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1.
Org Biomol Chem ; 21(36): 7290-7294, 2023 Sep 20.
Article in English | MEDLINE | ID: mdl-37650516

ABSTRACT

Electrophilic bromocyclization reactions are widely used as key steps in the synthesis of diverse functionalized tetrahydrofuroindolines and hexahydropyrroloindolines. However, the direct dibromination variants of these reactions for the synthesis of 3,5-dibromoindolines remain undeveloped. Here, we report a protonic-acid-promoted electrooxidative protocol for the dearomative C3,C5-dibromocyclizations of tryptophol and tryptamine derivatives. This electrosynthetic approach, which enables direct selective construction of heterocyclic 3a,5a-dibromoindolines with inexpensive, non-hazardous NaBr as both the electrolyte and Br source, provides a convenient, practical method for the late-stage 3,5-diversification of heterocyclic indolines.

2.
Org Lett ; 24(18): 3358-3362, 2022 05 13.
Article in English | MEDLINE | ID: mdl-35503733

ABSTRACT

We report a safe and convenient open-flask copper-catalyzed selective oxidation/functionalization methodology for tetrahydrocarbolines and tetrahydro-ß-carbolines that employs atmospheric O2 as the terminal oxidant. The system is applicable to oxidative rearrangement of tetrahydro-ß-carbolines, tetrahydrocarboline oxidation to α-alkoxy carbazoles and spirooxindoles, and Witkop oxidation. Mechanistic experiments indicated that a single-electron oxidation process is responsible for the tunable selectivity control. This copper-catalysis protocol represents a significant advance in the field of indole oxidation.


Subject(s)
Carbolines , Copper , Catalysis , Molecular Structure , Oxidation-Reduction
3.
J Org Chem ; 86(23): 17164-17172, 2021 12 03.
Article in English | MEDLINE | ID: mdl-34709032

ABSTRACT

The first transition metal catalytic one-step synthesis of the 3a, 3a'-bispyrrolidino [2,3-b] indoline scaffold via tandem cyclization/dimerization of tryptamines has been realized with the environmentally friendly O2/air as the sole oxidant. Different from the traditional direct oxidation of indole "N-H" group by excess amount of metal salts, a copper-catalyzed oxidative cyclization reaction is developed for the formation of the radical pyrrolidinoindoline intermediate in the current strategy. The robustness and practicality of this methodology is demonstrated by the step-economic, divergent total synthesis of natural products (±)-folicanthine and meso-folicanthine.


Subject(s)
Copper , Tryptamines , Catalysis , Cyclization , Dimerization , Indoles , Oxidants
4.
Front Biosci (Landmark Ed) ; 24(4): 712-722, 2019 03 01.
Article in English | MEDLINE | ID: mdl-30844707

ABSTRACT

Although not without controversy, as a general trend, the human sperm count is declining world-wide. One major reason for such a decline is an increase in the human life-span.  According to the life history tradeoff theory, fecundity is inversely related to the lifespan; the longer the lifespan, the lower the fecundity. This is essential to the maintainance of diversity and balance of different species. Such a corrleation validated by experimental data that show that the extension of life in Caenorhabditis elegans, Drosophila and Rodents is  associated with reduction in fecundity. The demographic data from a public data source, shows that the total fertility rate is positively correlated with the infant death rate, it is inversely correlated with the life expectancy. We postulate that the fall in spermatogenesis might be regulated by the neuroendocrine system that underlie human longevity.


Subject(s)
Life Expectancy , Oligospermia/epidemiology , Sperm Count , Animals , Birth Rate , Caenorhabditis elegans , Denmark , Drosophila melanogaster , Environmental Pollutants , Escherichia coli/physiology , Fertility , Global Health , Humans , Life Style , Longevity , Male , Models, Theoretical , Mycobacterium leprae/physiology , Mycobacterium tuberculosis/physiology , Rats , Yeasts/physiology
5.
Oncol Lett ; 14(4): 4722-4728, 2017 Oct.
Article in English | MEDLINE | ID: mdl-29085472

