Extractor-attention-predictor network for quantitative photoacoustic tomography.

Quantitative photoacoustic tomography (qPAT) holds great potential in estimating chromophore concentrations, whereas the involved optical inverse problem, aiming to recover absorption coefficient distributions from photoacoustic images, remains challenging. To address this problem, we propose an extractor-attention-predictor network architecture (EAPNet), which employs a contracting-expanding structure to capture contextual information alongside a multilayer perceptron to enhance nonlinear modeling capability. A spatial attention module is introduced to facilitate the utilization of important information. We also use a balanced loss function to prevent network parameter updates from being biased towards specific regions. Our method obtains satisfactory quantitative metrics in simulated and real-world validations. Moreover, it demonstrates superior robustness to target properties and yields reliable results for targets with small size, deep location, or relatively low absorption intensity, indicating its broader applicability. The EAPNet, compared to the conventional UNet, exhibits improved efficiency, which significantly enhances performance while maintaining similar network size and computational complexity.

​Comprehensive mendelian randomization analysis of plasma proteomics to identify new therapeutic targets for the treatment of coronary heart disease and myocardial infarction.

BACKGROUND: Ischemic heart disease is one of the leading causes of mortality worldwide, and thus calls for development of more effective therapeutic strategies. This study aimed to identify potential therapeutic targets for coronary heart disease (CHD) and myocardial infarction (MI) by investigating the causal relationship between plasma proteins and these conditions. METHODS: A two-sample Mendelian randomization (MR) study was performed to evaluate more than 1600 plasma proteins for their causal associations with CHD and MI. The MR findings were further confirmed through Bayesian colocalization, Summary-data-based Mendelian Randomization (SMR), and Transcriptome-Wide Association Studies (TWAS) analyses. Further analyses, including enrichment analysis, single-cell analysis, MR analysis of cardiovascular risk factors, phenome-wide Mendelian Randomization (Phe-MR), and protein-protein interaction (PPI) network construction were conducted to verify the roles of selected causal proteins. RESULTS: Thirteen proteins were causally associated with CHD, seven of which were also causal for MI. Among them, FES and PCSK9 were causal proteins for both diseases as determined by several analytical methods. PCSK9 was a risk factor of CHD (OR = 1.25, 95% CI: 1.13-1.38, P = 7.47E-06) and MI (OR = 1.36, 95% CI: 1.21-1.54, P = 2.30E-07), whereas FES was protective against CHD (OR = 0.68, 95% CI: 0.59-0.79, P = 6.40E-07) and MI (OR = 0.65, 95% CI: 0.54-0.77, P = 5.38E-07). Further validation through enrichment and single-cell analysis confirmed the causal effects of these proteins. Moreover, MR analysis of cardiovascular risk factors, Phe-MR, and PPI network provided insights into the potential drug development based on the proteins. CONCLUSIONS: This study investigated the causal pathways associated with CHD and MI, highlighting the protective and risk roles of FES and PCSK9, respectively. FES. Specifically, the results showed that these proteins are promising therapeutic targets for future drug development.

Correction: Chen et al. Multifunctional Injectable Hydrogel Loaded with Cerium-Containing Bioactive Glass Nanoparticles for Diabetic Wound Healing. Biomolecules 2021, 11, 702.

The authors would like to replace Figure 4A of the following published paper [...].

Function identification of miR159a, a positive regulator during poplar resistance to drought stress.

