Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 1.032
Filter
2.
Can J Cardiol ; 2024 Jul 04.
Article in English | MEDLINE | ID: mdl-38971496

ABSTRACT

Comparison of [68Ga]Ga-FAPI-04 PET/MR imaging in patients with stable ascending aortic aneurysm (AA) and rapidly expanding ascending AA. Abnormal uptake of [68Ga]Ga-FAPI-04 was found only in the wall of the progressive ascending AA. The pathology of the aortic wall segments demonstrated greater [68Ga]Ga-FAPI-04 uptake showed greater fibroblast activation protein expression, more elastic fiber degradation and collagen deposition than did the adjacent normal aorta.

3.
Nutrients ; 16(13)2024 Jun 29.
Article in English | MEDLINE | ID: mdl-38999839

ABSTRACT

OBJECTIVE: This study describes the association between taste preference for salt and actual salt intake, thus guiding and refining personal and public health campaigns designed to lower salt intake in China. METHODS: A cross-sectional survey of 1489 residents aged 18 to 69 years was conducted in 2017 in China. A multistage random sampling strategy was used, and a combination of questionnaires and physical and laboratory measurements were conducted to collect baseline characteristics and knowledge, attitudes, and behavior (KAB) related to salt. A 24 h urine collection was obtained for sodium and potassium excretion analysis. Participants were divided into two groups, light taste preference and salty taste preference, according to their answer to the question "Compared to others, how do you think your taste preference is for salt?". RESULTS: The mean age of the 1489 participants was 46.26 years, 48.9% were males, over 1/3 (35.7%) were identified as hypertensive, and 317 (21.3%) self-reported a salty taste preference. The mean of 24 h urinary sodium excretion was 167.32 mmol/24 h, corresponding to 9.79 g salt/d intake, and the sodium-to-potassium ratio (Na/K) was 4.90. The 24 h urinary sodium excretion of salty taste preference (177.06 mmol/24 h) was significantly higher than that of light taste preference (164.69 mmol/24 h). The multiple logistic regression analysis showed that the salty taste preference group had significantly higher 24 h urinary sodium (ORa(95%CI) = 1.004(1.002-1.006)), diastolic blood pressure (DBP), proportion of greasy food preference, and drinking levels, but lower potassium excretion, response levels to most KAB questions, and regular physical activity compared to the light taste preference group. CONCLUSION: Self-reported taste preference for salt predicted actual salt intake, which was verified by 24 h urinary sodium monitoring. Taste preference for salt could be used as a proxy for intake in terms of targeted salt intake, nutrition, and health education.


Subject(s)
Food Preferences , Sodium Chloride, Dietary , Taste , Humans , Middle Aged , Male , Female , Adult , Sodium Chloride, Dietary/administration & dosage , Sodium Chloride, Dietary/urine , China , Aged , Cross-Sectional Studies , Young Adult , Adolescent , Health Knowledge, Attitudes, Practice , Asian People , Surveys and Questionnaires , East Asian People
4.
Angew Chem Int Ed Engl ; : e202410118, 2024 Jul 12.
Article in English | MEDLINE | ID: mdl-38997791

ABSTRACT

Molecular phosphorescence in the second near-infrared window (NIR-II, 1000-1700 nm) holds promise for deep-tissue optical imaging with high contrast by overcoming background fluorescence interference. However, achieving bright and stable NIR-II molecular phosphorescence suitable for biological applications remains a formidable challenge. Herein, we report a new series of symmetric isocyanorhodium(I) complexes that could form oligomers and exhibit bright, long-lived (7-8 µs) phosphorescence in aqueous solution via metallophilic interaction. Ligand substituents with enhanced dispersion attraction and electron-donating properties were explored to extend excitation/emission wavelengths and enhanced stability. Further binding the oligomers with fetal bovine serum (FBS) resulted in NIR-II molecular phosphorescence with high quantum yields (up to 3.93%) and long-term stability in biological environments, enabling in vivo tracking of single-macrophage dynamics and high-contrast time-resolved imaging. These results pave the way for the development of highly-efficient NIR-II molecular phosphorescence for biomedical applications.

