Microsurgical reconstruction for head and neck in patients with end-stage renal disease undergoing dialysis.

INTRODUCTION: Free flap transfer for head and neck defects has gained worldwide acceptance. Because flap failure is a devastating outcome, studies have attempted to identify risk factors-including renal failure. We sought to determine whether end-stage renal disease (ESRD) patients undergoing dialysis are at increased risk of flap failure following microsurgical head and neck reconstruction. PATIENTS AND METHODS: The study's participants were patients who underwent free flap reconstruction in the head and neck region at Hualien Tzu Chi Hospital between January 2010 and December 2019. We used the National Health Insurance "Specific Diagnosis and Treatment Code" to identify patients undergoing dialysis; these patients comprised the dialysis group, whose members were matched to a non-dialysis group for age and gender. The dependent variables were flap survival rate, take-back rate, and flap failure risk between the dialysis and non-dialysis groups. RESULTS: We included 154 patients in the dialysis (n = 14) and non-dialysis (n = 140) groups. The groups were similar in terms of age and most comorbidities, except diabetes mellitus, hypertension, and coronary artery disease, which were more prevalent in the dialysis group. The dialysis and non-dialysis groups had similar flap survival rates (100% vs. 92.9%; p = .600). Twenty-three patients underwent take-back surgery, most in the non-dialysis group (14.3% vs. 15.0%; p = 1.000). Patients in the dialysis group were more likely to have prolonged intensive care unit stays; however, dialysis alone did not predict flap failure (OR: 0.83; p = .864). CONCLUSION: This study found no significant differences in free flap survival and take-back rates between patients with and without dialysis. Dialysis did not increase the risk of flap failure following microsurgical head and neck reconstruction in this study; however, prospective, randomized controlled trials are needed.

Dermoscopic features of pigmented basal cell carcinoma according to size.

BACKGROUND: Basal cell carcinoma (BCC), the most common skin cancer in humans, requires early detection. Dermoscopy enhances diagnostic accuracy through a noninvasive approach. Pigmented BCC (pBCC) is characterized by distinctive dermoscopic features, including the presence of pigmented globules or nests. While dermoscopic features of large pBCC (size >6 mm) have been extensively studied, limited data are available on small pBCC (size ≤6 mm) and its relationship with tumor progression. METHODS: Dermoscopic images of histologically proven pBCCs were collected between 2014 and 2022 at Hualien Tzu Chi Hospital. Each image was analyzed for patterns of pigmentation, vasculature, and epidermal and dermal structures. Statistical analysis was performed to compare the differences according to the size and the trend during tumor progression. RESULTS: In total, 135 pBCCs (48 small and 87 large) were included. Pigment structures were present in all cases. Short fine telangiectasia and small erosions constituted over 85% of the cases, showing no significant distinction between small and large pBCCs, nor any specific pattern correlating with tumor enlargement. The number of arborizing vessels, ulcerations, and shiny white structures showed an increasing trend associated with size progression. Arborizing vessels appeared when tumor size exceeded 6 mm. CONCLUSIONS: This study provides a dynamic interpretation of the dermoscopic features of pBCC according to size enlargement. Short fine telangiectasia and small erosions are highly important features for the early diagnosis of small pBCCs. Arborizing vessels, ulceration, and shiny white structures are more frequent in large pBCCs, and they increase in association with tumor progression.

In vivo assessment of endothelial function in small animals using an infrared pulse detector.