Drought seriously affects the growth and development of plants. MiR159 is a highly conserved and abundant microRNA family that plays a crucial role in plant growth and stress responses. However, studies of its function in woody plants are still lacking. Here, the expression of miR159a was significantly upregulated after drought treatment in poplar, and the overexpression of miR159a (OX159a) significantly reduced the open area of the stomata and improved water-use efficiency in poplar. After drought treatment, OX159a lines had better scavenging ability of reactive oxygen species and damage of the membrane system was less than that in wild-type lines. MYB was the target gene of miR159a, as verified by psRNATarget prediction, RT-qPCR, degradome sequencing, and 5' rapid amplification of cDNA ends (5' RACE). Additionally, miR159a-short tandem target mimic suppression (STTM) poplar lines showed increased sensitivity to drought stress. Transcriptomic analysis comparing OX159a lines with wild-type lines revealed upregulation of a series of genes related to response to water deprivation and metabolite synthesis. Moreover, drought-responsive miR172d and miR398 were significantly upregulated and downregulated respectively in OX159a lines. This investigation demonstrated that miR159a played a key role in the tolerance of poplar to drought by reducing stomata open area, increasing the number and total area of xylem vessels, and enhancing water-use efficiency, and provided new insights into the role of plant miR159a and crucial candidate genes for the molecular breeding of trees with tolerance to drought stress.

A new shear creep damage model for rock masses after considering initial damage.

For a long time, one of the important safety problems in open-pit mines is the stability of a large number of high slopes with gently inclined soft interlayer. Rock masses formed after long geological processes generally have some initial damage. Mining works also cause varying degrees of disturbance and damage to rock masses in the mining area during the mining process. This phenomenon means that accurate characterization of the time-dependent creep damage for rock masses under shear load is necessary. The damage variable D is defined based on the spatial and temporal evolution laws of shear modulus and initial level of damage for the rock mass. In addition, a coupling damage equation between the initial damage of the rock mass and shear creep damage is established based on Lemaitre's strain equivalence assumption. Kachanov's damage theory is also incorporated to describe the entire process of time-dependent creep damage evolution for rock masses. A creep damage constitutive model that can reasonably reflect the actual mechanical properties of rock masses under multi-stage shear creep loading conditions is established. This takes into account multi-stage shear creep loading conditions, instantaneous creep damage during the shear load phase, staged creep damage and factors influencing the initial damage of rock masses. The reasonableness, reliability and applicability of this model are verified by comparing the results of the multi-stage shear creep test with calculated values from the proposed model. As opposed to the traditional creep damage model, the shear creep model established in this present study takes into account the initial damage of rock masses and can describe the multi-stage shear creep damage characteristics of rock masses more convincingly.

Advances in the Application of Nanomaterials to the Treatment of Melanoma.

Melanoma can be divided into cutaneous melanoma, uveal melanoma, mucosal melanoma, etc. It is a very aggressive tumor that is prone to metastasis. Patients with metastatic melanoma have a poor prognosis and shorter survival. Although current melanoma treatments have been dramatically improved, there are still many problems such as systemic toxicity and the off-target effects of drugs. The use of nanoparticles may overcome some inadequacies of current melanoma treatments. In this review, we summarize the limitations of current therapies for cutaneous melanoma, uveal melanoma, and mucosal melanoma, as well as the adjunct role of nanoparticles in different treatment modalities. We suggest that nanomaterials may have an effective intervention in melanoma treatment in the future.

The immune-related role of beta-2-microglobulin in melanoma.

Despite the remarkable success of immunotherapy in the treatment of melanoma, resistance to these agents still affects patient prognosis and response to therapies. Beta-2-microglobulin (ß2M), an important subunit of major histocompatibility complex (MHC) class I, has important biological functions and roles in tumor immunity. In recent years, increasing studies have shown that B2M gene deficiency can inhibit MHC class I antigen presentation and lead to cancer immune evasion by affecting ß2M expression. Based on this, B2M gene defect and T cell-based immunotherapy can interact to affect the efficacy of melanoma treatment. Taking into account the many recent advances in B2M-related melanoma immunity, here we discuss the immune function of the B2M gene in tumors, its common genetic alteration in melanoma, and its impact on and related improvements in melanoma immunotherapy. Our comprehensive review of ß2M biology and its role in tumor immunotherapy contributes to understanding the potential of B2M gene as a promising melanoma therapeutic target.

A direct shear apparatus for intact rock under dynamic moisture content.