5.
Ann Vasc Surg ; 2024 Jul 16.
Article in English | MEDLINE | ID: mdl-39025220

ABSTRACT

OBJECTIVE: This study aims to introduce the clinical application value of popliteal vein puncture in the supine position under ultrasound guidance and compare this method with popliteal vein puncture in the prone position. METHODS: Endovascular operations for nonthrombotic iliac vein lesions (NIVLs) patients using popliteal vein access were performed during the period from July 2019 to August 2022 at the Zhongshan Hospital (Xiamen), Fudan University and Shanghai Xuhui District Central Hospital. Patients were randomly divided into supine position group and prone position group. All of the patients were punctured under ultrasound guidance. The procedure duration time for popliteal vein puncture, visual analogue score (VAS) scores and postoperative complications were recorded and compared between the two groups. RESULTS: Totally 120 patients were included in this study, in which 60 patients were enrolled in the supine position group, and 60 patients were enrolled in the prone position group. The median procedure time from puncture to iliofemoral venography was 5.97 min (interquartile range 5.78 min -6.03 min) and 28.76min (interquartile range 26.84 min -29.83 min ; p<0.01)in the supine position and prone position group, respectively. The median time from puncture to access sheath insertion was 5.05 min (interquartile range 4.88 min -5.13 min ) and 5.03 min (interquartile range 4.93 min-5.12 min; p =0.607)in the supine position and prone position group, respectively. The median VAS value was 3 (interquartile range 2-3 ) and 8 (interquartile range 7-9 , p<0.01)in the supine position and prone position group, respectively. In the supine position group, 1 case of arterial branch injury was observed after operation, and was successfully managed by ultrasound-guided compression. CONCLUSIONS: Popliteal vein puncture in the supine position under ultrasound guidance is safe and significantly reduces the overall operation time without changing position, and relieves the discomfort of patients.

6.
Virol Sin ; 2024 Jul 16.
Article in English | MEDLINE | ID: mdl-39025463

ABSTRACT

Myocarditis is an inflammatory disease of the cardiac muscle and one of the primary causes of dilated cardiomyopathy. Group B coxsackievirus (CVB) is one of the leading causative pathogens of viral myocarditis, which primarily affects children and young adults. Due to the lack of vaccines, the development of antiviral medicines is crucial to controlling CVB infection and the progression of myocarditis. In this study, we investigated the antiviral effect of baicalein, a flavonoid extracted from Scutellaria baicaleinsis. Our results demonstrated that baicalein treatment significantly reduced cytopathic effect and increased cell viability in CVB3-infected cells. In addition, significant reductions in viral protein 3D, viral RNA, and viral particles were observed in CVB3-infected cells treated with baicalein. We found that baicalein exerted its inhibitory effect in the early stages of CVB3 infection. Baicalein also suppressed viral replication in the myocardium and effectively alleviated myocarditis induced by CVB3 infection. Our study revealed that baicalein exerts its antiviral effect by inhibiting the activity of caspase-1 and viral protease 2A. Taken together, our findings demonstrate that baicalein has antiviral activity against CVB3 infection and may serve as a potential therapeutic option for the myocarditis caused by enterovirus infection.