ABSTRACT

Liver cancer is the one of most common types of cancer and the 2nd cause of cancer-associated mortalities worldwide. Establishing appropriate animal models of liver cancer is essential for basic and translational studies. The present study evaluated the effects of the combined use of alcohol with a conventional chemical-induced mouse liver cancer model. The treatment of alcohol/diethylnitrosamine (DEN)/carbon tetrachloride (CCl4) in the mice of experimental groups resulted in a series of pathological changes in the liver. Liver inflammation, fibrosis, cirrhosis and hepatocellular carcinoma were identified, and this method used less time (1-5 months) for inducement compared with the conventional chemical-induced method alone. In addition, murine α-fetoprotein (mAFP) was expressed throughout and ultrastructural features met the criteria for liver cancer. Fatty degeneration of pancreas, reduced blood glucose levels, and increased spleen weight were observed. These results indicated that an AFP-secreting hepatocellular carcinoma model of BALB/c mouse was successfully developed. The disease process and morphological changes met the criterion of the liver cancer process. Therefore the model developed in the present study may be an ideal animal model for studying the occurrence and development of liver cancer.

6.
Med Sci Monit ; 23: 3932-3941, 2017 Aug 15.
Article in English | MEDLINE | ID: mdl-28808223

ABSTRACT

BACKGROUND The aim of this study was to investigate the effects of metastasis-associated protein 1 (MTA1) deficiency during angiogenesis of pulmonary alveolar capillaries in mice and to determine the molecular mechanisms involved. MATERIAL AND METHODS The expressions of MTA1, CD34, vascular endothelial growth factor (VEGF), alpha smooth muscle actin (α-SMA), and HIF-1α were analyzed in the lungs of MTA1-knockout (KO) and wild-type mice at embryonic day 18.5 and 2 months by quantitative PCR, immunoblotting, and immunohistochemistry. The morphological changes were investigated during pulmonary alveolar capillary formation. The heart weight/body weight (HW/BW) ratio and the size of the right ventricular wall cardiomyocytes were also measured. Regulation of MTA1 on HIF-1α was determined in vitro. RESULTS MTA1 deficiency reduced the number of pulmonary alveolar capillaries compared to the wild-type mice. MTA1-KO mice exhibited a decreased expression of HIF-1α and VEGF in the lungs. The retarded growth of the MTA1-KO mice was also noticed during the first week after birth. Accordingly, MTA1 deficiency resulted in increased infant mortality. In surviving adult mice, MTA1 deficiency induced myocardial hypertrophy, highlighted by an increased heart weight/body weight ratio and larger cardiomyocytes. In cultured cells, HIF-1α and VEGF levels were significantly upregulated upon MTA1 overexpression, suggesting a close relationship between all 3 molecules. CONCLUSIONS MTA1 participates in the formation of pulmonary capillaries via stabilization of HIF-1α. This finding sheds new light on the function of MTA1 in lung development, opening new avenues for the diagnosis/treatment of related pulmonary diseases.


Subject(s)
Pulmonary Alveoli/blood supply , Transcription Factors/deficiency , Actins/metabolism , Animals , Antigens, CD34/metabolism , Capillaries/metabolism , Hypoxia-Inducible Factor 1, alpha Subunit/metabolism , Immunohistochemistry , Mice , Mice, Knockout , Myocytes, Cardiac/metabolism , Neovascularization, Physiologic/physiology , Repressor Proteins/genetics , Repressor Proteins/metabolism , Trans-Activators , Transcription Factors/genetics , Transcription Factors/metabolism , Transcriptional Activation , Vascular Endothelial Growth Factor A/metabolism
7.
Genes Dis ; 4(1): 16-18, 2017 Mar.
Article in English | MEDLINE | ID: mdl-30258903

ABSTRACT

Epithelial-mesenchymal transition (EMT) is the phenotypic transition of epithelial cells to mesenchymal cells characterized by loss of epithelial markers, loss of intercellular adherence and acquirement of mesenchymal cell markers and increased locomotive ability. EMT is widely considered to be a gene regulated process necessary for cancer metastasis. Yet it is a highly controversial issue. We here propose that EMT is an environmentally induced cell behavior. It is the mimicry of their living environment. It is a survival strategy, a way of immune escape. We also propose here that the epithelial cell markers may functionally act as tumor antigens since in the mesenchymal surroundings there are no other structures bearing the same antigens as epithelial cells.