OBJECTIVE: Endothelial dysfunction is the earliest change in atherosclerosis. Flow-mediated dilatation (FMD) is used to assess endothelial function in humans. However, this assessment is not easy in small animals. This study demonstrated the reliability and reproducibility of a proposed instrument for in vivo assessment of FMD in a rodent model using infrared pulse sensors. MATERIALS AND METHODS: We used 24 adult male Wistar Kyoto rats randomly divided into three groups. FMD was measured under continuous infusion of normal saline followed by intra-arterial infusion of acetylcholine (Ach; n = 8), sodium nitroprusside (SNP; n = 8), or Nω-nitro-L-arginine methyl ester (L-NAME; n = 8). RESULTS: The dilatation indices (DIs) of all three groups were similar before application of the vasoactive agents (1.82 ± 0.46, 1.81 ± 0.44, and 1.93 ± 0.40, P = 0.877, by one-way analysis of variance). The DI was significantly increased during infusion of Ach (2.97 ± 1.03 vs. 1.82 ± 0.46, P = 0.015), unchanged during infusion of SNP (1.81 ± 0.44 vs. 1.98 ± 0.40, P = 0.574), and attenuated during infusion of L-NAME (1.91 ± 0.40 vs. 1.42 ± 0.35; P = 0.028). CONCLUSION: The results of this study correlated well with those of human studies, suggesting that this method can be used for in vivo evaluation of endothelial function in small animals.

Bathtub drowning mortality among older adults in Japan.

We examined the bathtub drowning mortality among older adults in Japan. Mortality data from Japan and 30 other Organization for Economic Co-operation and Development (OECD) countries were extracted from World Health Organization Cause of Death Query Online. During 2012-2014, unintentional drowning mortality rates in Japan were 9.5, 28.2 and 39.7 per 100,000 population for adults aged 65-74, 75-84 and ≥85 years, respectively-rates highest among the 31 OECD countries. In total, 6377 older adults aged ≥65 years died from unintentional drowning in 2014, of which 4857 (76%) deaths involved bathtubs. During 1995-2014, the bathtub drowning mortality rate for adults aged ≥65 years was stable in Japan. During 2011-2014, approximately 4800 older adults died from bathtub drowning annually. Death predominantly occurred 'while in a bathtub', rather than 'following a fall into a bathtub'. In 2014, 95% and 87% of bathtub drowning deaths among older women and men aged ≥65 years, respectively, occurred at home. In conclusion, bathtub drowning deaths at home is an important public health problem among older adults Japanese and efforts are needed to reduce these preventable deaths.

Drowning mortality by intent: a population-based cross-sectional study of 32 OECD countries, 2012-2014.

OBJECTIVE: To compare the drowning mortality rates and proportion of deaths of each intent among all drowning deaths in Organisation for Economic Co-operation and Development (OECD) countries in 2012-2014. DESIGN: A population-based cross-sectional study. SETTING: 32 OECD countries. PARTICIPANTS: Individuals in OECD countries who died from drowning. MAIN OUTCOME MEASURES: Drowning mortality rates (deaths per 100 000 population) and proportion (%) of deaths of each intent (ie, unintentional intent, intentional self-harm, assault, undetermined intent and all intents combined) among all drowning deaths. RESULTS: Countries with the highest drowning mortality rates (deaths per 100 000 population) were Estonia (3.53), Japan (3.49) and Greece (2.40) for unintentional intent; Ireland (0.96), Belgium (0.96) and Korea (0.89) for intentional self-harm; Austria (0.57), Korea (0.56) and Hungary (0.44) for undetermined intent and Japan (4.35), Estonia (3.70) and Korea (2.73) for all intents combined. Korea ranked 12th and 3rd for unintentional intent and all intents combined, respectively. By contrast, Belgium ranked 2nd and 15th for intentional self-harm and all intents combined, respectively. The proportion of deaths of each intent among all drowning deaths in each country varied greatly: from 26.2% in Belgium to 96.8% in Chile for unintentional intent; 0.7% in Mexico to 57.4% in Belgium for intentional self-harm; 0.0% in nine countries to 4.9% in Mexico for assault and 0.0% in Israel and Turkey to 38.3% in Austria for undetermined intent. CONCLUSIONS: A large variation in the practice of classifying undetermined intent in drowning deaths across countries was noted and this variation hinders valid international comparisons of intent-specific (unintentional and intentional self-harm) drowning mortality rates.

