Naproxen attenuates osteoarthritis progression through inhibiting the expression of prostaglandinl-endoperoxide synthase 1.

OBJECTIVE: This study aims to test the effect of naproxen treatment and the biological target of naproxen for treating osteoarthritis (OA). METHODS: Differentially expressed genes (DEGs) in OA synovial tissues and normal counterparts were analyzed by messenger RNA microarray analysis. R package (weighted gene coexpression network analysis) was used to divide DEGs into several modules and determine the hub genes in each module. The expression level of prostaglandin-endoperoxide synthase 1 ( PTGS1) in OA synovial cells and tissues was verified by a quantitative real-time polymerase chain reaction and western blot. Transwell assay evaluated the numbers of cell migration and invasion. Furthermore, Safranin O and fast green staining and hematoxylin and eosin staining were performed on joints from anterior cruciate ligament transection mice. RESULTS: Microarray analysis determined PTGS1 was the hub gene in the black module, which was overexpressed in OA synovial cells and tissues compared with normal synovial cells. OA synovial cells transfected with sh-PTGS1 showed downregulation of PTGS1. After treatment with naproxen, the expression of PTGS1 sharply decreased in the OA group. The migration and invasion of OA synovial cells increased, whereas the cell apoptosis rate decreased when PTGS1 was overexpressed. However, the cell migration and invasion decreased, whereas cells apoptosis increased when it was treated with naproxen. Naproxen could also influence the expression level of six OA-related genes: LUBRICIN, matrix metalloproteinase 13 (MMP-13), cyclooxygenase-2 (COX-2), ACAN, COL2A1, and COL1A1. CONCLUSION: We validated that naproxen could suppress the expression of PTGS1 in synovial cells. Moreover, naproxen could inhibit the migration/invasion ability of OA synoviocytes and promote the apoptosis rate OA synoviocytes.

Association between dietary patterns and depressive symptoms among middle-aged adults in China in 2016-2017.

The purpose of this study was to explore the associations of dietary patterns with depressive symptoms in middle-aged Chinese adults. The cross-sectional study participants were 1360 Chinese adults (45-59 years, 659 males and 701 females) who participated in a Health Survey at the time of periodic checkup. Dietary intakes were assessed via a semi-quantitative food frequency questionnaire (FFQ). Binary logistic regression analysis was used to estimate odds ratio(OR) and 95% confidence interval(CI) for depressive symptoms according to quartiles of each dietary pattern score. Four major dietary patterns were identified by factor analysis: traditional Chinese, Western, grains-vegetables and high-salt patterns. After controlling for potential confounders, participants in the highest quartile of the Western pattern scores had greater odds of depressive symptoms than those in the lowest quartile. In contrast, participants in the highest quartile of the grains-vegetables pattern had lower odds of depressive symptoms than those in the lowest quartile. Nevertheless, no significant associations were observed between the traditional Chinese and high-salt patterns and the risk of depressive symptoms, even after adjusting for potential confounders. The findings indicate that the Western pattern is associated with an increased risk, and the grains-vegetables pattern is associated with a reduced risk of depressive symptoms.

Empirically Derived Dietary Patterns and Hypertension Likelihood: A Meta-Analysis.

BACKGROUND/AIMS: Dietary patterns, which represent whole-diet and a complex integration of food and nutrient, have been reported to play an important role in the development of hypertension. However, the results have yielded conflicting findings. Herein, we performed this meta-analysis to evaluate the associations between different dietary patterns and the likelihood of hypertension. METHODS: MEDLINE and EBSCO were searched to identify relevant articles published until the end of March 2016. A random-effects model was used to account for possible heterogeneity between studies.A total of twenty-seven studies met the inclusion criteria and were included in this meta-analysis. RESULTS: There was evidence of a decreased likelihood for hypertension in the highest compared with the lowest categories of healthy pattern (odds ratio (OR)=0.81; 95% confidence interval(CI): 0.67-0.97; P=0.02). An increased likelihood of hypertension was shown for the highest compared with the lowest category of heavy drinking pattern (OR=1.62; 95% CI: 1.16-2.26; P=0.004), whereas no statistically significant association with western-style and light-moderate drinking pattern were observed(OR=1.04, 95% CI: 0.83-1.31; OR=1.20, 95% CI:0.94- 1.53; P>0.05). CONCLUSIONS: Our results of this systematic review and meta-analysis suggest that dietary pattern may be associated with the likelihood of hypertension.

Elevated Levels of Protein Disulfide Isomerase and Binding Immunoglobulin Protein Implicated in Spinal Cord Injury Paraplegia Patients with Pressure Ulcers.

AIMS: To explore the associations between two endoplasmic reticulum (ER) stress proteins, protein disulfide isomerase (PDI), binding immunoglobulin protein (BIP), and the development and progression of pressure ulcers (PUs) in spinal cord injury (SCI) paraplegia patients. METHODS: ELISA kits were used to measure the levels of serum PDI and BIP in 67 SCI paraplegia patients with PUs and 61 SCI paraplegia patients without PUs. The associations between PDI and BIP, PU formation, PU staging, and pressure ulcer scale for healing (PUSH) score were analyzed. RESULTS: The patients in the PU group had higher levels of PDI and BIP than those in the non-PU group (both p < 0.05). Furthermore, the levels of PDI were positively correlated with those of BIP (r = 0.707, p < 0.0001). There were significant differences in the PDI and BIP levels among the different stages of PU (all p < 0.05). As the PU stages progressed, the levels of PDI and BIP first increased, then decreased, and finally peaked at stage III of the PUs. The PUSH scores significantly declined 7 days after debridement for the PU stage II (p < 0.01) but showed no significant difference between stages III and IV at 7 days after debridement (p > 0.05). The PUSH scores also decreased at 28 days after debridement for stages II, III, and IV (all p < 0.01). Higher PUSH scores indicated a longer time of debridement accompanied by a longer wound surface healing time (p < 0.05). CONCLUSION: ER stress proteins may be involved in the process of PU formation and healing; moreover, the levels of PDI and BIP were also associated with the severity of the PUs. Finally, we found that the PUSH scores can be used as a reference to evaluate PU severity and healing.