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1.
Zhongguo Zhong Yao Za Zhi ; 45(1): 124-132, 2020 Jan.
Article in Chinese | MEDLINE | ID: mdl-32237421

ABSTRACT

The chemical constituents of Cinnamomi Ramulus were investigated in this study. Twenty-two compounds were isolated by silica gel, Sephadex LH-20 gel column chromatographies and preparative HPLC and their structures were identified by various spectral analyses as dihydrorosavin(1), rosavin(2), 1-phenyl-propane-1,2,3-triol(3), patchoulol(4), graphostromane B(5),(+)-lyoniresinol-3 a-O-ß-D-glucopyranoside(6),(-)-lyoniresinol-3 a-O-ß-D-glucopyranoside(7), cinnacaside(8), subaveniumin A(9), 3-phenyl-2-propenyl-6-O-L-arabinopyranosyl-ß-glucopyranoside(10), 2-phenylethyl-ß-vicianoside(11), cinnacasol(12), [(2R,3S,4S,5R,6R)-6-(benzyloxy)-3,4,5-trihydroxytetrahydro-2H-pyran-2-yl] methyl hydrogen sulfate(13), coniferyl aldehyde(14),(2R,3R)-5,7-dimethoxy-3',4'-methylenedioxyflavan-3-ol(15), cinnacassin L(16), E-cinnamic alcohol(17),(E)-3-(2-methoxyphenyl)-2-propen-1-ol(18), 2-hydroxyphenylpropanol(19), cinnamomulactone(20),(+)-syringaresinol(21) and cinnamomumolide(22), respectively. Among them, 1 is a new compound and 3-7, 9-11, 13, 15, 18 and 19 were isolated from the plant for the first time.


Subject(s)
Cinnamomum/chemistry , Phytochemicals/analysis , Chromatography, High Pressure Liquid , Drugs, Chinese Herbal
2.
Chin J Nat Med ; 15(9): 641-652, 2017 Sep.
Article in English | MEDLINE | ID: mdl-28991525

ABSTRACT

Alzheimer's disease (AD) is a neurodegenerative disorder characterized by the loss of patients' memory and their cognitive abilities and the mechanism is not completely clear. Although a variety of drugs have been approved for the AD treatment, substances which can prevent and cure AD are still in great need. The effect of polysaccharides from traditional Chinese medicine (TCM) on anti-AD has gained great progress and attained more and more attention in recent years. In this review, research advances in TCM-polysaccharides on AD made in this decade are summarized.


Subject(s)
Alzheimer Disease/drug therapy , Drugs, Chinese Herbal/administration & dosage , Plants, Medicinal/chemistry , Polysaccharides/administration & dosage , Animals , Humans , Medicine, Chinese Traditional
3.
Molecules ; 21(6)2016 Jun 15.
Article in English | MEDLINE | ID: mdl-27314319

ABSTRACT

A neutral α-glucan, named BP1, with a molecular mass of approximately 9.45 kDa, was isolated from Lobelia chinensis by hot-water extraction, a Q-Sepharose Fast Flow column and Superdex-75 column chromatography. Its chemical structure was characterized by monosaccharide analysis, methylation analysis and analysis of its FT-IR, high performance gel permeation chromatography (HPGPC) and 1D/2D-NMR spectra data. The backbone of BP1 consists of →6α-d-Glcp¹â†’6,3α-d-Glcp¹â†’(6α-d-Glcp¹)x-6,3α-d-Glcp¹-(6α-d-Glcp¹)y→. The side chains were terminal α-d-Glcp¹â†’ and α-d-Glcp¹â†’ (6α-d-Glcp¹)z→4α-d-Glcp¹â†’3α-d-Glcp¹â†’4α-d-Glcp¹â†’ (x + y + z = 5), which are attached to the backbone at O-3 of 3,6α-d-Glcp¹. The results of the effect of BP1 on mouse macrophage cell line RAW 264.7 indicate that BP1 enhances the cell proliferation, phagocytosis, nitric oxide production and cytokine secretion in a dose-dependent manner. Because the inhibitor of Toll-like receptor 4 blocks the BP1-induced secretion of TNF-α and IL-6, we hypothesize that α-glucan BP1 activates TLR4, which mediates the above-mentioned immunomodulating effects.