8.
Genes Dis ; 4(3): 154-158, 2017 Sep.
Article in English | MEDLINE | ID: mdl-30258917

ABSTRACT

There are no eternal individual lives so life continues by relaying with reproduction. Consequently, lifespan and fecundity are two essential genetic traits of life. The life history tradeoffs theory holds that there is an inverse relationship between lifespan and fecundity. This paper proposes two new concepts, i.e., "lifespan of pathogens" and treatment of infections by "antibiogenesis". The lifespan of pathogens is the time limitation of those tiny lives just as other large creatures. Notably, the lifespan of bacterium is the time interval from the cell division by which it is produced to next division by then its life ends and transforms to two new lives, or dies. Antibiogenesis means inhibiting generation of new lives. By the principle of life history tradeoffs, the lifespan of pathogens determines the speed of their proliferations and consequently the modality of infection. The treatment principle of antibiogenesis requires the duration of treatment to be determined by the lifespan of infected pathogens. The life history tradeoffs theory and the two concepts are helpful to understanding the pathobiology and shaping the clinical aspects of infectious diseases.

9.
Int J Clin Exp Pathol ; 10(7): 7743-7749, 2017.
Article in English | MEDLINE | ID: mdl-31966621

ABSTRACT

Immune surveillance is a highly controversial subject in both the field of immunology and cancer biology. On one hand, in spite of extensive studies, there is no cancer specific antigens identified. Yet, the organisms do exert immune response to tumors. On the other hand, it is believed that immune surveillance suppresses tumorigenesis by eradicating mutated cells. However, it is also widely known that tumorigenesis is promoted by inflammation, which is in nature immune reaction. In the present study, we tried to find immune cells in early tumor lesions for the supportive or negative evidence of immune surveillance. We used immunohistochemistry to observe the localization and distribution of immune cells in the in situ carcinoma lesions and in the invasive cancer of breast. Interestingly, we did not see immune cells in either ductal carcinoma in situ (DCIS) or lobular carcinoma in situ (LCIS) of breast, the two basic supposed early cancer forms. In contrast, we observed extensive infiltration of immune cells in the invasive breast cancer, and close contact between immune cells and tumor cells. Based on these findings, we propose that the tumor antigens of breast cancer are not derived from the gene mutation or amplification such as HER2, but rather from misplacement of epithelial cells in the mesenchymal tissue. To avoid being targeted by the immune system, the carcinoma cells exert epithelial-mesenchymal transition (EMT). Therefore, immunosurveillance could be regarded as preventing the intrusion of epithelial cells to mesenchymal tissues, and EMT is a form of immune escape by the strategy of mimicry.

10.
Gene ; 582(2): 112-21, 2016 May 15.
Article in English | MEDLINE | ID: mdl-26869315

ABSTRACT

Metastatic associated proteins (MTA) are integrators of upstream regulatory signals with the ability to act as master coregulators for modifying gene transcriptional activity. The MTA family includes three genes and multiple alternatively spliced variants. The MTA proteins neither have their own enzymatic activity nor have been shown to directly interact with DNA. However, MTA proteins interact with a variety of chromatin remodeling factors and complexes with enzymatic activities for modulating the plasticity of nucleosomes, leading to the repression or derepression of target genes or other extra-nuclear and nucleosome remodeling and histone deacetylase (NuRD)-complex independent activities. The functions of MTA family members are driven by the steady state levels and subcellular localization of MTA proteins, the dynamic nature of modifying signals and enzymes, the structural features and post-translational modification of protein domains, interactions with binding proteins, and the nature of the engaged and resulting features of nucleosomes in the proximity of target genes. In general, MTA1 and MTA2 are the most upregulated genes in human cancer and correlate well with aggressive phenotypes, therapeutic resistance, poor prognosis and ultimately, unfavorable survival of cancer patients. Here we will discuss the structure, expression and functions of the MTA family of genes in the context of cancer cells.


