Citizen science in action: Time-resolved immunofluorescence-based field detection of antibiotics with portable analytical kit.

Citizen scientist-based environmental monitoring and public education are becoming increasingly popular. However, current technologies for antibiotic-based novel contaminant identification are still restricted to laboratory sample collection and analysis due to detection methodologies and apparatus limitations. This study developed a time-resolved immunofluorescence-based simultaneous field-based assay for ciprofloxacin (CIP) and enrofloxacin (ENR) that matches test results to geographic locations. The assay helps the public understand the potential levels of antibiotic exposures in their environments and helps them take appropriate action to reduce risk. The assay was developed using smartphones and social software in addition to rapid testing. The method uses a portable, low-cost analytical kit with a smartphone app to build a field-based detection platform for the detection and analysis of ENR and CIP in water and aquatic products. The methodological evaluation was good, with detection limits of 0.4 ng/mL and 0.5 ng/g for ENR in water and fish, and quantification limits of 1.2 ng/mL and 1.4 ng/g, with recoveries of 89.0 %-101.0 % and 78.0 %-97.0 %. For CIP in water and fish, the limits of detection were 0.3 ng/mL and 0.4 ng/g, the limits of quantification were 0.9 ng/mL and 1.2 ng/g, and the recoveries were 75.0 %-91.0 % and 72.0 %-89.0 %, both with coefficients of variation <15 %. These limits were sufficient to prevent the two antibiotics from crossing over during simultaneous detection. The assay was validated using real samples to assess the effectiveness of the assay platform in field deployments, and the results were consistent with those obtained through liquid chromatography-tandem mass spectrometry (LC-MS) and enzyme-linked immunoassay (ELISA) techniques. In addition, the TRFIA assay process requires less time, uses more portable instruments, and is less complex than traditional methods. This study provides a new scientific, accurate, and rapid detection method for antibiotic detection by citizen scientists, helping scientists to obtain a wider range of data and providing more opportunities to solve scientific problems.

Aquaculture activities influencing the generation of geosmin and 2-methylisoborneol: a case study in the aquaculture regions of Hongze Lake, China.

Contamination by odor substances such as geosmin (GSM) and 2-methylisoborneol (2-MIB) was examined in the cultured water from aquaculture farming in the region of the Hongze Lake in 2022, and some factors influencing residual levels of them in the water were analyzed. Geographically, high concentrations of GSM were located mainly in the north and northeast culture areas of the lake, while those of 2-MIB were found in the northeast and southwest. Analysis of the water in the enclosure culture revealed significant differences in the concentrations of GSM and 2-MIB among the cultured species. The mean concentrations of GSM in culture water were ranked in the order: crab > the four major Chinese carps > silver and bighead carp, and silver and bighead carp > crab > the four major Chinese carps for 2-MIB. The concentration of GSM was significantly higher at 38.99 ± 18.93 ng/L in crab culture water compared to other fish culture water. Significant differences were observed in GSM concentrations between crab enclosure culture and pond culture, while 2-MIB levels were comparable. These findings suggest that cultural management practices significantly affect the generation of odor substances. The taste and odor (T&O) assessment revealed that the residual levels of GSM and 2-MIB in most samples were below the odor threshold concentrations (OTCs), although high levels of GSM and 2-MIB in all water bodies were at 30.9% and 27.5%, respectively. Compared with the corresponding data from other places and the regulation guidelines of Japan, USA, and China, the region in the Hongze Lake is generally classified as a slightly T&O area, capable of supporting the aquaculture production scale.

Quantitative detection of malachite green in sediment by a time-resolved immunofluorescence method combined with a portable 3D printing equipment platform.