Subject(s)
Glucans/chemistry , Interleukin-6/biosynthesis , Lobelia/chemistry , Toll-Like Receptor 4/biosynthesis , Tumor Necrosis Factor-alpha/biosynthesis , Animals , Gene Expression Regulation/drug effects , Glucans/administration & dosage , Glucans/isolation & purification , Immunomodulation , Methylation , Mice , Monosaccharides/chemistry , Monosaccharides/isolation & purification , Phagocytosis/drug effects , RAW 264.7 Cells/drug effects , Spectroscopy, Fourier Transform Infrared
4.
Chin J Integr Med ; 21(3): 196-203, 2015 Mar.
Article in English | MEDLINE | ID: mdl-23860800

ABSTRACT

OBJECTIVE: Although chondroprotective activities have been documented for polysaccharides, the potential target of different polysaccharide may differ. The study was aimed to explore the effect of glucan HBP-A in chondrocyte monolayer culture and chondrocytes-alginate hydrogel constructs in vivo, especially on the expression of type II collagen. METHODS: Chondrocytes isolated from rabbit articular cartilage were cultured and verified by immunocytochemical staining of type II collagen. Chondrocyte viability was assessed after being treated with HBP-A in different concentrations. Morphological status of chondrocytes-alginate hydrogel constructs in vitro was observed by scanning electron microscope (SEM). The constructs were treated with HBP-A and then injected to nude mice subcutaneously. Six weeks after transplantation, the specimens were observed through transmission electron microscopy (TEM). The mRNA expressions of disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTs-5), aggrecan and type II collagen in both monolayer culture and constructs were determined by real time polymerase chain reaction (PCR). The expression of type II collagen and matrix metalloproteinases-3 (MMP-3) in chondrocyte monolayer culture was also tested through Western blot and enzyme linked immunosorbent assay (ELISA), respectively. RESULTS: MMP-3 secretion and ADAMTs-5 mRNA expression in vitro were inhibited by HBP-A at 0.3 mg/mL concentration. In morphological study, there were significant appearance of collagen in those constructs treated by HBP-A. Accordingly, in both chondrocyte monolayer culture and chondrocytes-alginate hydrogel constructs, the expression of type II collagen was increased significantly in HBP-A group when compared with control group (P<0.001). CONCLUSIONS: The study documented that the potential pharmacological target of glucan HBP-A in chondrocytes monolayer culture and tissue engineered cartilage in vivo may be concerned with the inhibition of catabolic enzymes MMP-3, ADAMTs-5, and increasing of type II collagen expression.


Subject(s)
Cartilage, Articular/physiology , Chondrocytes/metabolism , Collagen Type II/metabolism , Glucans/pharmacology , Tissue Engineering/methods , ADAM Proteins/genetics , ADAM Proteins/metabolism , Aggrecans/genetics , Aggrecans/metabolism , Alginates/pharmacology , Animals , Cartilage, Articular/drug effects , Cell Proliferation/drug effects , Cell Shape/drug effects , Cell Survival/drug effects , Chondrocytes/cytology , Chondrocytes/drug effects , Chondrocytes/ultrastructure , Collagen Type II/genetics , Female , Glucuronic Acid/pharmacology , Hexuronic Acids/pharmacology , Hydrogel, Polyethylene Glycol Dimethacrylate/pharmacology , Immunohistochemistry , Matrix Metalloproteinase 3/metabolism , Mice, Nude , RNA, Messenger/genetics , RNA, Messenger/metabolism , Rabbits
5.
Yao Xue Xue Bao ; 48(8): 1253-8, 2013 Aug.
Article in Chinese | MEDLINE | ID: mdl-24187832