Subject(s)
Gene Expression Regulation, Neoplastic , NM23 Nucleoside Diphosphate Kinases/chemistry , NM23 Nucleoside Diphosphate Kinases/genetics , Alternative Splicing/genetics , Animals , Humans , Multigene Family , Mutation/genetics , NM23 Nucleoside Diphosphate Kinases/metabolism , Protein Structure, Tertiary
11.
Mol Clin Oncol ; 3(6): 1199-1202, 2015 Nov.
Article in English | MEDLINE | ID: mdl-26807220

ABSTRACT

Over several years, scientists investigating cancer have focused their efforts on elucidating the mechanisms underlying cancer metastasis, with the aim of finding a way to inhibit this process. These mechanisms, however, only explain the process of cancer metastasis, but do not explain why cancer would metastasize in the first place. Cancer metastasizes due to several factors, namely attack by the immune system, lack of oxygen and necessary nutrients, large amounts of lactic acid produced by glycolysis and increased cell death. Therefore, the majority of the presently available treatments for cancer also bear the potential to induce metastasis. Thus, it is crucial in medical practice to minimize the risk of cancer metastasis during a time when there are no effective means to inhibit this process.

12.
Cancer Metastasis Rev ; 33(4): 1001-9, 2014 Dec.
Article in English | MEDLINE | ID: mdl-25332145

ABSTRACT

Gene mutation's role in initiating carcinogenesis has been controversial, but it is consensually accepted that both carcinogenesis and cancer metastasis are gene-regulated processes. MTA1, a metastasis-associated protein, has been extensively researched, especially regarding its role in cancer metastasis. In this review, I try to elucidate MTA1's role in both carcinogenesis and metastasis from a different angle. I propose that MTA1 is a stress response protein that is upregulated in various stress-related situations such as heat shock, hypoxia, and ironic radiation. Cancer cells are mostly living in a stressful environment of hypoxia, lack of nutrition, and immune reaction attacks. To cope with all these stresses, MTA1 expression is upregulated, plays a role of master regulator of gene expression, and helps cancer cells to survive and migrate out of their original dwelling.


Subject(s)
Carcinogenesis/genetics , Heat-Shock Response/genetics , Histone Deacetylases/genetics , Neoplasms/genetics , Repressor Proteins/genetics , Cell Hypoxia/genetics , Gene Expression Regulation, Neoplastic , Histone Deacetylases/biosynthesis , Humans , Neoplasm Metastasis , Neoplasms/drug therapy , Neoplasms/radiotherapy , Radiation, Ionizing , Repressor Proteins/biosynthesis , Trans-Activators
13.
Chin J Cancer ; 33(2): 47-50, 2014 Feb.
Article in English | MEDLINE | ID: mdl-24417874

ABSTRACT

In the research community, resistance to apoptosis is often considered a hallmark of cancer. However, pathologists who diagnose cancer via microscope often see the opposite. Indeed, increased apoptosis and mitosis are usually observed simultaneously in cancerous lesions. Studies have shown that increased apoptosis is associated with cancer aggressiveness and poor clinical outcome. Furthermore, overexpression of Bcl-2, an antiapoptotic protein, is linked with better survival of cancer patients. Conversely, Bax, CD95, Caspase-3, and other apoptosis-inducing proteins have been found to promote carcinogenesis. This notion of the role of apoptosis in cancer is not new; cancer cells were found to be short-lived 88 years ago. Given these observations, resistance to apoptosis should not be considered a hallmark of cancer.


Subject(s)
Apoptosis , Biomarkers, Tumor/metabolism , Carcinogenesis , Neoplasms/pathology , Animals , Apoptosis/physiology , Carcinogenesis/metabolism , Caspase 3/metabolism , Humans , Lymphoma, B-Cell/metabolism , Lymphoma, B-Cell/pathology , Neoplasms/metabolism , Proto-Oncogene Proteins c-bcl-2/metabolism , Treatment Outcome , bcl-2-Associated X Protein/metabolism , fas Receptor/metabolism
14.
Hepatology ; 59(5): 1850-63, 2014 May.
Article in English | MEDLINE | ID: mdl-24002871