Rapid detection technology of aquaculture fishery drug residues is needed to supplement large-scale instrument methods. To do this, the time-resolved fluorescence immunoassay (TRFIA) method and portable three-dimensional (3D) printing equipment platform were used, in combination with smartphones, to detect malachite green (MG) in pond sediments. The TRFIA was coupled to MG monoclonal antibodies (mAb) through lanthanide metal microspheres europium (Eu3+). The labeled antibody produced competitive immunity in the immune reaction system, and the specific fluorescence intensity in the product was determined by a portable 3D printing equipment platform to achieve quantitative analysis. To test this method, leucomalachite green (LMG) was converted to MG by oxidation of dicyanoquinone (DDQ), and a qualitative analysis was achieved. Methodological evaluation results were satisfactory, recoveries were 83 %-104 %, the limit of detection (LOD) was 0.3 ng/g, the limit of quantitation (LOQ) was 0.7 ng/g, and the coefficient of variation was 1.3 %-7.3 %. The linear equation y = -0.1496x + 0.5585 was in the range of 0-10 ng/g. The linear regression correlation coefficient was 99.2 %. The TRFIA was confirmed and positive samples were measured. Results were consistent with the standard method, which demonstrated that the TRFIA was feasible and that the detection results were reliable. Compared with the national standard method, the TRFIA saves time, is more convenient, and has high sensitivity. It provides an efficient technical method for the rapid screening of MG in the sediments of aquaculture environments.

Balancing the health effect between risks of methylmercury and benefits of nutrients in consumption of Chinese mitten crab (Eriocheir sinensis) in China.

Aquatic products contain both beneficial substances such as polyunsaturated fatty acids and harmful compounds such as methylmercury, and their combined health effects should be assessed at a common end point for a rational analysis. We used a risk-benefit model to assess net IQ point gain from consumption of Chinese mitten crabs (Eriocheir sinensis) from Jiangsu Province. Levels of methylmercury in the edible parts of the crabs ranged from 0.79 to 69.47 µg/kg (mean: 26.88 ± 15.09 µg/kg). Levels of methylmercury did not differ by sampling location. In aquaculture experiments, we simulated artificial pond farming using either iced trash fish or formulated feed. Crabs fed iced trash fish contained 28.72 ± 2.30 mg/g of eicosapentaenoic acid and docosahexaenoic acid and crabs fed formulated feed contained 19.64 ± 3.62 mg/g of eicosapentaenoic acid and docosahexaenoic acid. Model calculations estimated a gain of 7.52 IQ points from consumption of crabs fed formulated feed and 11.00 IQ points from consumption of crabs fed iced trash fish. They are all above the maximum theoretical IQ point gain value. Chinese mitten crab is a kind of high-quality food which is beneficial to the neural development of infants. Our findings may assist in improving crab farming methods and consumption patterns, accelerating the sustainable development of the Chinese mitten crab industry.

Win-win-win pathway for ecological restoration by balancing hydrological, ecological, and agricultural dimensions: Contrasting lessons from highly eroded agroforestry.

Ecological restoration projects (ERP) can effectively reverse ecosystem degradation. However, some ERPs have failed to restore ecosystems under environmental constraints, and they were unable to achieve the desired ecological and economic benefits. To achieve a win-win-win target that balances the hydrological, ecological, and agricultural dimensions, we introduced the contrasting lessons from hotspots of ecosystem restoration in the arid Loess Plateau (LP) and the humid Karst Plateau (KP) in China, and discussed a novel strategy for coordinating ecosystem restoration, water and food security, and residents' livelihoods. The biophysical models and related statistical records showed that aggressive ERPs and soil and water conservation projects (SWCPs) significantly promoted vegetation restoration and reduced soil erosion and sediment yield in both areas. However, excessive afforestation in the arid LP exhausted water resources and threatened ecosystem sustainability. The accelerated replacement of cropland since 1999 in the LP aggravated cropland shortage which led to carbon sequestration and grain productivity declines in the initial years. However, the construction of terrace and check-dam fields and improvements in the conditions of agricultural production reconciled the cropland shortage and stabilized food security. The positive involvement of stakeholders in ERPs effectively minimized land degradation through economic development and the improved livelihoods of local residents. Therefore, based on the evidence from the KP and LP, the proposed win-win-win strategy is potentially applicable in other global regions that suffer from land degradation. This strategy can achieve considerable success if the planners have a good understanding of local environmental conditions as well as the social and economic needs of residents affected by ERPs.

Exploring soil erosion trajectories and their divergent responses to driving factors: a model-based contrasting study in highly eroded mountain areas.