ABSTRACT

The present study was designed to observe the protection of Grateloupia filicina polysaccharide (GFP) against hepatotoxicity induced by Dioscorea bulbifera L in mice and its underlying mechanism. GFP was intragastrically (ig) given to mice at various doses. After 6 days, the mice were treated with ethyl acetate extract of Dioscorea bulbifera L (EF, ig). Serum levels of alanine/aspartate aminotransferase (ALT/AST), alkaline phosphatase (ALP), total bilirubin (TB) were measured, and liver histological evaluation was conducted. Furthermore, reductions of liver glutathione (GSH) amount and glutamate cysteine ligase (GCL) activity were tested. The expressions of GCL-c, GCL-m, and HO-1 (heme oxygenase-1) in liver were observed by Western-blot. The results showed that GFP (600 mg x kg(-1)) decreased EF-induced the increase of serum ALT, AST and TB, and GFP (400, 600 mg x kg(-1)) inhibited EF-induced the increase of serum ALP. Liver histological evaluation showed that the liver injury induced by EF was relieved after treated with GFP. GFP further increased liver GSH amount and reversed EF-induced the decrease of GCL activity. The Western-blot result showed that GFP augmented EF-induced the increase of HO-1, and reversed EF-induced the decrease of GCL-c. In conclusion, GFP can act against the oxidative stress liver injury induced by Dioscorea bulbifera L in mice.


Subject(s)
Chemical and Drug Induced Liver Injury/metabolism , Dioscorea/toxicity , Heterocyclic Compounds, 4 or More Rings/toxicity , Polysaccharides/pharmacology , Rhodophyta/chemistry , Alanine Transaminase/blood , Alkaline Phosphatase/blood , Animals , Aspartate Aminotransferases/blood , Bilirubin/blood , Chemical and Drug Induced Liver Injury/blood , Glutamate-Cysteine Ligase/metabolism , Glutathione/metabolism , Heme Oxygenase-1/metabolism , Heterocyclic Compounds, 4 or More Rings/antagonists & inhibitors , Heterocyclic Compounds, 4 or More Rings/isolation & purification , Liver/metabolism , Liver/pathology , Male , Mice , Mice, Inbred ICR , Oxidative Stress/drug effects , Plants, Medicinal/chemistry , Polysaccharides/isolation & purification , Random Allocation
6.
BMC Complement Altern Med ; 13: 227, 2013 Sep 17.
Article in English | MEDLINE | ID: mdl-24041039

ABSTRACT

BACKGROUND: In Traditional Chinese Medicine (TCM), tongue diagnosis has been an important diagnostic method for the last 3000 years. Tongue diagnosis is a non-invasive, simple and valuable diagnostic tool. TCM treats the tongue coating on a very sensitive scale that reflects physiological and pathological changes in the organs, especially the spleen and stomach. Tongue coating can diagnose disease severity and determine the TCM syndrome ("Zheng" in Chinese). The biological bases of different tongue coating appearances are still poorly understood and lack systematic investigation at the molecular level. METHODS: Tongue coating samples were collected from 70 chronic gastritis patients and 20 normal controls. 16S rRNA denatured gradient gel electrophoresis (16S rRNA-DGGE) and liquid chromatography and mass spectrometry (LC-MS) were designed to profile tongue coatings. The statistical techniques used were principal component analysis and partial least squares-discriminate analysis. RESULTS: Ten potential metabolites or markers were found in chronic gastritis patients, including UDP-D-galactose, 3-ketolactose, and vitamin D2, based on LC-MS. Eight significantly different strips were observed in samples from chronic gastritis patients based on 16S rRNA-DGGE. Two strips, Strips 8 and 10, were selected for gene sequencing. Strip 10 sequencing showed a 100% similarity to Rothia mucilaginosa. Strip 8 sequencing showed a 96.2% similarity to Moraxella catarrhalis. CONCLUSIONS: Changes in glucose metabolism could possibly form the basis of tongue coating conformation in chronic gastritis patients. The study revealed important connections between metabolic components, microecological components and tongue coating in chronic gastritis patients. Compared with other diagnostic regimens, such as blood tests or tissue biopsies, tongue coating is more amenable to, and more convenient for, both patients and doctors.


