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Zhong Yao Cai ; 30(2): 213-6, 2007 Feb.
Article in Chinese | MEDLINE | ID: mdl-17571774

ABSTRACT

OBJECTIVE: To optimize the extraction condition for the polysaccharides from Marasmius androsaceus Mycelium. METHODS: The single factor tests and response surface methodology (RSM) were applied. Overall research was proceeded by rational desingn. Technological condition parameters were determined according to regression analysis by SAS. RESULTS: The optimum condition was as follows: extraction temperature was 78 degrees C , extraction-duration was 1. 5h, water to material ratio was 36: 1. The optimum extracting rate for the polysaccharide was 11. 59%. CONCLUSION: The RSM is for optimum extraction process and it will improve the extraction conditions of polysaccharides.


Subject(s)
Agaricales/chemistry , Drugs, Chinese Herbal/isolation & purification , Polysaccharides/isolation & purification , Technology, Pharmaceutical/methods , Drugs, Chinese Herbal/analysis , Drugs, Chinese Herbal/chemistry , Mycelium/chemistry , Polysaccharides/analysis , Regression Analysis , Spectrophotometry, Ultraviolet , Temperature , Time Factors , Water
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