 antibodies and immunodepletion of cytosolic rab5 inhibited fusion. Addition of glutatione S-transferase-rab5 in the GTP form stimulated phagosome-endosome fusion, whereas addition of a dominant negative mutant of rab5 blocked fusion. Purified live LMhly- phagosomal membranes were enriched in rab5 as revealed by Western blotting, compared with dead LM phagosomes. Fusion of endosomes with dead LM containing phagosomes required ATP and was inhibited by ATP depletion and by N-ethylmaleimide (NEM) and anti-NEM-sensitive factor (NSF) antibodies. Unexpectedly, phagosome-endosome fusion with live LMhly--containing phagosomes was not inhibited by ATP depletion nor by NEM or anti-NSF antibodies. Western blot analysis revealed that live LMhly--containing phagosomes were enriched for membrane-bound NSF, while dead LM containing phagosomes contained low or undetectable quantities. Washing live LMhly--containing phagosomes with 0.5 M KCl removed NSF associated with the membranes and rendered them NEM, ATP, anti-NSF antibody sensitive for fusion. We conclude that rab5 regulates phagosome-endosome fusion and that live microorganisms can up-regulate this process by recruiting rab5 to the membrane.


Subject(s)
Endosomes/physiology , GTP Phosphohydrolases/physiology , GTP-Binding Proteins/physiology , Listeria monocytogenes/pathogenicity , Membrane Fusion/physiology , Phagosomes/physiology , Animals , Cell Line , Endosomes/ultrastructure , GTP Phosphohydrolases/genetics , GTP-Binding Proteins/genetics , In Vitro Techniques , Kinetics , Macrophages/microbiology , Macrophages/physiology , Macrophages/ultrastructure , Microscopy, Immunoelectron , Phagocytosis/physiology , Phagosomes/ultrastructure , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , rab5 GTP-Binding Proteins
13.
J Cell Biol ; 131(6 Pt 1): 1435-52, 1995 Dec.
Article in English | MEDLINE | ID: mdl-8522602

ABSTRACT

Rab5 and rab7 proteins belong to a superfamily of small molecular weight GTPases known to be associated with early and late endosomes, respectively. The rab5 protein plays an important regulatory role in early endocytosis, yet the function of rab7 protein was previously uncharacterized. This question was addressed by comparing the kinetics of vesicular stomatitis virus (VSV) G protein internalization in baby hamster kidney cells overexpressing wild-type or dominant negative mutant forms of the rab7 protein (rab7N125I and rab7T22N). Overexpression of wild-type rab7 protein allowed normal transport to late endosomes (mannose 6-phosphate receptor positive), while the rab7N125I mutant caused the VSV G protein to accumulate specifically in early (transferrin receptor positive) endosomes. Horseradish peroxidase and paramyxovirus SV5 hemagglutinin-neuraminidase (HN) were used in quantitative biochemical assays to further demonstrate that rab7 function was not required for early internalization events, but was crucial in downstream degradative events. The characteristic cleavage of SV5 HN in the late endosome distinguishes internalization from transport to later stages of the endocytic pathway. Mutant rab7N125I or rab7T22N proteins had no effect on the internalization of either horseradish peroxidase or SV5 HN protein. In contrast, the mutant proteins markedly inhibited the subsequent cleavage of the SV5 HN protein. Taken together, these data support a key role for rab7, downstream of rab5, in regulating membrane transport leading from early to late endosomes. We compare our findings to those obtained for the yeast homologues Ypt51p, Ypt52p, Ypt53p, and Ypt7p.


Subject(s)
Endosomes/metabolism , GTP-Binding Proteins/physiology , Membrane Glycoproteins , rab GTP-Binding Proteins , Animals , Biological Transport/physiology , Biomarkers , Cell Compartmentation/physiology , Cell Line/physiology , Cell Membrane/physiology , Cricetinae , GTP-Binding Proteins/genetics , Gene Expression/physiology , Glycoproteins/metabolism , Guanosine Triphosphate/metabolism , Kidney/cytology , Lysosomes/metabolism , Mutation/physiology , Sensitivity and Specificity , Viral Envelope Proteins/metabolism , rab7 GTP-Binding Proteins
14.
J Cell Biol ; 125(3): 595-605, 1994 May.
Article in English | MEDLINE | ID: mdl-7909813

ABSTRACT

Antigen processing in B lymphocytes entails initial binding of antigen to the surface Ig and internalization of the antigen into acidic compartments where the antigen is degraded, releasing peptides for binding to major histocompatibility complex class II molecules. Using subcellular fractionation techniques we show that functional, processed antigen-class II complexes capable of activating antigen-specific T cells in vitro are first formed in dense vesicles cosedimenting with lysosomes which are distinct from early endosomes and the bulk of late endosomes. With time, processed antigen-class II complexes appear in vesicles sedimenting with early endosomes and finally cofractionate with plasma membrane. A separate compartment is identified which contains major histocompatibility complex class II receptive to peptide binding but which does not have access to processed antigen in the B cell. These class II molecules are in the so-called "floppy" form in contrast to the class II molecules in the very dense vesicles which are in the "compact" form. These results demonstrate a correlation between the floppy and compact forms of class II molecules and their association with processed antigen and show that floppy and compact forms of class II reside in distinct and physically separable subcellular compartments.