Subject(s)
Gastritis/metabolism , Gastritis/microbiology , Tongue/metabolism , Tongue/microbiology , Adult , Case-Control Studies , DNA, Bacterial/analysis , DNA, Bacterial/genetics , DNA, Bacterial/isolation & purification , Denaturing Gradient Gel Electrophoresis , Female , Humans , Least-Squares Analysis , Male , Metabolome , Middle Aged , RNA, Ribosomal, 16S , Tongue/chemistry
7.
Article in English | MEDLINE | ID: mdl-22778781

ABSTRACT

Metabolic profiling is widely used as a probe in diagnosing diseases. In this study, the metabolic profiling of urinary carbohydrates was investigated using gas chromatography/mass spectrometry (GC/MS) and multivariate statistical analysis. The kernel-based orthogonal projections to latent structures (K-OPLS) model were established and validated to distinguish between subjects with and without diabetes mellitus (DM). The model was combined with subwindow permutation analysis (SPA) in order to extract novel biomarker information. Furthermore, the K-OPLS model visually represented the alterations in urinary carbohydrate profiles of excess and deficiency syndromes in patients with diabetes. The combination of GC/MS and K-OPLS/SPA analysis allowed the urinary carbohydrate metabolic characterization of DM patients with different traditional Chinese medicine (TCM) syndromes, including biomarkers different from non-DM patients. The method presented in this study might be a complement or an alternative to TCM syndrome research.

8.
Yao Xue Xue Bao ; 47(10): 1358-62, 2012 Oct.
Article in English | MEDLINE | ID: mdl-23289149

ABSTRACT

A new oleanane-type triterpenoid glycoside, 3beta-O-(f-D-xylopyranosyl-(1-->2)-[beta-D-glucopyranosyl (1-->4)-beta-D-glucopyranosyl-(1 -->3)]-beta-D-fucopyranosyl-11, 13(18) diene-olean-23alpha, 28 diol (1), along with three known phenylethyl glycosides (2-4), was isolated from the root of traditional Chinese medicine Scrophularia ningpoensis. Among them, compounds 2 and 3 were obtained from Scrophularia genus for the first time. The structure of compound 1 was elucidated on the basis of spectroscopic method including 1D, 2D NMR and HR-ESI-MS.


Subject(s)
Drugs, Chinese Herbal/isolation & purification , Glycosides/isolation & purification , Scrophularia/chemistry , Triterpenes/isolation & purification , Drugs, Chinese Herbal/chemistry , Glycosides/chemistry , Molecular Structure , Plant Roots/chemistry , Plants, Medicinal/chemistry , Triterpenes/chemistry
9.
Zhong Yao Cai ; 33(5): 726-9, 2010 May.
Article in Chinese | MEDLINE | ID: mdl-20873556

ABSTRACT

OBJECTIVE: To study a glucan (GB II) isolated from Gastrodia elata. METHODS: The glucan was obtained with water extraction, alcohol precipitate, DEAE-Sepharose Fast Flow column and Sepharose CL-6B column chromatography; sugar composition analysis, IR and NMR were used to determine the structural feature. RESULTS: The molecular weight of the glucan was 4 300 dalton estimated by HPGPC; it contained 27 glucose residues, which mainly composed of alpha-D-(1-->4)-glucose with little glucuronic acid and branch O-6 points. CONCLUSION: The glucan was a new glucan for the first report from Gastrodia elata.