7.
J Colloid Interface Sci ; 672: 392-400, 2024 Oct 15.
Article in English | MEDLINE | ID: mdl-38848623

ABSTRACT

The rational design and synthesis of carbon nanocages with highly complex porous structures are continuously facing challenges in the development of high-performance supercapacitors (SCs). The electrochemical performance characteristics of electrodes rely on their compositions and fabrication methods. Here, we propose a universal and efficient approach for the in-situ synthesis of zeolitic imidazolate framework-8 (ZIF-8) on porous carbonized wood, where the selective utilization of hexacarbonyl molybdenum protects the structural integrity of the ZIF-8 precursor, preventing collapse during thermal treatment. The subsequent pyrolysis process leads to the formation of small-sized molybdenum carbide (MoC) which are embedded in carbon nanocages (CN). The composite electrode consists of MoC/CN embedded in a porous carbonized wood (PCW), and it shows area-specific capacity of 9.7F cm-2 and 9.4 F cm-2 at 5 mA cm-2 and 30 mA cm-2, respectively. Subsequently, the symmetric supercapacitor, with two MoC/CN@PCW electrodes exhibits a areal specific capacitance of 2.7 F cm-2 at 5 mA cm-2. Moreover, this supercapacitor maintains an capacitance retention rate of 98.5 % after 12,000 discharge cycles. The supercapacitor exhibits a power density of 6.5 mW cm-2, resulting in an energy density of 0.864 mWh cm-2. Therefore, the utilization of wood-based electrodes holds promise for energy storage systems.

8.
Adv Mater ; : e2405761, 2024 Jun 24.
Article in English | MEDLINE | ID: mdl-38923441

ABSTRACT

Abdominal aortic aneurysm (AAA) is a highly lethal cardiovascular disease that currently lacks effective pharmacological treatment given the complex pathophysiology of the disease. Here, single-cell RNA-sequencing data from patients with AAA and a mouse model are analyzed, which reveals pivotal pathological changes, including the M1-like polarization of macrophages and the loss of contractile function in smooth muscle cells (SMCs). Both cell types express the integrin αvß3, allowing for their dual targeting with a single rationally designed molecule. To this end, a biocompatible nanodrug, which is termed EVMS@R-HNC, that consists of the multifunctional drug everolimus (EVMS) encapsulated by the hepatitis B virus core protein modifies to contain the RGD sequence to specifically bind to integrin αvß3 is designed. Both in vitro and in vivo results show that EVMS@R-HNC can target macrophages as well as SMCs. Upon binding of the nanodrug, the EVMS is released intracellularly where it exhibits multiple functions, including inhibiting M1 macrophage polarization, thereby suppressing the self-propagating inflammatory cascade and immune microenvironment imbalance, while preserving the normal contractile function of SMCs. Collectively, these results suggest that EVMS@R-HNC presents a highly promising therapeutic approach for the management of AAA.

9.
Article in English | MEDLINE | ID: mdl-38940798

ABSTRACT

Background: Hepatocellular carcinoma (HCC) presents a challenging global health concern due to its high incidence and limited treatment efficacy. Understanding the molecular pathways driving HCC development is crucial for advancing therapeutic strategies and improving patient outcomes. Objective: This study aims to assess the impact of Salinomycin on the Wnt/ß-catenin signaling pathway in hepatocellular carcinoma, exploring its role in tumor migration and angiogenesis. Additionally, to explore the therapeutic potential of targeting this pathway for improving HCC treatment outcomes. Methods: This study employed an in vitro experimental design to investigate the role of the Wnt/ß-catenin signaling pathway in HCC progression. HepG2 cells were cultured in RPMI 1640 medium supplemented with 10% serum, penicillin (100 U/ml), and streptomycin (100 µg/ml). The cells were divided into experimental and control groups for comparative analysis. Salinomycin was administered to inhibit Wnt/ß-catenin pathway activation. The primary outcome measures included the evaluation of cell migration and tumor angiogenesis using cell migration assays and vascular endothelial growth factor (VEGF) expression analysis, respectively. Statistical analysis was performed using the two-tailed Student's t test, with significance set at P < .05. Results: Salinomycin treatment led to a dose-dependent decrease in HCC cell proliferation, with optical density values decreasing as the concentration of Salinomycin increased. Cell migration assays showed inhibited migration in cells treated with Salinomycin compared to controls. Western blot analysis revealed decreased levels of ß-catenin and increased levels of DVL in Salinomycin-treated cells, indicating inhibition of the Wnt/ß-catenin pathway. Furthermore, VEGF expression decreased after Salinomycin treatment, implicating the pathway in tumor angiogenesis. Statistical analysis, including Student's t-test, confirmed significant differences between control and experimental groups (P < .05). Conclusion: The Wnt/ß-catenin signaling pathway plays a significant role in the migration and angiogenesis of HCC when treated with Salinomycin.

