ABSTRACT
BACKGROUND AND OBJECTIVE: Cytologic screening of asymptomatic high risk individuals can detect curable esophageal carcinomas and has been used for several decades. However, the sensitivity of such screening is relatively low, which limits its wide use and development. This study was to investigate the utility of liquid-based cytology in esophageal carcinoma screening. METHODS: A mass screening of esophageal carcinoma was performed for asymptomatic residents in Yaocun County, Linzhou City, Henan Province, China. Esophageal biopsy samples were put into a liquid buffer for cytologic diagnosis and subsequent endoscopic biopsies were made on all subjects. Cytologic categories were adapted from criteria of the Bethesda system (TBS). RESULTS of liquid-based cytology were compared with those from endoscopic biopsy. The sensitivity and the specificity of liquid-based cytology were evaluated. RESULTS: Carcinomas in situ and carcinomas were identified in 17 (2.4%) of 710 subjects. Measured by ASC/AGC (atypical squamous cells or atypical glandular cells) as the detection threshold, the sensitivity and the specificity of liquid-based cytology were 76.5% and 76.0%, respectively. CONCLUSION: In a hospital with a high level of conventional cytology, liquid-based technique can be used widely since the work load of reading slides may greatly decrease, although this technique do not significantly improve the sensitivity of screening.
Subject(s)
Adenocarcinoma/diagnosis , Carcinoma in Situ/diagnosis , Carcinoma, Squamous Cell/diagnosis , Cytodiagnosis/methods , Esophageal Neoplasms/diagnosis , Adult , Aged , China , Humans , Mass Screening , Middle Aged , Precancerous Conditions/diagnosis , Sensitivity and SpecificitySubject(s)
Cervix Uteri/pathology , Endometrial Neoplasms/diagnosis , Endometrial Neoplasms/pathology , Adult , Carcinoma, Endometrioid/diagnosis , Carcinoma, Endometrioid/pathology , Diagnosis, Differential , Endometrial Stromal Tumors/diagnosis , Endometrial Stromal Tumors/pathology , Female , Humans , Middle AgedABSTRACT
OBJECTIVE: To evaluate the value of p16INK4a immuncytochemical examination in cytological screening of cervical carcinoma and precancerous lesions. METHODS: p16JNK4a immuncytochemical detection was performed on 220 specimens remaining from liquid-based cytology, followed up by biopsy histology , and compared with the results of high-risk human papillomavirus ( HR - HPV ) DNA tests . Results In patients with cytological diagnosis of squamous cell carcinoma( SCC) , high-grade squamous intraepithelial lesion (HSIL) , low-grade squamous intraepithelial lesion (LSIL) , atypical squamous cells-cannot exclude HSIL (ASC-H) , and atypical squamous cells of undetermined significance (ASC-US) , the positive rates of p16INK4a were 100.0% (7/7), 92. 2% (107/116) , 24. 3% (17/70) , 100. 0% (14/14) and 36.4% (4/ 11) , respectively. In 111 of the 150 p6INK4a positive cases, we found 97 (87.4% ) cases which had biopsy diagnosises of > or =CIN2, but none in 18 of 70 p16INK4a negative cases was. The difference in the positive rates for p16INK4a between cervical intraepithelial neoplasia (CIN) 1 and > or =CIN2 lesions had statistical significance (P < 0. 01) , whereas for HR-HPV DNA test it was not. CONCLUSION: p16LNK4a is over-expressed in a HSIL, and it may be useful in cytological screening of high risk patients.