ABSTRACT

UNLABELLED: The MYC oncogene is overexpressed in hepatocellular carcinoma (HCC) and has been associated with widespread microRNA (miRNA) repression; however, the underlying mechanisms are largely unknown. Here, we report that the c-Myc oncogenic transcription factor physically interacts with enhancer of zeste homolog 2 (EZH2), a core enzymatic unit of polycomb repressive complex 2 (PRC2). Furthermore, miR-101, an important tumor-suppressive miRNA in human hepatocarcinomas, is epigenetically repressed by PRC2 complex in a c-Myc-mediated manner. miR-101, in turn, inhibits the expression of two subunits of PRC2 (EZH2 and EED), thus creating a double-negative feedback loop that regulates the process of hepatocarcinogenesis. Restoration of miR-101 expression suppresses multiple malignant phenotypes of HCC cells by coordinate repression of a cohort of oncogenes, including STMN1, JUNB, and CXCR7, and further increases expression of endogenous miR-101 by inhibition of PRC2 activation. In addition, co-overexpression of c-Myc and EZH2 in HCC samples was closely associated with lower expression of miR-101 (P < 0.0001) and poorer prognosis of HCC patients (P < 0.01). CONCLUSIONS: c-Myc collaborates with EZH2-containing PRC2 complex in silencing tumor-suppressive miRNAs during hepatocarcinogenesis and provides promising therapeutic candidates for human HCC.


Subject(s)
Carcinoma, Hepatocellular/genetics , Gene Expression Regulation, Neoplastic , Gene Silencing , Liver Neoplasms/genetics , MicroRNAs/physiology , Proto-Oncogene Proteins c-myc/physiology , Animals , Carcinoma, Hepatocellular/mortality , Carcinoma, Hepatocellular/pathology , DNA Methylation , Enhancer of Zeste Homolog 2 Protein , Humans , Liver Neoplasms/mortality , Liver Neoplasms/pathology , Mice , Mice, Inbred BALB C , MicroRNAs/antagonists & inhibitors , Polycomb Repressive Complex 2/metabolism , Polycomb Repressive Complex 2/physiology , Receptors, CXCR/physiology
15.
PLoS One ; 8(10): e77795, 2013.
Article in English | MEDLINE | ID: mdl-24167583

ABSTRACT

BACKGROUND: Airway remodeling is a repair process that occurs after injury resulting in increased airway hyper-responsiveness in asthma. Thymic stromal lymphopoietin (TSLP), a vital cytokine, plays a critical role in orchestrating, perpetuating and amplifying the inflammatory response in asthma. TSLP is also a critical factor in airway remodeling in asthma. OBJECTIVES: To examine the role of TSLP-induced cellular senescence in airway remodeling of asthma in vitro and in vivo. METHODS: Cellular senescence and airway remodeling were examined in lung specimens from patients with asthma using immunohischemical analysis. Both small molecule and shRNA approaches that target the senescent signaling pathways were used to explore the role of cellular senescence in TSLP-induced airway remodeling in vitro. Senescence-Associated ß-galactosidase (SA-ß-Gal) staining, and BrdU assays were used to detect cellular senescence. In addition, the Stat3-targeted inhibitor, WP1066, was evaluated in an asthma mouse model to determine if inhibiting cellular senescence influences airway remodeling in asthma. RESULTS: Activation of cellular senescence as evidenced by checkpoint activation and cell cycle arrest was detected in airway epithelia samples from patients with asthma. Furthermore, TSLP-induced cellular senescence was required for airway remodeling in vitro. In addition, a mouse asthma model indicates that inhibiting cellular senescence blocks airway remodeling and relieves airway resistance. CONCLUSION: TSLP stimulation can induce cellular senescence during airway remodeling in asthma. Inhibiting the signaling pathways of cellular senescence overcomes TSLP-induced airway remodeling.


Subject(s)
Airway Remodeling , Asthma/metabolism , Immunoglobulins/metabolism , Receptors, Cytokine/metabolism , Adolescent , Adult , Animals , Asthma/drug therapy , Asthma/pathology , Cell Cycle Checkpoints/drug effects , Cellular Senescence/drug effects , Female , Humans , Male , Mice , Mice, Inbred BALB C , Pyridines/pharmacology , STAT3 Transcription Factor/antagonists & inhibitors , Signal Transduction/drug effects , Tyrphostins/pharmacology
16.
J Cell Mol Med ; 17(7): 921-6, 2013 Jul.
Article in English | MEDLINE | ID: mdl-23741988

