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Sci Rep ; 6: 21651, 2016 Feb 22.
Article in English | MEDLINE | ID: mdl-26898479

ABSTRACT

PcINF1 was previously found to induce pepper defense response by interacting with SRC2-1, but the underlying mechanism remains uninvestigated. Herein, we describe the involvement of SGT1 in the PcINF1/SRC2-1-induced immunity. SGT1 was observed to be up-regulated by Phytophthora capsici inoculation and synergistically transient overexpression of PcINF1/SRC2-1 in pepper plants. SGT1-silencing compromised HR cell death, blocked H2O2 accumulation, and downregulated HR-associated and hormones-dependent marker genes' expression triggered by PcINF1/SRC2-1 co-overexpression. The interaction between SRC2-1 and SGT1 was found by the yeast two hybrid system and was further confirmed by bimolecular fluorescence complementation and co-immunoprecipitation analyses. The SGT1/SRC2-1 interaction was enhanced by transient overexpression of PcINF1 and Phytophthora capsici inoculation, and SGT1-silencing attenuated PcINF1/SRC2-1 interaction. Additionally, by modulating subcellular localizations of SRC2-1, SGT1, and the interacting complex of SGT1/SRC2-1, it was revealed that exclusive nuclear targeting of the SGT1/SRC2-1 complex blocks immunity triggered by formation of SGT1/SRC2-1, and a translocation of the SGT1/SRC2-1 complex from the plasma membrane and cytoplasm to the nuclei upon the inoculation of P. capsici. Our data demonstrate that the SGT1/SRC2-1 interaction, and its nucleocytoplasmic partitioning, is involved in pepper's immunity against P. capsici, thus providing a molecular link between Ca(2+) signaling associated SRC2-1 and SGT1-mediated defense signaling.


Subject(s)
Capsicum/genetics , Disease Resistance/genetics , Glucosyltransferases/genetics , Phytophthora/genetics , Plant Diseases/genetics , Plant Proteins/genetics , Proteins/genetics , Active Transport, Cell Nucleus , Calcium Signaling , Capsicum/immunology , Capsicum/microbiology , Cell Death , Cell Membrane/metabolism , Cell Nucleus/metabolism , Gene Expression Regulation, Plant , Gene Silencing , Glucosyltransferases/immunology , Host-Pathogen Interactions , Phytophthora/growth & development , Phytophthora/metabolism , Plant Diseases/immunology , Plant Diseases/microbiology , Plant Leaves/genetics , Plant Leaves/immunology , Plant Leaves/microbiology , Plant Proteins/immunology , Protein Binding , Protein Transport , Proteins/metabolism , Two-Hybrid System Techniques
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