Under field conditions, the moisture content of rock changes with the weather during prolonged creep. In order to investigate the effect of moisture content change on long-term shear strength and deformation behavior, a shear apparatus for intact rock was developed. Since three prefabricated holes are drilled in the upper part of the rock sample, the water injection device and the gas injection device can be used to inject water and gas into the rock sample alternately during the test to adjust the moisture content without removing the normal load and shear load. By using silicone gasket and seals in the shear box, fluid injection at a pressure of 5 MPa was achieved without leakage. Shear creep tests of argillaceous shale were conducted under both constant and dynamic moisture conditions, and the results were described by the Nishihara model. The experimental results revealed that there are significant differences in the long-term shear strength and deformation of argillaceous shale under different moisture content conditions. The proposed rock shear apparatus can advance the quantitative study of the shear creep properties of rock samples during moisture content changes and has certain practical application value for the prediction of engineering rock mass stability during rainfall.

Effect of Liquid Nitrogen Freeze-Thaw Cycles on Pore Structure Development and Mechanical Properties of Coal.

To improve the mining efficiency of coalbed methane, liquid nitrogen freeze-thawing experiments were performed to improve coal seam permeability and to study its influence on coal pore structure development and mechanical properties. Mechanical properties and nuclear magnetic resonance tests of coal samples were performed with 0, 5, 10, and 15 freeze-thaw cycles of liquid nitrogen. The results show that the number of freeze-thaw cycles caused the change of uniaxial compressive strength and elastic modulus of coal, and the change effect decreased significantly after 11-15 freeze-thaw cycles. Between 0 and 5 freeze-thaw cycles, the base growth rate of the transverse relaxation time T 2 spectral area of the full pore of coal is 44.1%, and that of the transverse relaxation time T 2 spectral area of adsorption pore is 71.5%. After 6-10 freeze-thaw cycles, the fixed base growth rate of the transverse relaxation time T 2 spectral area of the full hole of coal is 269.0%, and the chain growth rate is 156.2%. In this stage, the chain growth rate of the transverse relaxation time T 2 spectral area of the seepage hole is 198.4%, which is mainly the growth of seepage hole volume. After 11-15 freeze-thaw cycles, the chain growth rate of the full pore of coal transverse relaxation time T 2 spectrum area is 20.1%, the chain growth rate of adsorption pore is 4.8%, the chain growth rate of seepage pore is 22.2%, and the growth rate of the pore volume is greatly reduced. Comparing the changes of pore and coal mechanical properties in different pore sizes, it can be seen that the change of adsorption pore volume has a greater impact on coal mechanical properties.

Multifunctional Injectable Hydrogel Loaded with Cerium-Containing Bioactive Glass Nanoparticles for Diabetic Wound Healing.

Diabetic foot wound healing is a major clinical problem due to impaired angiogenesis and bacterial infection. Therefore, an effective regenerative dressing is desiderated with the function of promoting revascularization and anti-bacteria. Herein, a multifunctional injectable composite hydrogel was prepared by incorporation of the cerium-containing bioactive glass (Ce-BG) into Gelatin methacryloyl (GelMA) hydrogel. The Ce-BG was synthesized by combining sol-gel method with template method, which maintained spherical shape, chemical structure and phase constitution of bioactive glass (BG). The Ce-BG/GelMA hydrogels had good cytocompatibility, promoted endothelial cells migration and tube formation by releasing Si ion. In vitro antibacterial tests showed that 5 mol % CeO2-containing bioactive glass/GelMA (5/G) composite hydrogel exhibited excellent antibacterial properties. In vivo study demonstrated that the 5/G hydrogel could significantly improve wound healing in diabetic rats by accelerating the formation of granulation tissue, collagen deposition and angiogenesis. All in all, these results indicate that the 5/G hydrogel could enhance diabetic wound healing. Therefore, the development of multifunctional materials with antibacterial and angiogenic functions is of great significance to promote the repair of diabetic wound healing.