ABSTRACT
The strength and rigidity of base course can significantly affect the performance of pavements. The rigidity of roadways relies on the infill material used in base layers which is interdependent on its thickness and quality. With the increase in the base thickness, the performance of the base course improves but the cost associated with it also increases. Since the natural aggregates are not adequately available, use of waste materials in road construction can prove economical and environmental friendly. In this study, efficacy of geosynthetic (geocells and non-woven geotextile) reinforced quarry waste as an alternative base course materials (BCM) were investigated under static loading conditions (plate load test-PLT). By increasing the geocell height from 100 to 150 mm, the bearing capacity (BC) increased from 450 to 840 kPa. Similarly due to combined use of geocell and geotextile, BC increased from 500 to 890 kPa. The experimental results depict that the geosynthetic reinforcement increases the load bearing capacity of QWB's by 85%. Moreover, the artificial neural network analysis (ANN) was performed to predict the deformation on top of footing while considering different influential parameters. The results obtained from the ANN analysis were in good fit.
Subject(s)
Waste ProductsABSTRACT
Ependymomas are common childhood brain tumours that occur throughout the nervous system, but are most common in the paediatric hindbrain. Current standard therapy comprises surgery and radiation, but not cytotoxic chemotherapy as it does not further increase survival. Whole-genome and whole-exome sequencing of 47 hindbrain ependymomas reveals an extremely low mutation rate, and zero significant recurrent somatic single nucleotide variants. Although devoid of recurrent single nucleotide variants and focal copy number aberrations, poor-prognosis hindbrain ependymomas exhibit a CpG island methylator phenotype. Transcriptional silencing driven by CpG methylation converges exclusively on targets of the Polycomb repressive complex 2 which represses expression of differentiation genes through trimethylation of H3K27. CpG island methylator phenotype-positive hindbrain ependymomas are responsive to clinical drugs that target either DNA or H3K27 methylation both in vitro and in vivo. We conclude that epigenetic modifiers are the first rational therapeutic candidates for this deadly malignancy, which is epigenetically deregulated but genetically bland.
Subject(s)
CpG Islands/genetics , Ependymoma/genetics , Epigenesis, Genetic/genetics , Animals , Brain Neoplasms/drug therapy , Brain Neoplasms/genetics , DNA Methylation/drug effects , Embryonic Stem Cells/metabolism , Ependymoma/drug therapy , Epigenomics , Female , Gene Expression Regulation, Neoplastic , Gene Silencing/drug effects , Histones/drug effects , Histones/metabolism , Humans , Infant , Mice , Mice, Inbred NOD , Mice, SCID , Mutation/genetics , Phenotype , Polycomb Repressive Complex 2/metabolism , Prognosis , Rhombencephalon/pathology , Xenograft Model Antitumor AssaysABSTRACT
A significant frequency of mutations (six missense and one silent) was found, for the first time, at the coding region of the bak gene (exons 3, 4 and 6) in 42 carcinomas of the uterine cervix, while no mutations were detected in 32 non-neoplastic cervix tissues. Bak mutations were observed more frequently in the advanced stage and mutated cancer tissues were more resistant to radiotherapy, although trends were not statistically significant because of small sample size.
