Potential risks of accumulated microplastics in shells and soft tissues of cultured hard clams (Meretrix taiwanica) and associated metals.

Microplastics (MPs) pose risks to both aquatic ecosystems and human health. This study investigated MPs in the shells and soft tissues of hard clams (Meretrix taiwanica) cultured in the inland waters of Taiwan. This study further developed two novel risk indices for assessing the potential ecological and health consequences of MPs. Moreover, the metal concentrations in the clam's soft tissues and the associated consumption health risks were investigated. Clamshells contained significant amounts of MPs with an average abundance of 16.6 ± 6.9 MPs/ind., which was higher than in the soft tissues (2.7 ± 1.7 MPs/ind.). The distribution and sizes of MPs in shells and soft tissues were similar, primarily small-sized (<2 mm, >99 %), blue (>65 %), and fibrous (>99 %). Dominant MP polymer types included rayon (83.5 %), polyethylene terephthalate (11.8 %), and polyacrylonitrile (3.6 %). The proposed MP potential ecological risk index indicates a higher potential ecological MP risk in soft tissues (302-423) than in shells (270-278) of the clams. The MP potential hazard risk index showed that the risk of exposure to MP through shellfish consumption decreased with age. The total hazard index (THI) value suggested negligible health hazards from metal exposure through shellfish consumption. Moreover, there was no significant correlation between MPs and metal concentrations in soft tissues, suggesting that metals bound to MPs contribute minimally to the total accumulated metals in clam's soft tissues. This study confirms the presence of MPs in clam shells and provides a novel tool to assess the potential ecological and health risks associated with MPs in shellfish.

Controlling effects of terrestrial organic matter on metal contamination and toxicity risks in port sediments.

The contents of metals, total carbon, total nitrogen (TN), total organic carbon (TOC), and stable isotope composition (δ13Corg and δ15N) of sediment organic matter (SOM) were investigated to explore the sources and spatial distribution of metals and SOM in the surface sediments (Kaohsiung Port, Taiwan). Results showed that TOC and metals in estuarine sediments are high, gradually decreasing toward the port entrances. The δ13Corg, δ15N, and TOC/TN ratios indicate that SOM comes mainly from terrestrial sources. This study proposes a befitting model between metal pollution and toxicity risk index and SOM sources in port sediments by combining stable isotope composition, correlation matrix, and multiple linear regression analysis. The model indicates that the degree of metal pollution and toxicity risk in sediments are mainly affected by TOCterr content and SOM source. The results help to understand the influence of organic matter sources in port sediments on metal concentration distribution.

Phthalate esters and nonylphenol concentrations correspond with microplastic distribution in anthropogenically polluted river sediments.

This paper presents the phthalate esters (PAEs), nonylphenol (NPs), and microplastics (MPs) in river sediments. Results showed that sediments near residential areas were mainly composed of fine particles, potentially influencing the adsorption of PAEs and NPs in the area. The concentrations of Σ10 PAEs in the sediments ranged between 2448 and 63,457 µg/kg dw, dominated by DEHP and DnOP. Microplastics were detected in all samples, with higher abundances found in sediments near residential areas dominated by polypropylene. Toxicological risk assessment indicated potential risks to sensitive aquatic organisms exposed to the sediments. Correlations between MPs, PAEs, and NPs suggest that MPs may serve as possible sources of PAEs in the sediments. Principal component analysis explained 95.4 % of the pollutant variability in the sediments. Overall, this study emphasizes the significance of monitoring and understanding the presence and interactions of PAEs and MPs in river sediments to assess their potential impacts on aquatic ecosystems.

Polycyclic aromatic hydrocarbons in 55-120 µm phytoplankton.