Using matrix frame to present road traffic injury pattern.

BACKGROUND: Although many epidemiological studies have presented road traffic injuries (RTIs) according to the victim's mode of transport, very few have mentioned the mode of transport of the victim's counterparts. We sought to use matrix frame to present the pattern of RTIs based on the International Classification of Diseases, Tenth Revision (ICD-10) codes. METHODS: Patients admitted to Hualien Tzu Chi Hospital, Taiwan, for RTIs from January 1, 2013 to December 31, 2016 were included. The numbers and proportions of various crash types of RTIs were presented using a matrix frame. The row margin of the matrix is the second character of ICD-10 codes V00-V79 (victim's mode of transport), and the column margin of the matrix is the third character of ICD-10 codes V00-V79 (mode of transport of victim's counterpart), constituting a 80-cell grid. RESULTS: In total, 2727 patients were included. The cell with the highest proportion in the matrix grid was ICD-10 code V23 "motorcycle rider injured in collision with car, pick-up truck or van" (27.0%, 737/2727), followed by that of V27 "motorcycle rider injured in collision with fixed or stationary object" (12.5%, 342/2727) and V28 "motorcycle rider injured in noncollision transport accident" (12.2%, 334/2727). The matrix pattern of RTIs differed with sex and age. CONCLUSIONS: By using the matrix frame, we can easily understand the RTI pattern for different demographic groups and identify the priority crash types.

Ranking regional drowning mortality rates: population-based versus aquatic area-based rate.

Population, the most often used denominator in calculating drowning mortality rate, might not be a good measure of risk exposure. In this study, we sought to compare the rankings of township drowning mortality according to population-based rates with those according to aquatic area-based rates in Taitung County, Taiwan. The township with the highest mortality rate using population as the denominator (deaths per 100 000 persons) was Changbin (32.3) followed by Lanyu (22.4), Donghe (19.6) and Chenggong (18.0). On the other hand, the township with the leading mortality rate using aquatic area as the denominator (deaths per 100 000 km2) was Lanyu (353) followed by Lyudao (307), Chenggong (282) and Taitung City (219). Taitung City ranked 10th according to the population-based rate, but ranked 4th according to the aquatic area-based rate. In conclusion, rankings of regional drowning mortality rates using aquatic area as the denominator differ from those using population as the denominator.

A comparison between proximal lateral leg flap and radial forearm flap for intraoral reconstruction.

Free flaps have become a popular option for the reconstruction of intraoral defects. The radial forearm flap used to be the workhorse flap for small and thin defects, but was associated with numerous donor-site morbidities. The proximal lateral leg flap can provide a thin and pliable tissue similar to the radial forearm flap but without the related donor-site morbidities. We compared the differences between these 2 flaps. Thirty-four patients with intraoral defects from September 2005 to October 2011 were reconstructed, using the radial forearm flap in 23 cases, and the proximal lateral leg flap in 11 cases. The radial forearm flap group had a success rate of 95.6%. The flap survival rate was 100% in the proximal lateral leg flap group. However, the difference was statistically insignificant. Skin graft was required in 22 of the 23 cases for the donor site of the radial forearm flap. Partial loss of the skin graft occurred in 5/22 (23%) of the patients, with exposure of tendons in 3/22 (14%). Delay in healing of the donor sites occurred in 6/23 (26%) of the patients. The donor sites of the proximal lateral leg flap were all closed primarily. One case developed wound dehiscence and this healed by conservative treatment. Long-term follow-up showed functional impairment of the donor forearm (reduced extension or grip strength) in 17% of the patients. Thirty percent of the patients developed sensory disturbance and 48% complained of poor outcome of the donor forearms. In the proximal lateral leg flap group, no motor or sensory functional deficits were seen. No patients complained of poor outcome of the donor legs. Primary closure of the donor site of the proximal lateral leg flap could be performed if the flap width was less than 6 cm. This flap is useful for patients with small and thin intraoral defects and is associated with minimal donor-site morbidity when compared to the radial forearm flap.

