ABSTRACT
OBJECTIVE: To explore the clinical significance of hepatitis B virus large envelope protein (HBV-LP) in diagnosing viral replication, we detected the HBV-LP and HBV DNA in the serum of the patients infected with HBV. METHODS: Serum HBV DNA was detected by fluorescent quantitative PCR (FQ-PCR). HBV-LP was analyzed by enzyme linked immunosorbent assay (ELISA). RESULTS: (1) No significant difference between the detectable rate of HBV DNA and HBV-LP was found in the HBeAg negative patients without of anti-virus treatment. Of all HBeAg negative patients without of anti-virus treatment, the copies of HBV-DNA was related positively to the absorbency HBV-LP. (2) There was significant diference between the detectable rate of HBV DNA and HBV-LP in the HBeAg negative patients after anti-virus treatment. CONCLUSION: There is a perfect correlation between the positive rate of HBV-LP and HBV DNA in the HBeAg negative patients.The HBV DNA is a reliable serological marker that can reflect the replication of HBV in the HBeAg negative patients.