A baseline study was undertaken on polycyclic aromatic hydrocarbons (PAHs) in phytoplankton. Plankton samples from six stations (duplicates) in Kaohsiung Harbor (KH), Taiwan along with a phytoplankton control sample afar from the harbor, were collected. We applied size-fractionation to isolate phytoplankton (55-120 µm), followed by sedimentation and centrifugation to remove abiogenic particulates. The phytoplankton was freeze-dried, extracted with acetone: n-hexane (1:1, v/v), and analyzed using GC-MS. ΣPAHs in phytoplankton ranged between 5204 and 28,903 ng/g dry weight (mean: 12,150 ng/g). The ΣPAHs in KH were >7 times than the control site (C1: 3972 ng/g). Cluster analysis showed spatial gradients (northern < southern KH). Accumulated PAHs in phytoplankton were from petrogenic (fishing ports and ships) and pyrogenic (river outflows), dominated by lower-ring PAHs, likely due to their higher bioavailability in the dissolved phase. We present a practical phytoplankton isolation technique for more accurate phytoplankton PAH concentrations with insights into their distribution and sources.

Microplastics in coastal farmed oyster (Crassostrea angulata) shells: Abundance, characteristics, and diversity.

One of the most concerning emerging pollutants is microplastics (MPs), which can infiltrate soft tissues of organisms by ingestion, adhesion, and fusing and may even become embedded in biominerals. However, very little evidence is available about MPs in biominerals found in the wild. This study detected the abundance and characteristics of MPs in the shells of farmed oysters (Crassostrea angulata) off the coast of Taiwan and discussed the distribution, accumulation, and diversity in the oyster shells. The results showed that MPs were ubiquitous in oyster shells, with an average abundance of 0.70 ± 0.40 MPs/g. MPs abundance was significantly (p < 0.01) higher in small oyster shells (shell length < 6.5 cm, weight 5-10 g) and inorganic (CaCO3) fraction (HCl digestion) than in large oyster shells (>6.5 cm, 10-25 g) and an organic fraction (H2O2 digestion), respectively. However, there was no significant difference in MPs abundance between the top and bottom shells (p > 0.05). MPs with a size <2 mm accounted for 78.5 %, fibrous MPs for 93.7 %, and rayon for 89.5 %. The MPs diversity integrated index (MPDII) in oyster shells was low (0.27), and the small and fibrous MPs seemed more easily embedded in biominerals. The findings confirm the presence of MPs in oyster shells in coastal environments. In addition, oyster shells may contain higher amounts of MPs than soft tissues 4-5 times, which needs to be confirmed. Further revealing the distribution and accumulation of MPs in water/terrestrial biominerals will help to understand the fate of MPs in the environment.

Occurrence and emission of polycyclic aromatic hydrocarbons from water treatment plant sludge in Taiwan.

The concentrations level and distribution of 16 US EPA polycyclic aromatic hydrocarbon (PAHs) from the water treatment plant (WTP), sewage treatment plant (STP), and industrial water treatment plant (ITP) sludge in Taiwan were determined and then assessed the sources, and potential toxicity (carcinogenic polycyclic aromatic hydrocarbons [CPAHs] and toxic BaP equivalent [TEQ]). Results indicated that the total concentrations of PAHs ranged between 58 and 16,436 µg/kg dw. Among the 17 samples, the 2-4 ring of total PAHs were the predominant compound in three kinds of treatment plant (> 60%). Especially, ITP1 owns 95.8% of 2-4 ring of total PAHs and ITP3 owns 54% of five- and six-ring of total PAHs. The molecular indices and principal component analysis (PCA) were used to determine the source contributions, with the results showing that the contributions of combustion/grass, coal or wood combustion and combustion/ liquid (oil) fossil fuel combustion. A PAH toxicity indicated by TEQ was 2.5-506 µg TEQ/g dw. Although, the results indicated that these were not recommended for land applications, but analyses are beneficial to develop effective management strategies for controlling PAH discharge in treatment plants and establishing strategies for its reuse in managing pollutants.

Non-proportional distribution and bioaccumulation of metals between phytoplankton and zooplankton in coastal waters.