Subject(s)
Antigens, CD , Antigens, Differentiation, B-Lymphocyte , B-Lymphocytes/metabolism , Histocompatibility Antigens Class II/metabolism , Amino Acid Sequence , Animals , Antigen-Presenting Cells/immunology , Antigens/metabolism , B-Lymphocytes/immunology , B-Lymphocytes/ultrastructure , Cell Compartmentation , Endosomes/enzymology , In Vitro Techniques , Lymphocyte Activation , Lysosomal Membrane Proteins , Lysosomes/immunology , Membrane Glycoproteins/metabolism , Mice , Molecular Sequence Data , Organelles/immunology , Peptides/chemistry , Peptides/immunology , Subcellular Fractions/immunology , T-Lymphocytes/immunology
15.
Proc Natl Acad Sci U S A ; 91(11): 5061-5, 1994 May 24.
Article in English | MEDLINE | ID: mdl-8197185

ABSTRACT

In nonpolarized cells, the small GTPase Rab5a is localized to the plasma membrane, clathrin-coated vesicles, and early endosomes. Rab5a is required for early endosome fusion in vitro and regulates transport between the plasma membrane and early endosomes, in vivo. In polarized epithelial cells endocytosis occurs from separate apical and basolateral plasma membrane domains. Internalized molecules are initially delivered to distinct apical or basolateral early endosomes. In vitro, apical early endosomes can readily fuse with one another but not with the basolateral endosomes and vice versa, thereby indicating that the apical and basolateral early endocytic pathways are controlled by distinct machineries. Here, we have investigated the localization and function of Rab5a in polarized epithelial cells. Confocal immunofluorescence microscopy on mouse kidney sections revealed association of the protein with the apical and basolateral plasma membrane domains and underlying structures. In polarized Madin-Darby canine kidney I cells, endogenous and overexpressed Rab5a have the same distribution. Moreover, overexpression of the protein causes a 2-fold increase in fluid-phase uptake from both domains of the cell, thus showing that Rab5a functions in apical and basolateral endocytosis. Our data indicate that the apical and basolateral endocytic machineries of epithelial cells share common regulatory components and that Rab5a per se is not sufficient to target endocytic vesicles to apical or basolateral early endosomes.


Subject(s)
Endocytosis , GTP-Binding Proteins/metabolism , Amino Acid Sequence , Animals , Biological Transport , Cell Line , Dogs , Epithelial Cells , Fluorescent Antibody Technique , GTP Phosphohydrolases/metabolism , Molecular Sequence Data , rab5 GTP-Binding Proteins
16.
J Cell Biol ; 111(3): 987-1000, 1990 Sep.
Article in English | MEDLINE | ID: mdl-2202740

ABSTRACT

Immunoisolation techniques have led to the purification of apical and basolateral transport vesicles that mediate the delivery of proteins from the trans-Golgi network to the two plasma membrane domains of MDCK cells. We showed previously that these transport vesicles can be formed and released in the presence of ATP from mechanically perforated cells (Bennett, M. K., A. Wandinger-Ness, and K. Simons, 1988. EMBO (Euro. Mol. Biol. Organ.) J. 7:4075-4085). Using virally infected cells, we have monitored the purification of the trans-Golgi derived vesicles by following influenza hemagglutinin or vesicular stomatitis virus (VSV) G protein as apical and basolateral markers, respectively. Equilibrium density gradient centrifugation revealed that hemagglutinin containing vesicles had a slightly lower density than those containing VSV-G protein, indicating that the two fractions were distinct. Antibodies directed against the cytoplasmically exposed domains of the viral spike glycoproteins permitted the resolution of apical and basolateral vesicle fractions. The immunoisolated vesicles contained a subset of the proteins present in the starting fraction. Many of the proteins were sialylated as expected for proteins existing the trans-Golgi network. The two populations of vesicles contained a number of proteins in common, as well as components which were enriched up to 38-fold in one fraction relative to the other. Among the unique components, a number of transmembrane proteins could be identified using Triton X-114 phase partitioning. This work provides evidence that two distinct classes of vesicles are responsible for apical and basolateral protein delivery. Common protein components are suggested to be involved in vesicle budding and fusion steps, while unique components may be required for specific recognition events such as those involved in protein sorting and vesicle targeting.


Subject(s)
Kidney/cytology , Membrane Glycoproteins , Membrane Proteins/metabolism , Organelles/metabolism , Animals , Biological Transport/physiology , Cell Fractionation , Cell Membrane/metabolism , Dogs , Electrophoresis, Gel, Two-Dimensional , Hemagglutinin Glycoproteins, Influenza Virus , Hemagglutinins, Viral/metabolism , Immunologic Techniques , In Vitro Techniques , Kidney/metabolism , Viral Envelope Proteins/metabolism
20.
EMBO J ; 7(13): 4075-85, 1988 Dec 20.
Article in English | MEDLINE | ID: mdl-3243273

ABSTRACT

Mechanically perforated MDCK cells were used to study membrane transport between the trans-Golgi network and the apical and basolateral plasma membrane domains in vitro. Three membrane transport markers--an apical protein (fowl plague virus haemagglutinin), a basolateral protein (vesicular stomatitis virus G protein), and a lipid marker destined for both domains (C6-NBD-sphingomyelin)--were each accumulated in the trans-Golgi by a 20 degrees C block of transport and their behaviour monitored following cell perforation and incubation at 37 degrees C. In the presence of ATP and in the absence of calcium ions a considerable fraction of the transport markers were released from the perforated cells in sealed membrane vesicles. Control experiments showed that the vesicles were not generated by non-specific vesiculation of the Golgi complex or the plasma membrane. The vesicles had well defined sedimentation properties and the orientation expected of transport vesicles derived from the trans-Golgi network.


Subject(s)
Cell Membrane/metabolism , Exocytosis , Animals , Biological Transport, Active , Biomarkers , Cell Line , Centrifugation, Density Gradient , Golgi Apparatus/metabolism , Intracellular Membranes/metabolism
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