10.
Cancer Immunol Immunother ; 73(8): 137, 2024 Jun 04.
Article in English | MEDLINE | ID: mdl-38833034

ABSTRACT

Tumor-infiltrating lymphocyte (TIL) deficiency is the most conspicuous obstacle to limit the cancer immunotherapy. Immune checkpoint inhibitors (ICIs), such as anti-PD-1 antibody, have achieved great success in clinical practice. However, due to the limitation of response rates of ICIs, some patients fail to benefit from monotherapy. Thus, novel combination therapy that could improve the response rates emerges as new strategies for cancer treatment. Here, we reported that the natural product rocaglamide (RocA) increased tumor-infiltrating T cells and promoted Th17 differentiation of CD4+ TILs. Despite RocA monotherapy upregulated PD-1 expression of TILs, which was considered as the consequence of T cell activation, combining RocA with anti-PD-1 antibody significantly downregulated the expression of PD-1 and promoted proliferation of TILs. Taken together, these findings demonstrated that RocA could fuel the T cell anti-tumor immunity and revealed the remarkable potential of RocA as a therapeutic candidate when combining with the ICIs.


Subject(s)
Benzofurans , Cell Differentiation , Immune Checkpoint Inhibitors , Lymphocytes, Tumor-Infiltrating , Programmed Cell Death 1 Receptor , Immune Checkpoint Inhibitors/pharmacology , Immune Checkpoint Inhibitors/therapeutic use , Lymphocytes, Tumor-Infiltrating/immunology , Lymphocytes, Tumor-Infiltrating/drug effects , Lymphocytes, Tumor-Infiltrating/metabolism , Mice , Animals , Benzofurans/pharmacology , Benzofurans/therapeutic use , Programmed Cell Death 1 Receptor/antagonists & inhibitors , Humans , Cell Differentiation/drug effects , Neoplasms/immunology , Neoplasms/drug therapy , Neoplasms/pathology , Drug Resistance, Neoplasm/drug effects , Mice, Inbred C57BL , Female , T-Lymphocytes/immunology , T-Lymphocytes/drug effects , T-Lymphocytes/metabolism , Cell Line, Tumor
11.
J Immunother Cancer ; 12(6)2024 Jun 26.
Article in English | MEDLINE | ID: mdl-38926151