Metal concentrations were concurrently quantified in seawater, phytoplankton, and zooplankton from a heavily impacted coast of southern Taiwan. Combined size and density fractionation were used to accurately quantify metal concentrations in phytoplankton. Cr, Co, Ni, Cu, As, and Pb were analyzed using an inductively coupled plasma mass spectrometer (ICP-MS). As expected, metals significantly increased with an order of seawater < phytoplankton < zooplankton (p < 0.05); but did not differ between estuarine, nearshore, and offshore sites (p > 0.05). Metals were higher along Kaohsiung Harbor and marine outfall diffusion sites, highlighting their major impacts on plankton metal contamination. Notably, phytoplankton (Cr BCF > 100; half of the sites) significantly accumulated more metals contrary to zooplankton (BAF < 10). Metal concentrations and bioaccumulation factors between phytoplankton and zooplankton showed significant negative correlations. This demonstrates a non-proportional distribution and bioaccumulation of metals in phytoplankton and zooplankton-corroborating laboratory findings on zooplankton ability to control metals, irrespective of significantly high bioaccumulation in phytoplankton.

Occurrence and distribution of phthalate esters and microplastics in wastewater treatment plants in Taiwan and their toxicological risks.

Phthalate esters (PAEs) are fat soluble synthetic chemicals, usually regarded as plasticizers for being added in numerous plastic products. Thus, environmental and health hazards of PAEs are associated with increasing plastic pollution. In this study, PAEs from sludge samples collected from water, sewage, and industrial treatment plants (N = 17) were analyzed using Gas Chromatography/Mass Spectrometry. Microplastics (MPs) were also quantified and correlated with PAEs. Results showed the highest average PAE concentrations in sewage treatment plants. The greatest ΣPAEs concentration were found in sewage treatment plant (STP4) with 32,414 µg/kg dw, while the lowest found in water treatment plant (WTP3) with 2062 µg/kg dw. Among different PAEs, di-(2-ethyl hexyl) phthalate (DEHP) contributes the highest. Similarly, DEHP, di-n-octyl phthalate (DnOP) and diisononyl phthalate (DiNP) significantly correlated with the total PAEs indicating their large contribution to sludge contamination. The abundance of microplastics in sludge ranged between 1 and 7 MP/g, highest at ITP6, but not detected in some stations. While microplastics may potentially increase PAEs, there was no significant relationship between ΣPAEs and MP abundance. The estimated human daily intake of DEHP and di-n-butyl phthalate (DnBP) when contaminated sludge be used showed low toxicological risks to exposed adults. This research presents the sludge characteristics, PAEs, and microplastic concentrations in different wastewater treatment plants in Taiwan. PAE contamination was highly contributed by domestic and industrial wastes shown by their significant amounts in STP and ITP. Results further provide evidence for potential sludge recycling (WTP sludge) and application to soil.

Potential sources and toxicity risks of polycyclic aromatic hydrocarbons in surface sediments of commercial ports in Taiwan.

The accumulation of pollutants in the semi-enclosed waters of ports has long been a concern. This study assessed the pollution status, sources, and toxicity risks of 16 polycyclic aromatic hydrocarbons (PAHs) in surface sediments of 7 major ports in Taiwan. Total PAHs concentrations in sediments ranged between 8.4 and 572.5 ng/g dw, with an average of 112.4 ± 136.5 ng/g dw. The 3- and 4-ring PAHs (63 %) were the major constituents of PAHs in the sediments. Diagnostic ratios and positive matrix factorization analyses indicated that PAHs in sediments were mainly contributed by biomass combustion (45.0 %), coal combustion (31.5 %), and vehicle emissions and related fossil fuels (23.5 %). The results of ecological risk assessment showed a low-medium risk of PAHs in the sediments outside the port, whereas most of the sediments within the port presented a medium-high risk. An assessment of the possible human health risks indicated that PAHs were present at acceptable levels.

Assessment of trace metal concentrations in Indian Ocean silky sharks Carcharhinus falciformis and their toxicological concerns.

