ABSTRACT
HLA-DRB1*12:01:12 differs from HLA-DRB1*12:01:01:01 by one nucleotide in exon 2.
Subject(s)
East Asian People , HLA-DRB1 Chains , Humans , Alleles , Base Sequence , HLA-DRB1 Chains/genetics , NucleotidesABSTRACT
HLA-C*01:239 differs from HLA-C*01:02:01:01 by one nucleotide in exon 4.
Subject(s)
HLA-C Antigens , Humans , Alleles , East Asian People , HLA-C Antigens/genetics , Nucleotides , Sequence Analysis, DNAABSTRACT
HLA-DRB1*14:246 differs from HLA-DRB1*14:18 by one nucleotide in exon 2.
Subject(s)
East Asian People , Nucleotides , Humans , HLA-DRB1 Chains/genetics , AllelesABSTRACT
Mastering the SNP content in the HLA region can be based on it to judiciously select unrelated donor stem cells with preferable MHC matching to lower postoperative complications. Herein, quantitative PCR-based primers and probes were designed for 10 transplants outcome-associated SNP loci with two-allelic polymorphism, and then a new detection system ("HLA-10-SNP") was established. Compared with Sanger sequencing, its accuracy has been proven to reach 100%. Additionally, fluorescent PCR typing of 10 important SNPs via this system expressed excellent repeatability (sensitivity, 20 ng). Overall, the new system achieves single-sample classification precision and easily distinguishable results, equipped with the advantages of simple, rapid, accurate, and effective, promising to acquire widespread popularization and application in clinical settings.
Subject(s)
Polymorphism, Single Nucleotide , Unrelated Donors , Alleles , Histocompatibility Testing , Humans , Real-Time Polymerase Chain ReactionABSTRACT
BACKGROUND: The killer cell immunoglobulin-like receptor (KIR), which mediates the killing function of NK cells, is an attractive candidate for adoptive cellular therapy. The ethnic distribution for China provides a unique opportunity to investigate KIR gene distribution. AIM: The aim of this study was to explore the relationship between population history and the rapidly evolving KIR genetic diversity. SUBJECTS AND METHODS: 8050 Chinese donors from 184 hospitals were included to analyse frequency, haplotype, and B-content data of 16 KIR genes, by PCR-SSP for KIR genotyping. RESULTS: KIR gene carrier frequencies were found similar to those observed in other studies on Han, but different from Thais, Japanese, Africans, and populations of West Eurasian ancestry. High-frequency KIR genotype profiles found in the present population were consistent with other studies on Han populations but different from those conducted on other cohorts. The majority of our cohort carried group A KIR gene motifs. Additionally, populations with similar geographic locations in China were shown clustered together, while Hainan and Xinjiang provinces were slightly separated from these. CONCLUSION: The distribution of KIR genes varies by geographic region, and different ethnic groups may be a confounding factor of KIR diversity.
