ABSTRACT
Melamine is commonly used in the chemical industry, and it has been found to exist on food processing equipment and utensils. Previous investigations suggested that melamine alone or its combination with cyanuric acid appears to be toxic to immune system in animals. The objective of this study is to investigate the potential effects of melamine with or without cyanuric acid on immune function in ovalbumin- sensitized mice. Our data indicated that melamine-related administration caused a significant decreasing in the content of IL-4 in mice in comparison to negative control group. Significant increasing in the content of histamine (HIS) was recorded in almost all treated groups. The co-administration of melamine and cyanuric acid with high dose (each at 16mgkg-12d-1) led to a significantly lower contents of IL-10, IL-16 and monocyte chemoattractant protein-1 (MCP-1) in mouse serum in comparison to negative control group. Moreover, our data indicated that the number of Th1 and Th2 cells, and the ratio of Th1/Th2 spleen lymphocytes were significantly changed after treatment. Also, our results demonstrated that the profiles of both CD40 and CD40L were significantly altered in spleen lymphocytes after treatment.
Subject(s)
Environmental Pollutants/toxicity , Immunity, Innate , Triazines/toxicity , Animals , Male , Mice , Ovalbumin/immunology , Toxicity TestsABSTRACT
Melamine is an industrial chemical with high nitrogen content. When added to the pet food and milk it can falsely elevate the apparent protein concentration readings. Cyanuric acid related structurally to melamine has a strong mutual affinity with melamine. The combined ingestion of melamine and cyanuric acid was considered to be responsible for the crystalluria, kidney stones and subsequent renal failure in animals. In our previous investigation, we demonstrated that melamine alone or its combination with cyanuric acid appears to be toxic to the immune system in mice. The objective of this study was to investigate the potential effects of melamine on humoral immunity with or without cyanuric acid in mice. In comparison to control group, a significantly lower content of plasma cells expressing CD138 were observed in mixture groups of melamine and cyanuric acid with both middle and high doses. The co-administration of melamine and cyanuric acid resulted in a significant decreasing in blimp-1 protein expression and the contents of sIgA, C3, IL-21 and IL-4 compared with the control group. Moreover, our data clearly showed that melamine-related toxicity suppressed the production of IL-6 and IL-10 in a dose-dependent manner. Also, the animals from mixture of melamine and cyanuric acid with high dose group exhibited a significantly lower expression of gata-3 protein, The results from the present study suggested that the exposure to melamine alone or combination with cyanuric acid had certain humoral immunotoxicity in mice, especially when ingested in high dosage.
Subject(s)
Immunity, Humoral/drug effects , Interleukins/metabolism , Triazines/toxicity , Animals , Dose-Response Relationship, Drug , Drug Synergism , GATA3 Transcription Factor/genetics , GATA3 Transcription Factor/metabolism , Gene Expression Regulation/drug effects , Gene Expression Regulation/immunology , Immunoglobulin A, Secretory/genetics , Immunoglobulin A, Secretory/metabolism , Interleukins/genetics , Mice , Positive Regulatory Domain I-Binding Factor 1 , Renal Insufficiency , Syndecan-1/metabolism , Transcription Factors/genetics , Transcription Factors/metabolism , Triazines/pharmacokineticsABSTRACT
Melamine is an organic nitrogenous compound whose acute toxicity was generally thought to be low in animals. In the present work, we investigated the potential cytotoxic effects of melamine on spleen lymphocytes in mice. In the treated group, morphological changes were observed in cultured lymphocytes in vitro. The co-administration of melamine and cyanuric acid caused a declining tendency in stimulation index of spleen lymphocyte. All treated groups had lower ratios of CD4+/CD8+. Both early apoptotic and late apoptotic/necrotic rates of lymphocyte were significantly higher in the co-administration high groups of melamine and cyanuric acid. Melamine-related toxicity promoted the expression of Bax mRNA, and suppressed the expression of Bcl-2 mRNA in spleen of the treated mice. These results provided useful information for assessing the toxicity of melamine on immune system of mammals, and contributed to the existing toxic profile of melamine.
Subject(s)
Environmental Pollutants/toxicity , Lymphocytes/drug effects , Resins, Synthetic/toxicity , Spleen/drug effects , Triazines/toxicity , Animals , Gene Expression Regulation/drug effects , Male , Mice , Spleen/physiopathologyABSTRACT
This paper provides the details of a multiaxis electric field sensor. The sensing element consists of three slab coupled optical-fiber sensors that are combined to allow directional electric field sensing. The packaged three-axis sensor has a small cross-sectional area of 0.5 cm×0.5 cm by using an x-cut crystal. A method is described that uses a sensitivity-matrix approach to map the measurements to field components. The calibration and testing are described, resulting in an average error of 1.5°.
ABSTRACT
This paper provides a detailed analysis of electric field sensing using a slab-coupled optical fiber sensor (SCOS). This analysis explains that the best material for the slab waveguide is an inorganic material because of the low RF permittivity combined with the high electro-optic coefficient. The paper also describes the fabrication and testing of a SCOS using an AJL chromophore in amorphous polycarbonate. The high uniform polymer slab waveguide is fabricated using a hot embossing process to create a slab with a thickness of 50 µm. The fabricated polymer SCOS was characterized to have a resonance slope of ΔP/Δλ=6.83E5 W/m and a resonance shift of Δλ/E=1.47E-16 m(2)/V.