This study assessed the concentrations of nine trace metals from juvenile C. falciformis caught from Indian Ocean. This study also discussed the metal pollution index (MPI) and bioconcentration factor (BCF) of each element, and their correlations. Further, the potential health risks of consuming shark muscles (THI) were evaluated. Results showed the mean concentrations of 9 elements as follows: Cu (0.36 ± 0.17), Zn (5.19 ± 16.6), Pb (0.12 ± 0.23), Cd (0.17 ± 0.21), Cr (0.57 ± 1.61), Ni (0.086 ± 0.51), As (1.36 ± 0.83), Co (0.000073 ± 0.0074), and V (0.0024 ± 0.0094) mg/kg ww. The BCF values of the elements were higher than 1, with Co and V being the lowest indicating their bioaccumulative behavior. Correlation analysis showed MPI to be highly correlated with Cu, indicating its greater contributions to the total pollution load. Principal components analysis explained 81.0% of the variability in biometric characteristics and metal concentrations. Health risk assessment for consuming shark muscle in Taiwanese male and female adults suggests potential chronic non-carcinogenic health hazards.

Comparative trace metal assessment in phytoplankton using size and density fractionation.

Trace metal assessment in marine phytoplankton is challenging due to complex assemblages and variable amounts of abiogenic suspended particulates. Using aliquots, this study were able to compare trace metal concentrations in plankton samples subjected to size and density fractionation. Elements including Cr, Mn, Fe, Ni, Cu, Zn, As, Sr, Hg, and Pb were analyzed by inductively coupled plasma mass spectrometer (ICP-MS). Trace metals were found to be significantly higher in size fractionated than density fractionated plankton for both small (1.2-50 µm) and large (50-120 µm) fractions. Metals from abiogenic sources (61-88%) also significantly contributed to trace metals detected in 1.2-120 µm suspended particulates collected from Kaohsiung Harbor. Results suggest that size fractionation can potentially overestimate trace metals in phytoplankton. It is therefore recommended combining the two methods by first isolating different size fractions followed by density fractionation to separate phytoplankton from zooplankton, and abiogenic particulates from phytoplankton assemblages, respectively.

Evaluation of polycyclic aromatic hydrocarbons in silky sharks Carcharhinus falciformis collected from Western Indian Ocean and human health risk assessment.

The silky shark (Carcharhinus falciformis) is the most abundant and widely distributed shark species in the marine system of tropical regions. However, it appears that there is limited information on USEPA 16 polycyclic aromatic hydrocarbons (PAHs) concentration in silky shark muscle tissue, which has together with the lack of a health risk assessment of human consumption of silky shark filets. The potential toxicity of PAHs in muscles of silky sharks and cancer and non-cancer risk of consumers were assessed. Results showed that the total PAHs (TPAHs) concentration in the muscle tissue of silky sharks was between 62 and 657 ng/g ww, with an average of 234 ± 221 (mean ± standard deviation) ng/g ww, and can be considered as minimally to moderately contaminated level. Naphthalene (NA), phenanthrene (PH), and fluoranthene (FLU) are the most abundant PAHs in silky sharks muscle tissue, accounting for 38.6%, 11.0%, and 19.0% of TPAHs, respectively. The average values of toxic benzo[a]pyrene (BaP) equivalent (TEQcarc) and toxic dioxin equivalent (TEQfish) of PAHs equal to the potency of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in silky shark muscle are 13.5 ± 16.4 ng BaP/g ww and 34.1 ± 77.4 pg TCDD/g ww, respectively, and they showed a good linear relationship (r = 0.928; p < 0.001). The TEQcarc of silky sharks muscle is higher than USEPA's screening value of PAHs of 3.2 ng BaP/g ww for adults, indicating a potential negative impact on public health. The calculated hazard index (HI) of PAHs is less than 1, suggesting that residents eating silky shark muscle will not expect to cause significant non-cancer risk. However, the incremental lifetime cancer risk (ILCR) of BaP as 10.6-26.3% and 8.2-32.4% in males and females, respectively, may pose a significant cancer risk. In general, children and the elderly have relatively higher risks, as well as males have a higher cancer and non-cancer risk than females.

Assessment of polycyclic aromatic hydrocarbons in seafood collected from coastal aquaculture ponds in Taiwan and human health risk assessment.