Subject(s)
Gastrodia/chemistry , Glucans/chemistry , Glucuronic Acid/analysis , Monosaccharides/analysis , Plants, Medicinal/chemistry , Gas Chromatography-Mass Spectrometry , Glucans/isolation & purification , Glucuronic Acid/isolation & purification , Methylation , Molecular Structure , Molecular Weight , Monosaccharides/chemistry , Monosaccharides/isolation & purification , Plant Tubers/chemistry , Spectrophotometry, Infrared
10.
J Nat Prod ; 72(11): 1964-8, 2009 Nov.
Article in English | MEDLINE | ID: mdl-19842682

ABSTRACT

Four new steroidal sapogenins (1-4), named diosbulbisins A-D, two new spirostane glycosides, diosbulbisides A (5) and B (6), one new cholestane glycoside, diosbulbiside C (7), and the known compounds 8-10 were isolated from rhizomes of Dioscorea bulbifera. Their structures were elucidated by 1D and 2D NMR techniques, HRFTMS, and chemical methods. The unusual furospirostanol sapogenin skeletons, as found in compounds 3 and 4, are reported in the family Dioscoreaceae for the first time. Cytotoxicity of compounds 1-10 was evaluated using two human hepatocellular carcinoma cell lines (Bel-7402 and SMMC7721).


Subject(s)
Antineoplastic Agents, Phytogenic/isolation & purification , Dioscorea/chemistry , Drugs, Chinese Herbal/isolation & purification , Glycosides/isolation & purification , Sapogenins/isolation & purification , Steroids/isolation & purification , Antineoplastic Agents, Phytogenic/chemistry , Antineoplastic Agents, Phytogenic/pharmacology , Drug Screening Assays, Antitumor , Drugs, Chinese Herbal/chemistry , Drugs, Chinese Herbal/pharmacology , Glycosides/chemistry , Glycosides/pharmacology , Humans , Molecular Structure , Nuclear Magnetic Resonance, Biomolecular , Rhizome/chemistry , Sapogenins/chemistry , Sapogenins/pharmacology , Steroids/chemistry , Steroids/pharmacology
11.
J Agric Food Chem ; 56(8): 2624-9, 2008 Apr 23.
Article in English | MEDLINE | ID: mdl-18376844

ABSTRACT

China is a major producer of oats; the annual harvested area of 350,000 ha yields approximately 465,000 tons, giving an average yield of 1.33 tons/ha. The bran is not used for animal feed as it is of poor digestibility and low nutritive content and is considered a waste byproduct. Therefore, it is advantageous to produce a value-added product from the bran. We extracted the native polysaccharide, a linear (1-3)-, (1-4)-linked beta-glucan (OBG) from the oat bran and synthesized a sulfated derivative OBGS containing 36.5% sulfate. OBGS had potent activity against a primary isolate of human immunodeficiency virus (HIV-1) in peripheral blood mononuclear cells at a concentration (EC(50)=5.98 x 10(-4) microM) approximately 15,000 times below its cytotoxic concentration. OBGS was also active postinfection (EC(50)=5.3 x 10(-4) microM) and protected pretreated peripheral mononuclear cells (EC(50)=5.2 x 10(-2) microM) washed free of the compounds prior to infection. Thus, OBGS has potential as a vaginal microbicide and is the first such report for oat bran derived sulfated beta-glucan.


Subject(s)
Anti-HIV Agents/chemical synthesis , Avena/chemistry , Polysaccharides/chemical synthesis , Sulfates/chemical synthesis , beta-Glucans/chemistry , Anti-HIV Agents/pharmacology , HIV-1/drug effects , Humans , Leukocytes, Mononuclear/virology , Polysaccharides/pharmacology , Seeds/chemistry , Sulfates/pharmacology
12.
Zhongguo Zhong Yao Za Zhi ; 32(18): 1893-5, 2007 Sep.
Article in Chinese | MEDLINE | ID: mdl-18051899

ABSTRACT

OBJECTIVE: To study the chemical constituents of root of Actinidia macrosperma. METHOD: Chromatographic methods were used to isolate compounds from A. macrosperma and spectroscopic methods were used to identify the structures of the isolated compounds. RESULT: Eight compounds were obtained and identified as 12-oleanene-2alpha, 3alpha, 24-triol (1), isotachioside (2), asiatic acid (3), catechin (4), epicatechin (5), ursolic acid (6), beta-daucosterol (7), beta-sitosterol (8). CONCLUSION: All these compounds were isolated from this plant for the first time, compound 1, 2 were obtained from this genus for the first time.