ABSTRACT

BACKGROUND: Lung metastasis is the primary cause of breast cancer-related mortality. Neutrophil extracellular traps (NETs) are involved in the progression of breast cancer. However, the mechanism of NET formation is not fully understood. This study posits that tumor cell-released autophagosomes (TRAPs) play a crucial role in this process. METHODS: TRAPs were isolated from breast cancer cell lines to analyze their impact on NET formation in both human and mouse neutrophils. The study used both in vitro and in vivo models, including Toll-like receptor 4 (TLR4-/-) mice and engineered breast cancer cell lines. Immunofluorescence, ELISA, Western blotting, RNA sequencing, and flow cytometry were employed to dissect the signaling pathways leading to NET production and to explore their immunosuppressive effects, particularly focusing on the impact of NETs on T-cell function. The therapeutic potential of targeting TRAP-induced NETs and their immunosuppressive functions was evaluated using DNase I and αPD-L1 antibodies. Clinical relevance was assessed by correlating circulating levels of TRAPs and NETs with lung metastasis in patients with breast cancer. RESULTS: This study showed that TRAPs induced the formation of NETs in both human and mouse neutrophils by using the high mobility group box 1 and activating the TLR4-Myd88-ERK/p38 signaling axis. More importantly, PD-L1 carried by TRAP-induced NETs inhibited T-cell function in vitro and in vivo, thereby contributing to the formation of lung premetastatic niche (PMN) immunosuppression. In contrast, Becn1 KD-4T1 breast tumors with decreased circulating TRAPs in vivo reduced the formation of NETs, which in turn attenuated the immunosuppressive effects in PMN and resulted in a reduction of breast cancer pulmonary metastasis in murine models. Moreover, treatment with αPD-L1 in combination with DNase I that degraded NETs restored T-cell function and significantly reduced tumor metastasis. TRAP levels in the peripheral blood positively correlated with NET levels and lung metastasis in patients with breast cancer. CONCLUSIONS: Our results demonstrate a novel role of TRAPs in the formation of PD-L1-decorated NETs, which may provide a new strategy for early detection and treatment of pulmonary metastasis in patients with breast cancer.


Subject(s)
Autophagosomes , B7-H1 Antigen , Breast Neoplasms , Extracellular Traps , Lung Neoplasms , Animals , Humans , Mice , Female , Breast Neoplasms/pathology , Breast Neoplasms/immunology , Breast Neoplasms/metabolism , Lung Neoplasms/secondary , Extracellular Traps/metabolism , B7-H1 Antigen/metabolism , Autophagosomes/metabolism , T-Lymphocytes/immunology , T-Lymphocytes/metabolism , Cell Line, Tumor
13.
J Am Chem Soc ; 146(25): 17201-17210, 2024 Jun 26.
Article in English | MEDLINE | ID: mdl-38874405

ABSTRACT

As one of the most lethal cardiovascular diseases, aortic dissection (AD) is initiated by overexpression of reactive oxygen species (ROS) in the aorta that damages the vascular structure and finally leads to massive hemorrhage and sudden death. Current drugs used in clinics for AD treatment fail to efficiently scavenge ROS to a large extent, presenting undesirable therapeutic effect. In this work, a nanocatalytic antioxidation concept has been proposed to elevate the therapeutic efficacy of AD by constructing a cobalt nanocatalyst with a biomimetic structure that can scavenge pathological ROS in an efficient and sustainable manner. Theoretical calculations demonstrate that the antioxidation reaction is catalyzed by the redox transition between hydroxocobalt(III) and oxo-hydroxocobalt(V) accompanied by inner-sphere proton-coupled two-electron transfer, forming a nonassociated activation catalytic cycle. The efficient antioxidation action of the biomimetic nanocatalyst in the AD region effectively alleviates oxidative stress, which further modulates the aortic inflammatory microenvironment by promoting phenotype transition of macrophages. Consequently, vascular smooth muscle cells are also protected from inflammation in the meantime, suppressing AD progression. This study provides a nanocatalytic antioxidation approach for the efficient treatment of AD and other cardiovascular diseases.


Subject(s)
Antioxidants , Aortic Dissection , Cobalt , Catalysis , Cobalt/chemistry , Cobalt/pharmacology , Aortic Dissection/drug therapy , Aortic Dissection/pathology , Antioxidants/chemistry , Antioxidants/pharmacology , Animals , Biomimetic Materials/chemistry , Biomimetic Materials/pharmacology , Biomimetic Materials/chemical synthesis , Mice , Reactive Oxygen Species/metabolism , Humans , Oxidative Stress/drug effects , Metal Nanoparticles/chemistry
14.
Sci Total Environ ; 940: 173614, 2024 Aug 25.
Article in English | MEDLINE | ID: mdl-38823708