The level and distribution of 16 USEPA Polycyclic aromatic hydrocarbon (PAHs) in aquaculture farmed fish (Mugil cephalus and Oreochromis mossambicus) and shellfish (Corbicula fluminea Formosa and Meretrix lusoria) were determined in Taiwan and then assessed cancer and non-cancer risks for those consuming these kinds of seafood. Results indicated that C. fluminea Formosa accumulated the highest average concentration of total PAHs (43.0 ± 11.3 ng/g wet weight) while M. lusoria contained the lowest concentration (20.0 ± 5.8 ng/g) among all species. The low-molecular-weight PAHs were dominant for both fish and shellfish, which consistent with other studies. Notably, parts of high-molecular-weight PAHs were found in shellfish whereas that was little in fish. The calculated hazard quotients (HQ) of all PAHs were smaller than 1 and the incremental lifetime cancer risks (ILCR) for Benzo[a]pyrene were below 1 × 10-5, suggesting that PAHs in the collected seafood could pose a low hazard to residents. Although the results indicated that the studied seafood is safe for human consumption, children and seniors post relatively higher risks, suggesting that it needs to continue monitoring and control the PAHs concentration in seafood and the associated environments.

The effect of heavy rainfall on the exposure risks of sedimentary phthalate esters to aquatic organisms.

Phthalate esters (PAEs) have known widely being used in plastic products leading to being ubiquitous in the environment by easy to release from those products. This study aims to understand the impact of heavy rainfall on the concentration of PAEs in surface sediments of the Salt River in Kaohsiung, Taiwan, and its potential ecological risks on aquatic organisms. The potential ecological risk assessment of sediment PAEs is based on the total risk quotient (TRQ) method. The total concentration of 10 PAEs (∑PAE10) in sediments of the Salt River is 333-13,615 ng/g dw, with an average of 4212 ± 3753 ng/g dw. Before the rainy season, the ∑PAE10 concentration in sediments at the outlets of domestic sewage in upstream was 9768-13,615 ng/g dw, which were relatively higher than other sites (542-3721 ng/g dw). During the rainy season, the ∑PAE10 concentration was 2820-12,041 ng/g dw, which was 1-11 times higher than that determined before the rainy season. After the rainy season, the ∑PAE10 concentration recorded was 530-6652 ng/g dw, which is 1-11 times lower than the value obtained during the rainy season. PAEs in sediments of the Salt River may have low to moderate potential risks to algae, crustaceans, and fish. Bis(2-ethylhexyl) phthalate (DEHP) and diisobutyl phthalate (DiBP) are the main PAE that poses a potential risk to algae and crustaceans, and to fish respectively, whose values of risk quotient accounts for 40-69% of the TRQ value. The distribution of TRQ values for these aquatic organisms show a decreasing trend of PAEs level with respect to the rainy season: during the rainy season > after the rainy season > before the rainy season. Heavy rainfall may cause more serious pollution in sediments and increase the exposure risk of PAEs to aquatic organisms.

Distribution and environmental risk assessment of trace metals in sludge from multiple sources in Taiwan.

This study evaluated the level of the contaminant of the heavy metals in sludge from different sources and the ecological risk criteria associated with it was also analyzed to establish its reuse in agriculture. The sludge samples were collected from the water plant (WTP), wastewater treatment plant (WWTP), and industrial water treatment plant (IPT) in Taiwan. The inductively coupled plasma mass spectrometry was used to measure the trace metals in sludge. The pollution level and ecological risk criteria for heavy metals in sludge were also used to evaluate its reuse in agriculture. The result shows the average concentrations of trace metals in sludge for three groups (WTP, WWTP, and ITP). Significant correlations were found between concentrations of Zn-Ag (p < 0.001). The higher values of Igeo showed in ITP, indicated Hg to be a major pollutant. In Taiwan, the regulations did not establish the reuse of sludge in agriculture. However, the concentration level of trace metals in sludge was particularly lower than the regular levels in most groups, like WTP and WWTP groups. The industrial sludge was not recommended for the use in agriculture. The results of this study can be used for regular monitoring to establish a reference for sludge management and application to agriculture.

Profile and consumption risk assessment of trace elements in megamouth sharks (Megachasma pelagios) captured from the Pacific Ocean to the east of Taiwan.