Subject(s)
Actinidia/chemistry , Catechin/isolation & purification , Glucosides/isolation & purification , Hydroquinones/isolation & purification , Oleanolic Acid/analogs & derivatives , Plant Roots/chemistry , Triterpenes/isolation & purification , Catechin/chemistry , Glucosides/chemistry , Hydroquinones/chemistry , Oleanolic Acid/chemistry , Oleanolic Acid/isolation & purification , Pentacyclic Triterpenes , Plants, Medicinal/chemistry , Triterpenes/chemistry
13.
Zhongguo Zhong Yao Za Zhi ; 31(13): 1075-7, 2006 Jul.
Article in Chinese | MEDLINE | ID: mdl-17048606

ABSTRACT

OBJECTIVE: To study the polysaccharide of Salvia miltiorrhiza. METHOD: The root was extracted by water and purified preliminarily by alcohol precipitation. The obtained crude polysaccharide purified by ion exchange resin DEAE-Sepharose Fast Flow then bleached with H2O2 and dialysed. Two portions of light yellow powder were obtained through lyophilization and named as SMP 1, SMP 0.5. On the basis of 13C-NMR, DEPT, IR spectra, monosaccharide composite analysis and part monosaccharide composite analysis, their structures were identified. RESULT: The molecular weight of SMP 1, SMP 0.5 was estimated to be 1.39 x 10(6), 4.03 x 10(5), respectively, and the structure of SMP 1 was mainly alpha-(1-->6) D-Glc linked a few alpha-(1-->2) D-Glc, SMP 0.5 was mainly a alpha-(1-->6) D-Glucosan. CONCLUSION: SMP 1 and SMP0.5 were neutral homosaccharides that extracted from S. miltiorrhiza for the first time.


Subject(s)
Plants, Medicinal/chemistry , Polysaccharides/chemistry , Salvia miltiorrhiza/chemistry , Molecular Weight , Plant Roots/chemistry , Polysaccharides/isolation & purification
14.
Br J Pharmacol ; 148(6): 741-51, 2006 Jul.
Article in English | MEDLINE | ID: mdl-16715123

ABSTRACT

1. The antiangiogenic and antitumor properties of Grateloupia longifolia polysaccharide (GLP), a new type of polysaccharide isolated from the marine alga, were investigated with several in vitro and in vivo models. Possible mechanisms underlying its antiangiogenic activity were also assessed. 2. GLP dose-dependently inhibited proliferation of human microvascular endothelial cells (HMEC-1) and human umbilical vein endothelial cells (HUVEC), with IC50 values of 0.86 and 0.64 mg ml(-1), respectively. In tube formation and cell migration assays using HMEC-1 cells, noncytotoxic doses of GLP significantly inhibited formation of intact tube networks and reduced the number of migratory cells. Inhibition by GLP was VEGF-independent. 3. In the chick chorioallantoic membrane (CAM) assay, GLP (2.5 microg egg(-1)) reduced new vessel formation compared with the vehicle control. GLP (0.1 mg plug(-1)) also reduced the vessel density in Matrigel plugs implanted in mice. 4. The levels of pan and phosphorylated receptors for VEGF, VEGFR-1 (flt-1) and VEGFR-2 (KDR) were not significantly altered by 5 mg ml(-1) GLP treatment of HMEC-1, although tissue factor (TF) showed significant decreases at both mRNA and protein levels following GLP treatment. 5. In mice bearing sarcoma-180 cells, intravenous administration of GLP (200 mg kg(-1)) decreased tumor weight by 52% without obvious toxicity. Vascular density in sections of the tumor was reduced by 64% after GLP treatment. 6. Collectively, these results indicate that GLP has antitumor properties, associated at least, in part, with the antiangiogenesis induced by downregulation of TF.