ABSTRACT

Gully is a prominent indicator of land degradation in agroecosystems, functioning as a crucial pathway connecting upslopes to downstream channels. However, little is known about how gully regulates runoff, sediment, and nutrient loss processes in the catchment during snowmelt. In this study, we monitored these processes in situ at both the gully head (the upslope accumulated catchment of the gully head, CGH) and outlet of two representative and typical gully-dominated catchments (F1 and F2) during snowmelt in Mollisols region of Northeast China. Our results showed that runoff discharge of CGH and outlet exhibited a multi-peak trend during snowmelt, driven by the transition from snow melting to soil thawing. This transition resulted in distinct runoff patterns in both CGH and outlet, with significant differences in their response to air temperature. The total runoff yield of CGH accounted for 57.8 % in F1 and 40.6 % in F2 of the total runoff yield of the outlet. Notably, the peak sediment concentration displayed a marked lag compared to the peak runoff discharge, primarily dominated by the increased sensitivity of gully erosion after the thawing of gully slopes. Gully erosion was the main source of sediment yield in the catchment, contributing 98.2 % in F1 and 96.6 % in F2. Furthermore, nutrient concentrations exhibited a decreasing trend during snowmelt. The comparison of high nutrient concentrations in CGH and relatively low nutrient concentrations in outlet highlighted the gully's role in intercepting and diluting runoff nutrients. Hysteresis analysis confirmed the differential contribution of CGH and gully to nutrient sources. CGH accounting for 50.9 % and 93.3 % of runoff TN and runoff TP loss, while contributing only 8.3 % and 5.8 % to sediment TN and sediment TP loss, respectively. These findings offer valuable insights for effective erosion control and nonpoint source pollution management in gully-dominated agroecosystems during snowmelt.

15.
Surg Endosc ; 2024 Jun 24.
Article in English | MEDLINE | ID: mdl-38914885

ABSTRACT

BACKGROUND: Endoscopic balloon dilation (EBD) is a safe and effective treatment for Crohn's disease (CD)-associated strictures. However, serial EBDs have rarely been reported. This study aimed to evaluate the efficacy and safety of serial EBDs for treating CD-associated duodenal strictures compared with intermittent EBDs. METHODS: Patients with CD-associated duodenal strictures who underwent EBD were recruited. The clinical data, stricture characteristics, number of EBDs, dilation diameter, complications, surgical interventions, and follow-up periods were recorded. Patients were divided into a serial dilation group and an intermittent dilation group to analyze the differences in safety and efficacy. RESULTS: Forty-five patients with duodenal CD-associated strictures underwent a total of 139 dilations. A total of 23 patients in the serial dilation group underwent 72 dilations, for a median of 3 (range 3 ~ 4) dilations per patient, and 22 patients in the intermittent dilation group underwent 67 dilations, for a median of 3 (range 1 ~ 6) dilations per patient. Technical success was achieved in 97.84% (136/139) of the patients. During the follow-up period, three patients in the intermittent dilation group underwent surgery, and the total clinical efficacy was 93.33% (42/45). No difference in safety or short-term efficacy was noted between the two groups, but serial EBDs exhibited significantly greater clinical efficacy between 6 months and 2 years. No significant difference in recurrence-free survival was observed, but the median longest recurrence-free survival and recurrence-free survival after the last EBD in the serial dilation group were 693 days (range 298 ~ 1381) and 815 days (range 502 ~ 1235), respectively, which were significantly longer than the 415 days (range 35 ~ 1493) and 291 days (range 34 ~ 1493) in the intermittent dilation group (p = 0.013 and p = 0.000, respectively). At the last follow-up, the mean diameter of the duodenal lumen was 1.17 ± 0.07 cm in the serial dilation group, which was greater than the 1.11 ± 0.10 cm in the intermittent dilation group (p = 0.018). We also found that the Simple Endoscopic Score for Crohn's Disease was associated with an increased risk of surgical intervention (HR 2.377, 95% CI 1.125-5.020; p = 0.023) and recurrence at 6 months after the last EBD (HR 0.698, 95% CI 0.511-0.953; p = 0.024), as assessed by univariate analysis. CONCLUSIONS: Compared to the intermittent EBDs, serial EBDs for duodenal CD-associated strictures exhibit greater clinical efficacy within two years and could delay stricture recurrence. We suggest that serial EBDs can be a novel option for endoscopic treatment of duodenal CD-associated strictures.

