ABSTRACT
Thunberg fritillary bulb (the dry bulbs of Fritillaria thunbergii Miq.), a traditional Chinese Medicine, is widely applied as an expectorant and antitussive. In this investigation, the primary metabolites of bulbs, flowers, leaves, and stems of F. thunbergii were analyzed by gas chromatography-mass spectrometry. Principal component analysis, partial least squares-discriminate analysis, orthogonal projection to latent structures-discriminate analysis, and heat map analysis showed that there were dissimilar metabolites, and a negative correlation between amino acids and saccharides in different analytes. Furthermore, carbodiimide, tryptophan, glucose-6-phosphate, xylose, 2-piperidinecarboxylic acid, monoamidomalonic acid, phenylalanine, and histidine were found to play an important role in the plant metabolism net of F. thunbergii.
Subject(s)
Fritillaria/chemistry , Gas Chromatography-Mass Spectrometry , Metabolome , Metabolomics , Computational Biology/methods , Data Analysis , Fritillaria/metabolism , Metabolic Networks and Pathways , Metabolomics/methodsABSTRACT
A sensitive reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for rapid visual detection of Murray valley encephalitis virus (MVEV) infection. The reaction was performed in one step in a single tube at 63 °C for 60 min with the addition of the hydroxynaphthol blue (HNB) dye prior to amplification. The detection limit of the RT-LAMP assay was 100 copies per reaction based on 10-fold dilutions of in vitro transcribed RNA derived from a synthetic MVEV DNA template. No cross-reaction was observed with other encephalitis-associated viruses. The assay was further evaluated using spiked cerebrospinal fluid sample with pseudotype virus containing the NS5 gene of MVEV.
Subject(s)
Encephalitis Virus, Murray Valley/isolation & purification , Transcription, Genetic , Base Sequence , DNA Primers , Encephalitis Virus, Murray Valley/genetics , Limit of DetectionABSTRACT
OBJECTIVE: To develop a headspace gas chromatography method for the determination of residual organic solvents in Panax notoginseng extracts. METHODS: The samples were injected into HP-INNOWAX capillary column by headspace sampler and analyzed with FID detector using standard addition method. RESULTS: There was a good linearity in the experimental concentration (r=0.9932-0.9999). The rate of recovery was in the rang of 81.74%-111.2%. The numbers of theoretical plates were more than 15000 and the resolutions between the adjacent peaks were more than 2. The RSD of precision and accuracy were both less than 10 %. CONCLUSION: The method is simple,accurate and sensitive with good reproducibility, which can be used for the determination of the residual organic solvents in Panax notoginseng extracts.
