The cytochrome P450 gene, MdCYP716B1, is involved in regulating plant growth and anthracnose resistance in apple.

Apple is one of the main cultivated fruit trees worldwide. Both biotic and abiotic stresses, especially fungal diseases, have serious effects on the growth and fruit quality of apples. Cytochrome P450, the largest protein family in plants, is critical for plant growth and stress responses. However, the function of apple P450 remains poorly understood. In our previous study, 'Hanfu' autotetraploid showed dwarfism and fungal resistance phenotypes compared to 'Hanfu' diploid. Digital gene expression sequencing analysis revealed that the transcript level of MdCYP716B1 was significantly downregulated in the autotetraploid apple cultivar 'Hanfu'. In this study, we identified and cloned the MdCYP716B1 gene from 'Hanfu' apples. The MdCYP716B1 protein fused to a green fluorescent protein was localized in the cytoplasm. We constructed the plant overexpression vector and RNAi vector of MdCYP716B1, and the apple 'GL-3' was transformed by Agrobacterium-mediated transformation to obtain transgenic plants. Overexpressing and RNAi silencing transgenic plants exhibited an increase and decrease in plant height to 'GL-3', respectively. RNAi silencing transgenic plants displayed increased resistance to Colletotrichum gloeosporioides, whereas overexpression transgenic plants were more sensitive to C. gloeosporioides. According to transcriptome analysis, the transcript levels of gibberellin biosynthesis genes were upregulated in MdCYP716B1-overexpression plants. In contrast with 'GL-3', GA3 accumulation was rose in MdCYP716B1-OE lines and impaired in MdCYP716B1-RNAi lines. Collectively, our data indicate that MdCYP716B1 regulates plant growth and resistance to fungal stress.

Exploring Public Response to COVID-19 on Weibo with LDA Topic Modeling and Sentiment Analysis.

It is necessary and important to understand public responses to crises, including disease outbreaks. Traditionally, surveys have played an essential role in collecting public opinion, while nowadays, with the increasing popularity of social media, mining social media data serves as another popular tool in opinion mining research. To understand the public response to COVID-19 on Weibo, this research collects 719,570 Weibo posts through a web crawler and analyzes the data with text mining techniques, including Latent Dirichlet Allocation (LDA) topic modeling and sentiment analysis. It is found that, in response to the COVID-19 outbreak, people learn about COVID-19, show their support for frontline warriors, encourage each other spiritually, and, in terms of taking preventive measures, express concerns about economic and life restoration, and so on. Analysis of sentiments and semantic networks further reveals that country media, as well as influential individuals and "self-media," together contribute to the information spread of positive sentiment.

MdHAL3, a 4'-phosphopantothenoylcysteine decarboxylase, is involved in the salt tolerance of autotetraploid apple.

KEY MESSAGE: MdHAL3 has PPCDC activity and is involved in the salt tolerance of autotetraploid apple. Apple (Malus × domestica) is the most widely planted fruit tree species worldwide. However, the growth and development of apple have been increasingly affected by abiotic stress, such as high salinity. In our previous study, RNA sequencing (RNA-seq) analysis revealed that the expression level of the MdHAL3 gene was significantly upregulated in the autotetraploid apple cultivar Hanfu. In the present study, we first isolated HAL3, whose product was shown to exert 4'-phosphopantothenoylcysteine decarboxylase (PPCDC) activity, from apple. MdHAL3 was expressed in all organs of apple, and its expression was rapidly induced by salt stress. The MdHAL3 protein was localized to the cytomembrane and cytoplasm. Five MdHAL3 overexpression (OE) lines and five MdHAL3-RNAi apple lines were obtained. We found that MdHAL3 enhanced the salt stress tolerance of apple and that the OE plants rooted more easily than the wild-type (WT) plants. The coenzyme A (CoA) content in the leaves of the OE plants was greater than that in the leaves of the WT plants, and the CoA content in the MdHAL3-RNAi plants was lower than that in the WT plants. Taken together, our findings indicate that MdHAL3 plays an essential role in the response to salt stress in apple.

MdWRKY100 encodes a group I WRKY transcription factor in Malus domestica that positively regulates resistance to Colletotrichum gloeosporioides infection.

Apple (Malus domestica) is an important fruit worldwide; however, the development of the apple industry is limited by fungal disease. Apple bitter rot caused by the pathogen Colletotrichum gloeosporioides is one of the most devastating apple diseases, leading to large-scale losses in apple quality and production. WRKY transcription factors have important functions in the regulation of biotic and abiotic stresses. However, their biological and molecular functions in non-model plants, including apple, remain poorly understood. Here, we isolated MdWRKY100 from 'Hanfu' apple. The MdWRKY100 protein fused to green fluorescent protein localized to the nucleus, and MdWRKY100 in yeast cells displayed transcriptional activation activity, which is consistent with the function of a transcription factor. Additionally, several putative cis-acting elements involved in abiotic stress responsiveness were also identified in the MdWRKY100 promoter. Transcriptional analysis revealed that MdWRKY100 was expressed ubiquitously in all examined apple organs. Overexpression in apple increased resistance to Colletotrichum gloeosporioides, while RNAi silencing transgenic plants were more sensitive to Colletotrichum gloeosporioides. Collectively, our data demonstrate that MdWRKY100 is a positive regulator of Colletotrichum gloeosporioides resistance in apple.