Soil erosion threatens environmental sustainability worldwide. Exploring the trajectories of soil erosion and associated drivers is of great significance for combating land degradation. This study selected the highly eroded Loess Plateau (LP) and Karst Plateau (KP) as contrasting regions to monitor soil erosion dynamics. Monitoring was performed by applying the Revised Universal Soil Loss Equation based on a GIS platform and multi-source input data to investigate associated drivers. The results established that soil erosion in both regions was substantially reduced by ecological restoration projects and significant land use/cover conversions. Landscape and geomorphological variables were found to be the dominant factors controlling soil erosion in the LP and KP, as they influenced land use patches and geomorphological patterns, respectively. The correlations between fragmentation metric indices and soil erosion indicated that the appropriately intensive fragmentation in the LP could mitigate or prevent soil erosion by disturbing its formation and transportation and ultimately positively influenced soil erosion control. Geomorphological patterns were also determinative factors, particularly for the KP, where almost all geomorphological variables were significantly correlated with the erosion modulus. Owing to the peculiar landform and landscape conditions in karst areas and loess hilly-gully areas, geomorphological and landscape variables should be considered when determining the main factors affecting soil erosion processes and integrated into the forecasting model to improve the accuracy of the simulation. The findings of this study are expected to (i) improve the efficacy of soil erosion control and (ii) promote the sustainable planning and management of land and soil resources.

Ecological restoration is not sufficient for reconciling the trade-off between soil retention and water yield: A contrasting study from catchment governance perspective.

Ecological restoration program (ERP) is widely recognized as an effective measure to combat land degradation and improve environmental quality. However, inappropriate ERPs lead to trade-offs between soil retention and water yield as well as conflicts of soil and water resources between the midstream and the downstream of catchment. This study aims to assess the efficiency of ERPs in soil erosion control and identify the trade-offs between soil retention and water yield through the lens of runoff and sediment regimes in contrasting catchments of the Loess Plateau (LP) and the Karst Plateau (KP). Although favorable climate and rapid vegetation restoration substantially reduced water erosion in both these areas, the hydrological responses were not the same because of climate differences. In the arid LP, water and energy variables correlated closely with vegetation cover. Excessive afforestation programs in drylands increased vegetation transpiration and soil evaporation, further exhausting soil water resources, and eventually causing water yield reduction. However, soil and water conservation programs (SWCPs) in the humid KP reduced sediment yield substantially, and the runoff remained stable. Significant runoff reduction in the midstream of the Yellow River aggravated water scarcity and threatened the downstream water demand. Meanwhile, sediment load decline in the LP and the KP impacted sediment deposition in the downstream and estuary formation. From the perspective of integrated catchment governance, human interventions including ERP and SWCP should be more sustainable and consider not only the target process at the local scale (intracoupling effect), but also unprecedented non-target process at the regional scale (telecoupling effect). In addition, it should allow for the supply-demand balance of competing soil and water resources to achieve the coordinated development of resources, environment, and production.

Unfolding the effectiveness of ecological restoration programs in combating land degradation: Achievements, causes, and implications.

Land degradation is one of the most serious environmental problems worldwide. To combat land degradation, China has implemented a series of ecological restoration programs (ERPs). This study selected the northern dryland of China as a case study to examine the efficiency of ERPs, and the response of soil loss to afforestation efforts and climatic conditions was discussed using the principles from the ecological theory of non-linear ecosystem dynamics. Owing to the combined impacts of declining wind speed and rapid vegetation restoration, the soil erosion for the entire region was substantially reduced from 1990 to 2015. However, the rainfall fluctuated considerably, particularly for the period from the late 1990s to early 2000s. Several drought events to some extent inhibited vegetation growth and further offset afforestation efforts, resulting in degradations in vegetation structure and soil retention function, which have been aggravating soil erosion since 2005. In certain representative sandstorm areas, limited increase in rainfall was not enough to promote vegetation growth, and therefore the vegetation cover did not present increasing trends and, in some cases, even declined significantly. The responses in terms of land degradation to climatic conditions and afforestation efforts behaved in a non-linear dynamic manner, providing essential insights into appropriate timings, climate-induced windows of opportunity, and risk in recovering and sustaining ecosystems, and eventually moving towards the land degradation neutrality (LDN) target. The climate-induced windows of opportunity and risk are critical in identifying the time for starting human interventions to mitigate and halt land degradation. Meanwhile, effective investment actions should be taken according to existing environmental conditions and critical thresholds, to achieve LDN at minimum risk and cost.