ABSTRACT
Considering the general application of dedicated small-animal positron emission tomography/computed tomography is limited, an acceptable alternative in many situations might be clinical PET/CT. To estimate the feasibility of using clinical PET/CT with [F-18]-fluoro-2-deoxy-D-glucose for high-resolution dynamic imaging and quantitative analysis of cancer xenografts in nude mice. Dynamic clinical PET/CT scans were performed on xenografts for 60 min after injection with [F-18]-fluoro-2-deoxy-D-glucose. Scans were reconstructed with or without SharpIR method in two phases. And mice were sacrificed to extracting major organs and tumors, using ex vivo γ-counting as a reference. Strikingly, we observed that the image quality and the correlation between the all quantitive data from clinical PET/CT and the ex vivo counting was better with the SharpIR reconstructions than without. Our data demonstrate that clinical PET/CT scanner with SharpIR reconstruction is a valuable tool for imaging small animals in preclinical cancer research, offering dynamic imaging parameters, good image quality and accurate data quatification.
ABSTRACT
In the present study, an arbuscular mycorrhizal fungus (AMF) strain Acaulospora spinosa XJ27, previously isolated from tomato root rhizosphere in Xinjiang' s suburbs, was used to inoculate root systems of tomato cultivar "Micro-Tom". The biomass, contents of chlorophyll in leaves and lycopene in fruits of plants between the treatments with and without XJ27 were analyzed, and the expression level of lycopene biosynthesis relevant genes (psyl and.psy2) investigated by semi quantitative-PCR and real-time PCR. The results indicated that the application of XJ27 to the rhizosphere of tomato increased the biomass and yield of the treated plants, the expression of psyl and psy2 was improved, and the amount of lycopene accumulation in tomato. It suggested Acaulospora spinosa XJ27 has a great potential in utilization.
