ABSTRACT
The iron response element (IRE) is a highly conserved RNA stem loop structure. It is the binding site of iron regulatory protein (IRP). IRP binding to IRE is regulated by cellular iron. When cells are derived of iron, IRP binds IRE. If IRE is located at 5'UTR, IRP binding will inhibit translation initiation, else if IRE is at 3'UTR, IRP binding will stabilize mRNA and prevent it from degradation. So far all known IREs have C at the 1 position and G at the 5 position of the loop (C1G5 type). In vitro studies suggest that the U1A5 type IRE, which has U and A at the 1 and 5 loop position respectively, binds well to IRP. However, U1A5 type's in vivo existence is still elusive. IRE-IRP binding is involved in the regulation of iron metabolism, oxidative stress and possibly aging. Here we use an improved computation method performing a comprehensive search of IRE in human and mouse genes. We try to catalog potential human and mouse IRE containing genes, at the same time identify potential U1A5 IREs.
Subject(s)
3' Untranslated Regions/genetics , 5' Untranslated Regions/genetics , Iron/pharmacology , Response Elements/genetics , Software , 3' Untranslated Regions/chemistry , 5' Untranslated Regions/chemistry , Animals , Base Sequence , Binding Sites/genetics , Gene Expression Regulation/drug effects , Humans , Iron/metabolism , Iron-Binding Proteins/metabolism , Mice , Nucleic Acid Conformation , Response Elements/drug effectsABSTRACT
A new lactone compound named cannabifolactone A was isolated from the water extract of the aerial parts of Senecio cannabifolius Less. Its structure was elucidated mainly by 1D- and 2D-NMR techniques.
