Chiral carbon dots derived from tryptophan and threonine for enantioselective sensing of L/D-Lysine.

Presently, most fluorescent probes for amino acid enantiomers detection require metal ions participation, which greatly increases the detection steps and costs, and affects the accuracy of detection results. To solve this problem, a dual pattern recognition sensor of chiral carbon dots (L-Try-Thr-CDs) with a quantum yield of 36.23 % was prepared by a one-step solvothermal method for the highly selective detection of lysine (Lys) enantiomers. Under optimal experimental conditions, the fluorescence and circular dichroism (CD) signals of the obtained L-Try-Thr-CDs could rapidly and effectively responded to L-Lys with limits of detection (LOD) of 16.51 nM and 24.38 nM, respectively, much lower than previously reported sensors. Importantly, the L-Try-Thr-CDs as a dual-mode sensor could not only detect amino acid enantiomers and simplify the steps, but also avoid inaccurate detection results due to unstable metal ions. Furthermore, the L-Try-Thr-CDs could detect L-Lys in living cells via a fluorescence microscope because of their excellent fluorescence characteristics and low toxicity. These results indicated that the dual-mode sensor not only provided a practical strategy for the design of new fluorescent probes, but also possessed outstanding application prospects in the accurate detection of lysine enantiomers.

Nitrogen-doped graphene quantum dot-based portable fluorescent sensors for the sensitive detection of Fe3+ and ATP with logic gate operation.

Adenosine triphosphate (ATP) and Fe3+ are important "signaling molecules" in living organisms, and their abnormal concentrations can be used for the early diagnosis of degenerative diseases. Therefore, the development of a sensitive and accurate fluorescent sensor is essential for detecting these signaling molecules in biological matrices. Herein, nitrogen-doped graphene quantum dots (N-GQDs) with cyan fluorescence emission were prepared by thermal cleavage of graphene oxide (GO) with N,N-dimethylformamide (DMF) as a solvent. The synergistic effect of static quenching and internal filtration enabled the selective quenching of N-GQD fluorescence by Fe3+. With the introduction of ATP, Fe3+ in the N-GQDs-Fe3+ system formed a more stable complex with ATP via the Fe-O-P bond, thus restoring the fluorescence of the N-GQDs. Fe3+ and ATP were detected in the linear ranges of 0-34 µM and 0-10 µM with the limits of detection (LOD) of 2.38 nM and 1.16 nM, respectively. In addition to monitoring Fe3+ and ATP in mouse serum and urine, the proposed method was also successfully applied for cytoplasmic imaging of 4T1 cells and in vivo imaging of freshwater shrimps. Moreover, the fluorescence and solution color change-based "AND" logic gate was successfully demonstrated in the biological matrix. Importantly, a complete sensing system was constructed by combining the N-GQDs with hydrogel kits and fluorescent flexible films. Thus, the prepared N-GQDs can be expected to serve as a valuable analytical tool for monitoring Fe3+ and ATP concentrations in biological matrices.

Ionic liquid-based carbon dots as highly biocompatible and sensitive fluorescent probe for the determination of vitamin P in fruit samples.

Vitamin P (VP) known as rutin is one of the common flavonoids, which widely exists in fruits and vegetables and often used as a dietary additive. The rapid and accurate detection of VP in food matrices is critical for evaluating food quality and guiding diet. Herein, a rapid, accurate, and selective detection scheme for VP in fruit samples was proposed for the first time using ionic liquid-based carbon dots (IL-CDs). The synthesized IL-CDs exhibited great biocompatibility and excellent optical properties including high fluorescence intensity, high quantum yield, and good fluorescence stability. Through an internal filtering effect (IFE), VP could greatly reduce the fluorescence of these CDs. In the present study, this probe demonstrated good sensitivity and excellent selectivity toward VP with a low detection limit of 60.0 nmol/L. Moreover, this approach was effectively applied to detect VP in food samples with a recovery range of 97 % to 119 %. More interestingly, the results of cell imaging suggested that IL-CDs were expected to be promising material for bioimaging.

Facile synthesis of nitrogen-doped carbon dots as sensitive fluorescence probes for selective recognition of cinnamaldehyde and l-Arginine/l-Lysine in living cells.

The disorder of amino acid metabolism and the abuse of small molecule drugs pose serious threats to public health. However, due to the limitations of existing detection technologies in sensing cinnamaldehyde (CAL) and l-Arginine/l-Lysine (l-Arg/l-Lys), there is an urgent need to develop new sensing strategies to meet the severe challenges currently facing. Herein, nitrogen-doped carbon dots (N-CDs) were developed using a simple one-pot hydrothermal carbonization method. These N-CDs exhibited numerous distinctive characteristics such as excellent photoluminescence, high water dispersibility, favorable biocompatibility, and superior chemical inertness. Strikingly, the as-prepared CDs as a highly efficient fluorescent probe possessed significant sensitivity and selectivity toward CAL and l-Arg/l-Lys over other analytes with a low detection limit of 58 nM and 16 nM/18 nM, respectively. The fluorescence of N-CDs could be quenched by CAL through an electron transfer process. Then, the strong electrostatic interaction between l-Arg/l-Lys and N-CDs induced the efficient fluorescence recovery. More importantly, the outstanding biosafety and excellent analyte-responsive fluorescence characteristics of N-CDs have also been verified in living cells as well as in serum and urine. Overall, the N-CDs had a wide application prospect in the diagnosis of amino acid metabolic diseases and small molecule drug sensing.