16.
Environ Int ; 189: 108794, 2024 Jul.
Article in English | MEDLINE | ID: mdl-38833876

ABSTRACT

Indoor semivolatile organic compounds (SVOCs) pose a substantial threat to human health. However, identifying the sources of these emissions has been challenging owing to the scarcity of convenient and practical on-site methodologies. Herein, a novel method for source screening was proposed using aluminum silicate sampling strips to adsorb SVOCs from the surface air of indoor materials. The adsorbed SVOC levels indicate the emission intensity of these materials into indoor environments. Additionally, compact sampling strips can be readily fixed to any vertical surface using a static sticker, facilitating the characterization of various materials in practical settings. Laboratory-simulated experiments demonstrated the capability of the proposed method to differentiate between source and non-source materials within a 10-cm distance in the same space. In practical scenarios, the primary emission sources identified via this method exhibited a consistent correlation with the contents of the corresponding materials obtained from the traditional solvent-extraction method. As the adsorbed SVOCs were directly transferred to a GC-MS through thermal desorption instead of the solvent-extraction procedure, the proposed method demonstrated several-fold improvements in analytical sensitivity and efficiency. Using this versatile screening technique, some emerging and important SVOC species were identified within specific indoor materials. Eliminating these sources has been demonstrated as an effective approach to mitigate SVOC pollution. Overall, the proposed method offers a powerful tool for managing indoor pollutants and safeguarding human health.


Subject(s)
Air Pollution, Indoor , Environmental Monitoring , Volatile Organic Compounds , Air Pollution, Indoor/analysis , Volatile Organic Compounds/analysis , Environmental Monitoring/methods , Gas Chromatography-Mass Spectrometry/methods , Air Pollutants/analysis , Humans
18.
Article in English | MEDLINE | ID: mdl-38717638

ABSTRACT

BACKGROUND: Cardiac hypertrophy is the common pathological process of multiple cardiovascular diseases. However, the molecular mechanisms of cardiac hypertrophy are unclear. Long non-coding RNA (lncRNA), a newly discovered type of transcript that has been demonstrated to function as crucial regulators in the development of cardiovascular diseases. This study revealed a novel regulatory pathway of lncRNA in cardiac hypertrophy. METHODS: The cardiac hypertrophy models were established by transverse aortic constriction (TAC) in mice and angiotensin II (Ang II) in HL-1 cardiomyocytes. Adeno-associated virus 9 (AAV9) in vivo and lncRNA Gm15834 and shRNA plasmids in vitro were used to overexpress and knock down lncRNA Gm15834. The myocardial tissue structure, cardiomyocyte area, cardiac function, protein expressions, and binding of lncRNA Gm15834 and Src-associated substrate during mitosis of 68 KDa (Sam68) were detected by hematoxylin and eosin (HE) staining, immunofluorescence staining, echocardiography, western blot and RNA immunoprecipitation (RIP), respectively. RESULTS: In cardiac hypertrophy models, inhibiting lncRNA Gm15834 could decrease Sam68 expression and nuclear factor kappa-B (NF-κB) mediated inflammatory activities in vivo and in vitro, but overexpressing lncRNA Gm15834 showed the opposite results. RIP experiments validated the binding activities between lncRNA Gm15834 and Sam68. Overexpression of Sam68 could counteract the anti-hypertrophy effects of lncRNA Gm15834 knockdown. Meanwhile, in vivo inhibition of lncRNA Gm15834 could inhibit Sam68 expression, reduce NF-κB mediated inflammatory activity and attenuate cardiac hypertrophy. CONCLUSION: Our study revealed a novel regulatory axis of cardiac hypertrophy, which comprised lncRNA Gm15834/Sam68/NF-κB/inflammation, shedding a new light for identifying therapy target of cardiac hypertrophy in clinic.