Focusing on 27 rare filter-feeding megamouth sharks (Megachasma pelagios) captured as a by-catch of drift gillnet fishery in the Pacific Ocean to the east of Taiwan, this study analyzes the concentrations of 24 elements in their muscle, discusses the bioaccumulation of each element and the correlation between different elements, and assesses the potential health risks of consuming megamouth shark muscle. Among the 24 elements, mean concentrations of Ga, Ag, Li, Bi, Hg, Co, and Cd were relatively low ranging from 10-3 to 10-1 mg/kg, those of Pb, Ba, Mn, Ni, As, Cr, B, Sr, Cu, and Zn ranged from 10-1-101 mg/kg, and those of Fe, Ca, Al, K, Mg, Ti, and Na were relatively high ranging from 101 to 103 mg/kg. The toxic element content index was most significantly correlated with the concentration of Cu. Hence, this study recommends that the concentration of Cu could be used as an indicator of metal accumulation in megamouth shark muscle. The log bioconcentration factor (BCF) ranged from less than 0 to 7.85 in shark muscle. For elements with a concentration of less than 100 µg/L in seawater, the log BCF was inversely proportional to their concentration in seawater. According to the correlation analysis, the accumulation of elements in muscle of megamouth sharks is primarily affected by the concentrations of dissolved elements in seawater, except that the accumulation of Hg, As, Cu, Ti, Al, and Fe appears to be mainly affected by feeding behaviors. The assessment of the health risk of consuming megamouth shark muscle showed that its total hazard index was greater than 1. This suggests that the long-term or high-frequency consumption of megamouth shark muscle may cause health hazards due to the accumulation of trace elements, particularly those with a large contribution of health risk, including As, Hg, and Cu.

Detecting phthalate esters in sludge particulates from wastewater treatment plants.

This study proposed a method for analysis of 10 phthalate esters compounds from wastewater treatment plant sludges. The analytical efficiency of GC-MS for of target compounds was verified by a standard mixture of phthalate esters. The response factors related to the respective internal standards from a five-point calibration curve quantified the phthalate esters in individual compounds. Based on the literature compiled by environmental agencies, new generation phthalate compounds have been developed, such as di-iso-nonyl phthalate (DiNP), di-iso-decyl phthalate (DiDP), as alternative to conventional phthalates. The analytical results showed that the total PAEs concentration was in the range from 7.4 to 138.6 mg kg-1 dw in these seven analyzed sludge samples. More, di-iso-nonyl phthalate (DiNP), di-iso-decyl phthalate (DiDP) and bis(2-ethylhexyl) phthalate (DEHP) contributed to over 99% of PAEs in the sludge. The correlation between total PAEs concentration in household and sewage flow treated at seven WWTPs and concentrations of DEHP, DiNP and DiDP was significant.

Ecuador publications in the Science Citation Index Expanded: institutions, subjects, citation and collaboration patterns

Introduction: Ecuador, a country of 17 million inhabitants with a medium human development index of 0.75, has a small scientific productivity in relation to its size and population. Objective: To analyze Ecuador publications in the Science Citation Index Expanded, focusing on productivity, subjects, institutions, citations, and trends. Methods: We analyzed scientific publications by authors from Ecuador from 1900 to 2017 in the Science Citation Index Expanded and compared it with other tropical countries. Results: We found 16 document types (7 806 articles). The three most productive institutions were Universidad San Francisco de Quito, Pontificia Universidad Católica del Ecuador, and Escuela Politécnica Nacional. USA and Spain were the most frequent collaborating countries. Most articles were in English and ecology, botany, and zoology were common, but no field produced over 8 % of articles. However, ecology represents 11.3 % of the total citations. The most cited papers in the database were from large international biology and physics projects with minimal participation of Ecuadorean scientists. Article citations occurs mostly after the SCI stops counting. Conclusion: Science in Ecuador is growing but needs to greatly increase collaboration among Ecuadorean institutions to reduce its dependence on foreing projects. However, this study did not include articles published by the hundreds of Ecuadorean journals not covered by the SCI Expanded.