ABSTRACT

Cancers are thought to be the result of accumulated gene mutations in cells. Carcinomas, which are cancers arising from epithelial tissues usually go through several stages of development: atypical hyperplasia, carcinoma in situ and then invasive carcinoma, which might further metastasize. However, we think that the present pathological data are enough to prove that there might be an alternative way of carcinogenesis. We propose that majority of invasive cancers arise in the connective tissue stroma de novo, from the misplaced epithelial stem cells which come to the wrong land of connective tissue stroma by accident. The in situ carcinomas, which are mostly curable, should not be considered genuine cancer, but rather as quasi-cancer. We design this new theory of carcinogenesis as the stem cell misplacement theory (SCMT). Our SCMT theory chains together other carcinogenesis theories such as the inflammation-cancer chain, the stem cell theory and the tissue organization field theory. However, we deny the pathway of somatic mutation theory as the major pathway of carcinogenesis.


Subject(s)
Carcinogenesis/pathology , Gene Expression Regulation, Neoplastic , Neoplasm Invasiveness , Neoplasms/pathology , Stem Cells/cytology , Animals , Basement Membrane , Breast Neoplasms/pathology , Carcinoma, Ductal, Breast/pathology , Connective Tissue/pathology , Disease Progression , Epithelial Cells/cytology , Epithelial-Mesenchymal Transition , Female , Humans , Inflammation/pathology , Mice , Mutation , Neoplasm Metastasis , Telomerase/metabolism
17.
Cancer Res ; 73(12): 3761-70, 2013 Jun 15.
Article in English | MEDLINE | ID: mdl-23580571

ABSTRACT

Overexpression of the prometastatic chromatin modifier protein metastasis tumor antigen 1 (MTA1) in human cancer contributes to tumor aggressiveness, but the role of endogenous MTA1 in cancer has not been explored. Here, we report the effects of selective genetic depletion of MTA1 in a physiologically relevant spontaneous mouse model of breast cancer pulmonary metastasis. We found that MTA1 acts as a mandatory modifier of breast-to-lung metastasis without effects on primary tumor formation. The underlying mechanism involved MTA1-dependent stimulation of STAT3 transcription through action on the MTA1/STAT3/Pol II coactivator complex, and, in turn, on the expression and functions of STAT3 target genes including Twist1. Accordingly, we documented a positive correlation between levels of MTA1 and STAT3 in publicly available breast cancer data sets. Together, our findings reveal an essential modifying role of the physiologic level of MTA1 in supporting pulmonary metastasis of breast cancer.


Subject(s)
Lung Neoplasms/genetics , Mammary Neoplasms, Animal/genetics , STAT3 Transcription Factor/genetics , Transcription Factors/genetics , Animals , Blotting, Western , Breast Neoplasms/genetics , Breast Neoplasms/metabolism , Breast Neoplasms/pathology , Cell Movement/genetics , Cells, Cultured , DNA Polymerase II/metabolism , Female , Gene Expression Regulation, Neoplastic , Humans , Lung Neoplasms/metabolism , Lung Neoplasms/secondary , Mammary Neoplasms, Animal/metabolism , Mammary Neoplasms, Animal/pathology , Mice , Mice, 129 Strain , Mice, Knockout , Nuclear Proteins/genetics , Nuclear Proteins/metabolism , Protein Binding , RNA Interference , Repressor Proteins , Reverse Transcriptase Polymerase Chain Reaction , STAT3 Transcription Factor/metabolism , Trans-Activators , Transcription Factors/metabolism , Transcription, Genetic , Twist-Related Protein 1/genetics , Twist-Related Protein 1/metabolism
18.
J Cell Mol Med ; 17(1): 205-11, 2013 Jan.
Article in English | MEDLINE | ID: mdl-23305095

ABSTRACT

Cancer has been considered to be the result of accumulated gene mutations, which result in uncontrolled cell proliferations for a long time. Cancers are also regarded to be capable of immune evasion. Furthermore, resistance to apoptosis was recognized as an important trait of cancer in the last score of years. However, there are numerous paradoxical issues in this whole set of theory. For example, there is no known set of genes of which mutations are responsible for human cancers. As for the trait of 'resistance to apoptosis', the fact is that cancer has increased frequency of apoptosis. The more malignant the tumour is, the more apoptosis shows. In this study, we propose a new theory that apoptosis plays a key role in the malignant progression and metastasis of cancer. The growth of tumour is the difference between tumour cell proliferation and attrition plus the hyperplastic growth of stroma. Increased and unpreventable death caused by innate or environmental factors such as ischaemia and inflammation drives the tumour cells to proliferate relentlessly, move to new lands to establish colonies. In short, increased cell death is the origin of malignancy.