Using adenovirus armed short hairpin RNA targeting transforming growth factor ß1 inhibits melanoma growth and metastasis in an ex vivo animal model.

The transforming growth factor ß (TGF-ß) is the key molecule implicated in impaired immune function in human patients with malignant melanoma. TGF-ß can promote tumor growth, invasion, and metastasis in advanced stages of melanoma. Blocking these tumor-promoting effects of TGF-ß provides a potentially important therapeutic strategy for the treatment of melanoma. In this study, we used an adenovirus-based shRNA expression system and successfully constructed Ad/TGF-ß1-RNA interference (RNAi) which mediated the RNAi for TGF-ß1 gene silencing. We examined the effects of TGF-ß1 protein knockdown by RNAi on the growth and metastasis of melanoma in C57BL/6 mice induced by the B16F0 cell line. The TGF-ß1 hairpin oligonucleotide was cloned into adenoviral vector. The resulting recombinant adenoviruses infected murine melanoma cell line, B16F0, and designated as B16F0/TGF-ß1-RNAi cells. The blank adenoviral vector also infected B16F0 cells and designed as B16F0/vector-control cells served as a control. TGF-ß1 expression was reduced in B16F0/TGF-ß1-RNAi cells compared with B16F0 cells and B16F0/vector-control cells. Three million wild-type B16F0 cells, B16F0/vector-control cells, and B16F0/TGF-ß1-RNAi cells were injected subcutaneously into the right flanks of adult female syngeneic mice C57BL/6. The tumor sizes were 756.09 (65.35), 798.48 (78.77), and 203.55 (24.56) mm at the 14th day in the mice receiving B16F0 cells, B16F0/vector-control cells, and B16F0/TGFß1-RNAi cells, respectively. The P value was less than 0.01 by 1-way analysis of variance. TGF-ß1 knockdown in B16F0 cells enhanced the infiltration of CD4 and CD8 T cells in the tumor regions. C57BL/6 mice were evaluated for pulmonary metastasis after tail vein injection of 2 million B16F0 cells, B16F0/vector-control cells, and B16F0/TGF-ß1-RNAi cells. The pulmonary metastasis also reduced significantly on days 14 day and 21 in mice injected with B16F0/TGF-ß1-RNAi tumors. The blood vessel density of the tumors markedly reduced in B16F0/TGF-ß1-RNAi tumors. Our results showed that Ad/TGF-ß1-RNAi could induce silencing of the TGF-ß1 gene effectively. Silencing of TGF-ß1 expression in B16F0 cells by RNAi technology can inhibit the growth and metastasis of this tumor after being transplanted to C57BL/6 mice. This kind of adenoviral vector based on RNAi might be a promising vector for cancer therapy.

Simultaneous assessment of autonomic nervous and vascular endothelial functions in a rat model.

Despite the importance of translational medicine in cardiovascular research, rat vascular endothelial function and sympathetic activity assessment remains challenging.This study consisted of two parts: part A examined the consistency of reactive hyperemia-elicited dilatation index(DI) before (DI Original ) and after (DI EEMD ) ensemble empirical mode decomposition (EEMD) using Wistar-Kyoto (WKY)rats (n = 7). Part B compared the endothelial function and heart rate variability of normal WKY rats (group 1, n = 9)with those of spontaneously hypertensive rats (group 2,n = 9) at two time points of development. The SD1/SD2 ratio(SSR) was obtained from Poincaré plot analysis to evaluate sympathetic activities. Using a Bland-Altman plot, part A of the study demonstrated a lower agreement of DI Original than that of DI EEMD . In part B, despite no significant difference in DI EEMD between the two groups at age 10 weeks, DI EEMD was higher in group 1 (2.25 } 0.63 vs. 1.43 } 0.41 for groups 1 and 2, respectively, p = 0.004) at 20 weeks. Although no notable change in SSR existed in group 1 between the twotime points, a significant difference existed in group 2(p < 0.001). In conclusion, using algorithms of Poincaré plot analysis and EEMD, the impact of age and hypertension on rat vascular endothelial function and heart rate variability can be reliably assessed.