19.
RSC Adv ; 14(21): 15071-15084, 2024 May 02.
Article in English | MEDLINE | ID: mdl-38720976

ABSTRACT

The alkane cracking mechanism has been a subject of intense scrutiny, with carbonium and free radical mechanisms being two well-established pathways which correlate to solid acid catalysis and thermal cracking, respectively. However, despite an understanding of these two mechanisms, certain intricacies remain unexplored, especially when it comes to alternative reaction routes over solid base materials. This gap in the knowledge hinders optimization of the desired product selectivity of alkane cracking processes. In this work, solid superbases were first prepared by impregnation of NaNO3 on MgO. The Na/MgO catalysts were characterized by XRD, BET, XPS and CO2-TPD techniques. To investigate the role of solid base materials, propane cracking was conducted over MgO and Na/MgO. SiO2 was chosen as a representative of thermal cracking. Na/MgO showed better selectivity for light olefins than MgO or SiO2. Ethylene and light olefin selectivity could reach about 65.8% and 91.7%, respectively. Meanwhile, in terms of Na/MgO, the ratio of ethylene selectivity and propylene selectivity is greater than 2, exhibiting the advantage of selectivity for ethylene, which is obviously different from MgO and SiO2. Propane cracking over Na/MgO with different loading amounts of NaNO3 was investigated further. The conversion rates of the samples presented a "volcano curve" with increasing Na content. Furthermore, DFT calculation showed that the base-catalyzed process of the propane cracking reaction follows a carbanion mechanism. The better product distribution and stronger surface base sites can be ascribed to charge transfer arising from the loading of NaNO3.

20.
Int J Ophthalmol ; 17(3): 435-443, 2024.
Article in English | MEDLINE | ID: mdl-38721521

ABSTRACT

AIM: To investigate the underlying mechanism of dry environment (autumn dryness) affecting the lacrimal glands in rats. METHODS: Twenty Sprague-Dawley rats were randomly divided into two groups. The rats were fed in specific pathogen free environment as the control group (n=10), and the rats fed in dry environment as the dryness group (n=10). After 24d, lacrimal glands were collected from the rats. The tissues morphology was observed by hematoxylin-eosin (HE) staining. Tandem mass tags (TMT) quantitative proteomics analysis technology was used to screen the differential expressed proteins of lacrimal glands between the two groups, then bioinformatics analysis was performed. Further, the immunohistochemical (IHC) method was used to verify the target proteins. RESULTS: In dryness group, the lacrimal glands lobule atrophied, the glandular cavities enlarged, the sparse nuclear distribution and scattered inflammatory infiltration between the acinus were observed. The proteomics exhibited that a total of 195 up-regulated and 236 down-regulated differential expressed proteins screened from the lacrimal glands of rats. It was indicated that the biological processes (BP) of differential expressed proteins mainly included cell processes and single BP. The cellular compositions of differential expressed proteins mainly located in cells, organelles. The molecular functions of differential expressed proteins mainly included binding, catalytic activity. Moreover, the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the differential expressed proteins mainly involved lysosome, complement and coagulation cascade, and ribosome pathway. The IHC result verified that the up-regulated expression proteins of Protein S100A9 (S100A9), Annexin A1 (Anxa1), and Clusterin (Clu) in lacrimal glands of rats in dryness group were higher than control group. CONCLUSION: The up-regulated expression proteins of S100A9, Anxa1, and Clu may be the potential mechanisms of dry eye symptoms caused by dry environment. This study provides clues of dry environments causing eye-related diseases for further studies.

SELECTION OF CITATIONS
SEARCH DETAIL
...