Subject(s)
Angiogenesis Inhibitors/pharmacology , Endothelial Cells/drug effects , Eukaryota/chemistry , Gene Expression Regulation/drug effects , Polysaccharides/pharmacology , Thromboplastin/genetics , Animals , Cell Line , Cell Movement/drug effects , Cell Proliferation/drug effects , Down-Regulation , Endothelial Cells/metabolism , Fibroblast Growth Factor 2/antagonists & inhibitors , Humans , Mice , Mice, Inbred C57BL , Neovascularization, Pathologic/prevention & control , Sarcoma 180/blood supply , Sarcoma 180/drug therapy , Vascular Endothelial Growth Factor A/physiology
15.
Zhongguo Zhong Yao Za Zhi ; 31(20): 1661-4, 2006 Oct.
Article in Chinese | MEDLINE | ID: mdl-17225527

ABSTRACT

The collected information is an attempt to cover the more recent developments in the phytochemistry and pharmacology of this genus. During the past years, alkaloids, flavonoids, volatile oils, organic acids, polysaccharides, tannins and phenolic constituents have been isolated from Ephedra. Pharmacological studies are described according to hypoglycemic effects, anticoagulated blood properties, depressurization, immunosuppressive activity, antioxidation and antivirus activity and so on. The information summarized here is intended to provid a rational foundation for the futher development and utilization of Ephedra which is rich in China.


Subject(s)
Ephedra/chemistry , Hypoglycemic Agents/pharmacology , Immunosuppressive Agents/pharmacology , Plants, Medicinal/chemistry , Polysaccharides/isolation & purification , Alkaloids/chemistry , Alkaloids/isolation & purification , Animals , Ephedra/classification , Flavonoids/chemistry , Flavonoids/isolation & purification , Molecular Structure , Plant Stems/chemistry , Polysaccharides/pharmacology
16.
Zhong Yao Cai ; 29(9): 912-5, 2006 Sep.
Article in English | MEDLINE | ID: mdl-17212045

ABSTRACT

OBJECTIVE: To isolate a homogeneous polysaccharide RP from RDP (a crude polysaccharide from Dioscorea opposita Thunb.), and study its preliminary com-position and structure. METHODS: RP was obtained with water extraction, alcohol sedimentation, CTAB deprotein, cellulose column and SephadexG-100 column. The purities of RP were identified by SephadexG-200. PC analysis on its acidic hydrolysates was used to determine the sugar components. Sephadex chromatography was used to mensurate its molecular weight. IR was used to analysis RP. RESULTS: RP was homogeneous. IR indicated that RP had P configurations. The compositon was identified by paper chromatography as glucose, D-mannose, D-galactose. CONCLUSION: The research could provide a theoretical foundation for further development and utilization.


Subject(s)
Glucans/chemistry , Glucans/isolation & purification , Hedyotis/chemistry , Plants, Medicinal/chemistry , Glucose/chemistry , Molecular Structure , Molecular Weight , Spectrometry, Mass, Electrospray Ionization
17.
Zhongguo Zhong Yao Za Zhi ; 27(2): 128-30, 2002 Feb.
Article in Chinese | MEDLINE | ID: mdl-12774387

ABSTRACT

OBJECTIVE: To study the chemical features of CPB-4, a heteropolysaccharide obtained from Cynanchum paniculatum. METHOD: Sugar composition analysis, methylation analysis, partial hydrolysis and carbon-13 nuclear magnetic resonance were used to determine the sugar composition, linkages, main chain, branch chains and branching points. RESULT: CPB-4 is composed of L-arabinose, L-xylose, L-rhamnose and D-galactose in closely molar ratios of 0.8:0.2:0.2:1.0. Its main chain is comprised of 1, 5 linked galactose and side chains are comprised of terminal xylose, terminal arabinose, oligosaccharide of arabinose and oligosaccharide of arabinose, rhamnose and galactose. The branching points are located at C-6 and C-2 of galactose. CONCLUSION: CPB-4 is a new heteropolysaccharide from C. paniculatum.