Expanding the applications of the pedicled anterolateral thigh and vastus lateralis myocutaneous flaps.

We describe our experience in expanding the use of pedicled anterolateral thigh and vastus lateralis myocutaneous flaps. A total of 33 patients underwent 34 flaps between March 2003 and January 2012. The defects included 18 ischial, 3 trochanteric, 5 lower abdomen, 2 perineogenital, 1 groin, and 5 knee defects. There were 29 proximal pedicled (2 of which were preexpanded), 3 distal pedicled, and 2 propeller flaps. Complications included 1 total necrosis, 1 partial necrosis, 3 wound dehiscence in recipient site, 1 hematoma, and 1 donor-site dehiscence. The total flap survival rate was 94%. There were no donor-site morbidities except poor cosmesis in the skin-grafted sites. Pedicled anterolateral thigh flap is highly versatile with a wide arc of rotation. A proximal pedicled flap can be used for lower abdomen, perineogenital, ischial, and trochanteric defects and the distal pedicled or a propeller flap for knee and proximal lower leg defects.

Reconstruction of extensive composite oromandibular defects with simultaneous free anterolateral thigh fasciocutaneous and fibular osteocutaneous flaps.

Massive composite defects of the face are difficult to reconstruct. Such defects are usually created after ablation of advanced cancers of the head and neck region. The use of a free fibular osteocutaneous flap for the bone and mucosal lining of the oral cavity and anterolateral thigh flap for the outer cutaneous lining are well established. We present our experience of using these two flaps simultaneously in the reconstruction of such defects and to evaluate the outcome. There were a total of 10 patients in our study. Their average age was 48.8 years. All had squamous cell carcinoma of the oral cavity. Their pathological stages were mostly stage T4 with only one case being T3. Flap survival was 100%. The application of dual free flaps, though technically more demanding, allows good orientation of the flaps. Seven patients maintained a good functional outcome. They were able to eat a soft diet. Their speech was easily comprehensible. The combination of a free anterolateral thigh flap with vascularized fibular osteocutaneous flap can be performed safely with adequate functional outcome. This combination of flaps should be considered for this group of patients.

A non-invasive assessment for endothelial function of small animals.

At present, many studies indicate that atherosclerosis has high correlation with risk factors of cardiovascular diseases. Furthermore, atherosclerosis usually accompanies with endothelial dysfunction. However, endothelial dysfunction dose not necessarily have situation of atherosclerosis. It means that endothelial dysfunction can be the early change of atherosclerosis. If we can use non-invasive methods to monitor the function of vascular endothelia, it will be very helpful for early assessment of atherosclerosis. It can also detect atherosclerosis earlier then decrease the mortality and mobility of atherosclerosis related diseases, such as coronary artery disease, stroke and chronic renal disease, with appropriate management.

A new technique of transferring island pedicled anterolateral thigh and vastus lateralis myocutaneous flaps for reconstruction of recurrent ischial pressure sores.

We describe island pedicled anterolateral thigh and vastus lateralis myocutaneous flaps for reconstruction of the difficult, recurrent ischial pressure sore. Rather than transfer through a subcutaneous tunnel, the flap is transferred directly through the upper thigh to the ischial defect. A total of 15 patients with 16 recurrent ischial pressure sores were treated between May 2003 and April 2005. Eleven sores were treated with pedicled island anterolateral thigh flaps and five sores with vastus lateralis myocutaneous flaps. There was no difficulty in transferring the flap to reach the ischial defect in any patient. The length of the pedicle ranged from 8.5 to 14 cm. All donor sites were closed primarily. Fifteen of the 16 flaps survived completely. Total necrosis occurred in one vastus lateralis myocutaneous flap, which was located at the distal third of the thigh. We conclude this flap can be added to the repertoire for the treatment of recurrent, difficult ischial pressure sores.