Introducción: Ecuador es un país con 17 millones de habitantes y un indice de desarrollo humano medio de 0.75, pero tiene una baja producción científica en relación con su tamaño y población. Objectivo: Analizar las publicaciones de Ecuador en el Índice de Citación de Ciencia enfocándose en la productividad, categorías, instituciones, citaciones y tendencias. Métodos: En la base de datos Science Citation Index analizamos las publicaciones científicas de autores con dirección ecuatoriana desde 1900 hasta 2017 y comparamos los resultados con otros países tropicales. Resultados: Encontramos 16 tipos de documentos (7 806 artículos). Las tres instituciones más productivas fueron Universidad San Francisco de Quito, Pontificia Universidad Católica del Ecuador, y Escuela Politécnica Nacional. Estados Unidos y España fueron los países con colaboración más frecuente. La mayoría de los artículos fueron escritos en inglés con Ecología, Botánica y Zoología como las categorías más comunes, pero ninguna supera una producción del 8 % del total de las publicaciones. Sin embargo, Ecología representa el 11.3 % del total de citaciones. Los artículos más citados en esa base de datos fueron de grandes equipos internacionales en Biología y en Física, con poca participación de científicos ecuatorianos. La mayoría de las citas se dan después de que el SCI detiene el conteo. Conclusión: La ciencia en Ecuador está creciendo, pero se necesita aumentar la colaboración entre las instituciones ecuatorianas para reducir la dependencia de proyectos extranjeros. Se debe considerar que este estudio no incluyó los artículos publicados por centenares de revistas ecuatorianas que no son cubiertas por el Science Citation Index.

Global performance and development on sustainable city based on natural science and social science research: A bibliometric analysis.

Sustainable city has been a global concern in recent years, involving natural science and social science research. However, few studies have attempted to map the global research of sustainable city. This study aimed to reveal the global performance and development of sustainable city research during 1992 to 2016, using a bibliometric analysis based on Science Citation Index Expanded (SCI-EXPANDED) and Social Sciences Citation Index (SSCI) on Web of Science. Research performance, research emphases and trends were illustrated by bibliometric indicators and newly developed visualization tools, like Science of Science (Sci2), Gephi, and alluvial diagram. Sustainable city research in natural science and social science were well-matched in quantity, and had similar and different characteristics in terms of different investigated aspects. China ranked first in natural science research, while the USA was the leading country in social science research. More categories and clusters were involved in sustainable city field, indicating more interdisciplinary integration was undergoing. Energy and fuels in natural science field, and interdisciplinary applications mathematics in social science field started to attract more attention. Statistical analysis of words in title, author keywords, and KeyWords Plus has been investigated to monitor the development of sustainable city research. Finally, three critical issues in sustainable city research based on the clues by the analysis of these keywords were reviewed and discussed.

Mapping of drinking water research: a bibliometric analysis of research output during 1992-2011.

A bibliometric analysis based on the Science Citation Index Expanded from the Web of Science was carried out to provide insights into research activities and tendencies of the global drinking water from 1992 to 2011. Study emphases included performance of publication covering annual outputs, mainstream journals, Web of Science categories, leading countries, institutions, research tendencies and hotspots. The results indicated that annual output of the related scientific articles increased steadily. Water Research, Environmental Science & Technology, and Journal American Water Works Association were the three most common journals in drinking water research. The USA took a leading position out of 168 countries/territories, followed by Japan and Germany. A summary of the most frequently used keywords obtained from words in paper title analysis, author keyword analysis and KeyWords Plus analysis provided the clues to discover the current research emphases. The mainstream research related to drinking water was water treatment methods and the related contaminants. Disinfection process and consequent disinfection by-products attracted much attention. Ozonation and chlorination in disinfection, and adsorption were common techniques and are getting popular. Commonly researched drinking water contaminants concerned arsenic, nitrate, fluoride, lead, and cadmium, and pharmaceuticals emerged as the frequently studied contaminants in recent years. Disease caused by contaminants strongly promoted the development of related research.