Subject(s)
Cynanchum/chemistry , Plants, Medicinal/chemistry , Polysaccharides/chemistry , Arabinose/isolation & purification , Methylation , Plant Roots/chemistry , Polysaccharides/isolation & purification , Rhamnose/isolation & purification , Xylose/isolation & purification
18.
Yao Xue Xue Bao ; 37(7): 497-500, 2002 Jul.
Article in Chinese | MEDLINE | ID: mdl-12914316

ABSTRACT

AIM: To study the effect of Angelica sinsensis polysaccharides on lymphocyte proliferation and induction of IFN-gamma. METHODS: Angelica sinensis polysaccharides(AP) were separated into AP-I, AP-II, AP-III and AP-IV by alcohol deposition with different concentration. The radioactivities of [3H]-TdR uptake by lymphocyte were used to determine the ability of lymphocyte. The bioactivity of IFN-gamma was measured by violet crystalline dying. RESULTS: AP-IV was found to be composed of Ara and Glu in the ratio of 0.99:6.47, the molecular weight was estimated to be 5,600. AP-I and AP-II 100 mg.kg-1 i.p. were found to significantly augment mice splenocyte proliferation, release IFN-gamma and increase IFN-gamma bioactivity. 50 micrograms.mL-1 AP-I, AP-II and AP-III were shown to enhance the proliferative response of the mouse spleen lymphocytes in vitro. CONCLUSION: AP-I and AP-II showed higher immunoactivity than AP-III, AP-IV had no effect.


Subject(s)
Angelica sinensis/chemistry , Drugs, Chinese Herbal/pharmacology , Interferon-gamma/biosynthesis , Lymphocytes/drug effects , Polysaccharides/pharmacology , Animals , Cell Division/drug effects , Drugs, Chinese Herbal/isolation & purification , Immunity/drug effects , Lymphocytes/cytology , Lymphocytes/immunology , Male , Mice , Mice, Inbred BALB C , Plants, Medicinal/chemistry , Polysaccharides/isolation & purification , Random Allocation , Spleen/cytology , Tumor Cells, Cultured
19.
Article in English | MEDLINE | ID: mdl-12075425

ABSTRACT

A polysaccharide was purified from a traditional Chinese drug, "Xu-Chang-Qing" (Cynanchum panilatum (Bunge) Kitagawa), and its molecular weight was estimated to be 1.5x10(4) by HPLC method. Its specific rotation [alpha](D) was 151.4 deg; (0.96, H(2)O). Sugar composition analysis indicated this polysaccharide was composed only of glucose. Methylation analysis, partially hydrolysis, acetolysis, IR and NMR data showed that its main chain was composed of alpha-D 1,4 linked glucosyl residues and its side chains were composed of alpha-D 1,4 and alpha-D 1,6 linked glucosyl residues. There was one branch located at O-6 of the glucosyl residue in every repeating unit containing five-glucosyl residues.

20.
Article in English | MEDLINE | ID: mdl-12114978

ABSTRACT

A series of sulfated derivatives of lentinan were prepared by the modified Wolfrom method. Sulfates content analysis indicated that the proportion of sulfate substitution depended on the reaction time and the ratio of chlorsulfonic acid and pyridine in the ester reagent. Methylation analysis by Needs method and Hakomori method was proved not to be suitable for the structural analysis of sulfated lentinan. (13)C-NMR data showed that sulfates were located on the carbon-6 in all sulfated derivatives of lentinan showing the higher reactivity of the hydroxyls on C-6 position than those at other position.

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