Subtotal resection of gastrocnemius muscles for hypertrophic muscular calves in Asians.

BACKGROUND: Many Asian women are bothered by plump calves, which they consider to be unsightly. In the past, liposuction was frequently performed to treat oversized legs, but little effect could be obtained if the condition was caused by hypertrophy of calf muscles. METHODS: Gastrocnemius muscle resection for hypertrophic muscular calves was performed in 20 patients at Buddhist Tzu Chi General Hospital from May of 2001 through September of 2004. One patient underwent resection of the medial head only, two underwent total resection of both medial and lateral heads, and the other 17 patients underwent subtotal resection with preservation of the proximal 4 to 6 cm of muscle of both heads. RESULTS: The resected gastrocnemius muscle weighed from 250 to 430 g per calf (mean, 343 g). The maximal calf circumference was between 32 and 41 cm (average, 35.2 cm) preoperatively and 26 and 35 cm (average, 30.5 cm) postoperatively. Calf reduction was 3.5 to 7 cm (average, 4.7 cm), or 10.1 to 21.2 percent (average, 13.3 percent). Two patients developed seroma and three developed late hematoma. One patient had dragging of one foot because of incidental severance of the nerve to the soleus muscle that lasted for 6 months, until complete recovery occurred. No patient complained of functional deficits during gait or sports activities after 3 to 6 months. CONCLUSIONS: Subtotal resection of both heads of the gastrocnemius muscle can result in satisfactory calf reduction without impairment of leg function and avoid hollow deformity in the popliteal fossa. Five patients still had scar problems during the short-term follow-up period, but all were rather satisfied with their reduced calf sizes.

Experience with elastic rubber bands for the tie-over dressing in skin graft.

We derived a dressing using elastic rubber bands to tie over the skin graft. This is a simple, easy to perform, timesaving, inexpensive and reliable method for applying pressure over the skin graft compared with traditional methods. Between September 2002 and August 2004, we have used the present dressing technique in 35 patients with 36 grafts in various parts of the body. We chose this method, because of some anatomic areas, such as back, and buttock, which are frequently quite difficult to maintain pressure dressings in place, minimal movement can cause the skin graft to dislodge. The elastic rubber bands, rather than threads, are used as tie-over. Such a dressing permits expansion and contraction, providing a dynamic quality in the most difficult anatomic locations. The patient group consisted of 23 males and 12 females. The age ranged from 34 to 82 years (mean 52.4 years). Defect size ranged from 3x2.5 to 30x20 cm2 (mean 11.2x7.0 cm2 in size). The average follow-up was 5.8 months (range: 1-12 months). Among the 36 grafts in our study, all grafts except four showed good to excellent results. The mean graft successful rate is 88%. With our procedure no hematoma formation or shearing force (except one case) occurred in this group of patients during the phase of revascularization, there was, hence, good fixation of the graft by the "tie-over" dressing using elastic rubber bands compared with conventional tie-over dressing, especially in skin grafts of the back site of body and at large graft area. However, it is not suitable for the potentially infectious granulation beds, especially near joint area.

Reconstruction of trunk defects with bilobed myocutaneous flap.

This study describes the use of the bilobed myocutaneous (MC) flap for reconstruction of trunk defect. The authors employ this flap because it offers a greater flexibility in the use of available tissue than many other local conventional flaps, is easy to design, safe to elevate, and causes minimal donor-site morbidity. The bilobed MC flaps are based on their main muscular pedicle. The standard bilobed flaps are generally based on a random pattern blood supply, which were restricted to rigid length-to-width ratios to ensure viability. They are usually indicated in the repair of small defects of the nose, and scalp. When we want to apply to reconstruct on the larger trunk defect, especially for the potential to cause life-threatening complications, the bilobed M-C flap might be considered for reconstruction to decrease the potential risk of the complications.