ABSTRACT
OBJECTIVE: This study aimed to determine mitochondrial mRNA expression levels and the relationships between these expression levels and various adverse clinicopathological characteristics. METHODS: The mRNA expression levels of all 12 genes encoded protein, located on the heavy-strand of mitochondrial DNA including cytochrome b, NADH1, NADH2, NADH3, NADH4, NADH4L, NADH5, ATPase6, ATPase8, cytochrome c oxidase subunit 1, cytochrome c oxidase subunit 2, cytochrome c oxidase subunit 3 were analyzed in 30 head and neck squamous cell carcinoma (HNSCC) and the corresponding normal tissues using reverse transcriptase quantitative real time PCR. Pearson Chi-square test was used to determine the relationships between these expression levels and categorical parameters. RESULTS: The expression levels of 12 mitochondrial mRNAs were observed in all 30 HNSCC patients with down-regulation, ranging from 43.3% to 76.7% and up-regulation, ranging from 10.0% to 36.7%. Furthermore, the number of cases with down-regulations in all 6 NADH and cytochrome b mRNA with TMN stages III and IV were significantly higher than that in stages I and II (p=0.049 and 0.007, respectively). CONCLUSION: Down-regulation of all mitochondrial NADH mRNA as well as mitochondrial cytochrome b mRNA was associated with high tumor stage among HNSCC patients.
Subject(s)
Cytochromes b/genetics , Down-Regulation , Gene Expression Regulation, Neoplastic , Mitochondria/genetics , NAD/genetics , Squamous Cell Carcinoma of Head and Neck/genetics , Adult , Aged , Aged, 80 and over , Cytochromes b/metabolism , DNA, Mitochondrial , Electron Transport Complex IV/genetics , Female , Genome, Mitochondrial/genetics , Humans , Male , Middle Aged , Mitochondria/metabolism , Mitochondrial Proton-Translocating ATPases/genetics , NAD/metabolism , Neoplasm Staging , RNA, Messenger/genetics , RNA, Messenger/metabolism , RNA, Mitochondrial/genetics , RNA, Mitochondrial/metabolismABSTRACT
PURPOSE: To determine the adhesion of resin-modified glass-ionomer cement to bovine dentin under contaminated and decontaminated conditions. MATERIALS AND METHODS: Forty-five bovine mandibular incisors were used. The surfaces of bovine dentin specimens were subjected to Temp-bond, dental handpiece lubricant (contamination), Hibiscrub, chlorhexidine or pumice (decontamination), as well as contamination followed by decontamination. From these, 14 test groups were created to investigate the effects of these variables on the microtensile bond strength of a resin-modified glassionomer cement to dentin. In addition, scanning electron microscopy was performed to examine the effects of contamination and decontamination procedures on the dentin surfaces. The data were analyzed by one-way ANOVA, Kruskal-Wallis, and Mann-Whitney tests. RESULTS: SEM examination showed visible differences between the control group and dentin contaminated with Temp-bond or handpiece lubricant. All the contamination and decontamination test agents when used alone - except Hibiscrub - showed significant reductions in bond strength when compared to the control (p < 0.001). All the test groups subjected to contamination followed by decontamination showed a significantly reduced bond strength (p < 0.001) when compared to the control, with the exception of the handpiece lubricant/Hibiscrub combination. CONCLUSION: Under the conditions tested, Temp-bond, handpiece lubricant, chlorhexidine, and pumice may have an adverse effect on the bonding of resin-modified glass ionomer to dentin. Hibiscrub was effective in decontaminating handpiece lubricant but not Temp-bond.
Subject(s)
Anti-Infective Agents, Local/chemistry , Dental Bonding , Dentin/ultrastructure , Glass Ionomer Cements/chemistry , Resin Cements/chemistry , Acid Etching, Dental/methods , Acrylic Resins/chemistry , Adhesiveness , Animals , Cattle , Chlorhexidine/analogs & derivatives , Chlorhexidine/chemistry , Decontamination , Dental Stress Analysis/instrumentation , Eugenol/chemistry , Hydrocarbons/chemistry , Lubricants/chemistry , Materials Testing , Microscopy, Electron, Scanning , Mouthwashes/chemistry , Random Allocation , Silicates/chemistry , Stress, Mechanical , Surface Properties , Tensile Strength , Zinc Oxide/chemistry , Zinc Oxide-Eugenol Cement/chemistryABSTRACT
Oral cancer ranks as one of the top ten cancers in Thailand. Molecular carcinogenesis of this disease remains unknown. The purpose of this report was to identify the genetic alteration profile in Thai oral squamous cell carcinoma (OSCC) patients using arbitrarily primed PCR and to determine the association between genetic alterations and clinico-pathological characteristics. Band alteration profiles in the 32 OSCC tissues were compared with corresponding normal tissues amplified from 60 arbitrary primers using arbitrarily primed polymerase chain reaction (AP-PCR) were identified with 12 primers. Among these, 45 band patterns presented the alteration ranged from 36% to 88%. Primer AD15 at 750 base pairs (AD15-750 bp) was found to have both the highest band alteration (88%) and the highest band loss (37%). The highest DNA band amplification was found in primer AX11-1300 bp (56%). Primer AX-11 at 1300 base pairs at the altered frequency of 78% was significantly associated with smoking (p=0.007), and primer N20 at 800 base pairs showed association with low grade tumors (p=0.030). Our results indicate that AP-PCR is a useful technique for detect genetic alteration in oral squamous cell carcinoma and provide various genetic alternative data.