Subject(s)
Apoptosis Regulatory Proteins/genetics , Apoptosis/genetics , Genes, Neoplasm , Neoplasms/pathology , Apoptosis Regulatory Proteins/metabolism , Cell Count , Cell Cycle/genetics , Cell Proliferation , Gene Expression Regulation, Neoplastic , Humans , Mutation , Neoplasm Metastasis , Neoplasms/genetics , Neoplasms/metabolism , Stromal Cells/metabolism , Stromal Cells/pathology
19.
Pigment Cell Melanoma Res ; 26(1): 67-77, 2013 Jan.
Article in English | MEDLINE | ID: mdl-23020925

ABSTRACT

The paired box homeotic gene 3 (PAX3) is a crucial regulator for the maintenance of melanocytic progenitor cells and has a poorly defined role in melanoma. To understand how PAX3 affects melanocyte and melanoma proliferation, we identified potential PAX3 downstream targets through gene expression profiling. Here, we identify T-box 2 (TBX2), a key developmental regulator of cell identity and an antisenescence factor in melanoma, as a directly regulated PAX3 target. We also found that TBX2 is involved in the survival of melanoma cells and is overexpressed in some melanoma specimens. The identification of TBX2 as a target for PAX3 provides a key insight into how PAX3 may contribute to melanoma evolution and may provide opportunities for prosenescence therapeutic intervention aimed at disrupting the ability of PAX3 to regulate TBX2.


Subject(s)
Cell Lineage , Gene Expression Regulation , Melanocytes/metabolism , Paired Box Transcription Factors/metabolism , T-Box Domain Proteins/genetics , Animals , Cell Lineage/genetics , Cell Proliferation , Cell Survival/genetics , Chromatin Immunoprecipitation , Gene Expression Regulation, Neoplastic , Humans , Melanocytes/pathology , Melanoma/genetics , Melanoma/pathology , Mice , Oligonucleotide Array Sequence Analysis , PAX3 Transcription Factor , Promoter Regions, Genetic/genetics , Protein Binding/genetics , T-Box Domain Proteins/metabolism , Up-Regulation/genetics
20.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 28(8): 801-3, 2012 Aug.
Article in Chinese | MEDLINE | ID: mdl-22863583

ABSTRACT

AIM: To establish a hepatocellular carcinoma model of BALB/c mouse and to study the expression and potential role of metastasis-associated protein 1 (MTA1) in the carcinogenesis process. METHODS: Normal adult male BALB/c mice were induced by the combined dimethylnitrosamine (DEN)/carbon tetrachloride (CCl(4);)/alcohol for 150 d. The morphological changes in liver cells and the expression of MTA1 in the liver lesions were observed by HE and immunohistochemical stainings, respectively. RESULTS: The pathological changes of the survivals' livers in experimental group were liver inflammation, fibrosis and cancer in sequence. The level of MTA1 increased in the carcinogenesis process, and MTA1 was mainly expressed in the cytoplasm of the cells suffering cirrhosis. CONCLUSION: The changes of the expression sites and quantity of MTA1 in the DEN-induced carcinogenesis of mouse liver indicate that MTA1 may play an important role in the whole process of liver carcinogenesis.


Subject(s)
Carcinoma, Hepatocellular/genetics , Cell Transformation, Neoplastic/genetics , Gene Expression , Liver Neoplasms/genetics , Transcription Factors/genetics , Animals , Carcinoma, Hepatocellular/metabolism , Cell Transformation, Neoplastic/metabolism , Disease Models, Animal , Liver Neoplasms/metabolism , Liver Neoplasms/pathology , Male , Mice , Mice, Inbred BALB C , Repressor Proteins , Trans-Activators , Transcription Factors/metabolism
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